Published: Jan. 1, 2024
Language: Английский
Microchemical Journal, Journal Year: 2024, Volume and Issue: 204, P. 111157 - 111157
Published: Sept. 1, 2024
Language: Английский
Citations
3
Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(4), P. 369 - 369
Published: March 29, 2024
Patients with COVID-19 infection have distinct oropharyngeal microbiota composition and diversity metrics according to disease severity. However, these findings are not consistent across the literature. We conducted a multicenter, prospective study in patients requiring outpatient versus inpatient management explore microbial abundance of taxa at phylum, family, genus, species level, we utilized alpha beta indices further describe our findings. collected washing specimens time entry, which coincided diagnosis, conduct all analyses. included 43 study, whom 16 were managed as outpatients 27 required hospitalization. Proteobacteria, Actinobacteria, Bacteroidetes, Saccharibacteria TM7, Fusobacteria, Spirochaetes most abundant phyla among patients, while 61 different families detected, Streptococcaceae Staphylococcaceae predominant. A total 132 genera Streptococcus being predominant genus outpatients, contrast hospitalized Staphylococcus was LeFSe analysis identified 57 washings participants that could discriminate severity symptoms infections. Alpha did reveal difference bacterial between groups, but established communities inpatients outpatients. Our provides information on complex association SARS-CoV-2 infection. Although cannot establish causation, knowledge specific taxonomic changes increasing can provide us novel clues for prognostic classification patients.
Language: Английский
Citations
1
Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2570 - 2570
Published: Dec. 13, 2024
The human respiratory tract is colonized by a complex microbial community that helps maintain health and plays crucial role in defending the host from infections. Respiratory viruses have been demonstrated to alter microbiota composition, resulting opportunistic species expansion, increasing disease severity susceptibility bacterial co-infections. This study aims examine compositional differences nasal between SARS-CoV-2-infected non-infected patients. We conducted Oxford Nanopore full-length 16S rRNA sequencing on swabs 94 COVID-19 negative 85 positive patients collected during SARS-CoV-2 pandemic Malta. Our analysis identified significant alpha beta diversity composition among our groups. observed trend toward decreased richness evenness COVID-Positive cohort with increased abundance of common including Citrobacter koseri, Dolosigranulum pigrum, Haemophilus influenzae, Klebsiella pneumoniae, Moraxella catarrhalis. findings this are line previously published papers identifying key alterations associated infection. Understanding these microbiome-driven mechanisms could present novel prognostic markers or offer new approaches for prevention treatment.
Language: Английский
Citations
1
Published: Jan. 1, 2024
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Language: Английский
Citations
0
Published: Jan. 1, 2024
Transcription-polymerase chain reaction (RT-qPCR) is the golden standard to detect viral RNA. However, it suffers from several inherent defects such as high false-negative rates, time-consuming and expensive. To exploit better analytical methods for future global pandemics, vital develop rapid, sustainable, environmentally-friendly sensing methods. Here, a one-step, regenerated nanosensor assembled by DNA tweezers developed rapid sustainable detection of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA selected model analyte. The tweezer designed with central strand two arm strands containing overhangs complementary SARS-CoV-2 hybridizes strains closed conformation, resulting in close proximity donor acceptor fluorophores. With addition an anti-strand RNA, relaxed open conformation through displacement process, leading separation dual Thus, revived fresh proposed can be three cycles same performance. Moreover, detected within 30 minutes limit 0.04 nM. By altering sequences tweezer, sensitive, Hg2+ achieved. provides novel approach flexible, Hg2+, exhibiting great potential routine preliminarycoronavirus disease tests, epidemic control, environmental monitoring.
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0