Cryopreservation, cryoprotectants, and potential risk of epigenetic alteration
Journal of Assisted Reproduction and Genetics,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 22, 2024
Language: Английский
Strengths and limitations of reduced representation bisulfite sequencing (RRBS) in the perspective of DNA methylation analysis in fish: a case-study on rainbow trout spermatozoa
Fish Physiology and Biochemistry,
Journal Year:
2024,
Volume and Issue:
50(5), P. 2067 - 2082
Published: March 1, 2024
Language: Английский
Influence of Dietary Fatty Acids on Fish Sperm Tolerance to Cryopreservation
Reviews in Aquaculture,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 9, 2024
ABSTRACT
To
meet
growing
fish
demand,
aquaculture
must
develop
sustainable,
cost‐effective,
and
high‐performance
feed
formulations
for
industry
advancement.
Plant‐based
lipids
are
a
viable
alternative
to
forage
ingredients
in
finfish
diets.
However,
most
plant‐derived
lack
long‐chain
polyunsaturated
fatty
acids
(LC‐PUFA)
like
EPA
DHA,
which
nutritionally
essential
fish.
This
review
summarizes
recent
findings
on
the
nutritional
aspects
of
brood
stock
sperm
quality,
emphasizing
effects
reproductive
outcomes.
Studies
indicate
that
substituting
oil
with
plant
oils
alters
acid
profiles
tissues
gametes,
potentially
compromising
cryopreservation
success,
progeny
viability.
Sperm
membrane
lipids,
including
EPA,
ARA,
vital
motility
fertilization
rates
post‐thaw.
Additionally,
has
been
linked
molecular
epigenetic
alterations
sperm,
may
negatively
affect
offspring
quality.
The
role
mRNA
noncoding
RNA
regulating
function
embryonic
development
is
well
known,
thus
any
damage
these
molecules
as
consequence
might
induce
long‐term
offspring.
Proteomic
analyses
reveal
can
lead
significant
protein
loss
diminishing
their
functional
capabilities.
Optimizing
cryoprotectant
protocols
freezing
techniques
crucial
reduce
damage,
while
dietary
play
key
preserving
quality
during
stress.
Further
research
omic
technologies
fully
understand
tolerance
cryopreservation,
will
improve
outcomes
enhance
sustainability
aquaculture.
Language: Английский
Paternal-effect-genes revealed through sperm cryopreservation in Perca fluviatilis
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: March 16, 2024
Knowledge
about
paternal-effect-genes
(PEGs)
(genes
whose
expression
in
the
progeny
is
influenced
by
paternal
factors
present
sperm)
fish
very
limited.
To
explore
this
issue,
we
used
milt
cryopreservation
as
a
specific
challenge
test
for
sperm
cells,
thus
enabling
selection
amidst
cryo-sensitivity.
We
created
two
groups
of
Eurasian
perch
(Perca
fluviatilis)
model
-
eggs
fertilized
either
with
fresh
(Fresh
group)
or
cryopreserved
(Cryo
from
same
male
followed
phenotypic-transcriptomic
examination
consequences
obtained
(at
larval
stages).
Most
phenotypical
observations
were
similar
both
groups,
except
final
weight
which
was
higher
Cryo
group.
Milt
appeared
to
act
"positive
selection"
factor,
upregulating
most
PEGs
Transcriptomic
profile
freshly
hatched
larvae
sourced
genes
involved
development
visual
perception
and
identified
them
PEGs.
Consequently,
group
exhibited
enhanced
eyesight,
potentially
contributing
more
efficient
foraging
gain
compared
Fresh
This
study
unveils,
first
time,
significant
influence
genome
on
system
fish,
highlighting
pde6g,
opn1lw1,
rbp4l
novel
Language: Английский
Cryopreservation of common carp (Cyprinus carpio L.) sperm: Insights into low-ionic cryoprotective medium mechanism and efficacy
Aleksandra Krasilnikova,
No information about this author
Marek Rodina,
No information about this author
David Gela
No information about this author
et al.
Aquaculture,
Journal Year:
2024,
Volume and Issue:
unknown, P. 741807 - 741807
Published: Oct. 1, 2024
Language: Английский
High temperature induces motility of zebrafish sperm after short-term storage
Aquaculture,
Journal Year:
2024,
Volume and Issue:
595, P. 741623 - 741623
Published: Sept. 13, 2024
Language: Английский
Cryopreservation of yellowtail kingfish (Seriola lalandi) sperm: Effects on metabolic enzymes and sperm quality
Aquaculture,
Journal Year:
2024,
Volume and Issue:
596, P. 741865 - 741865
Published: Nov. 12, 2024
Language: Английский
Cryopreservation did not affect spermatogonia global methylation profile in Senegalese sole (Solea senegalensis)
Cryobiology,
Journal Year:
2024,
Volume and Issue:
118, P. 105162 - 105162
Published: Nov. 23, 2024
Language: Английский
Paternal-effect genes revealed through semen cryopreservation inPerca fluviatilis
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 7, 2023
Abstract
Knowledge
about
paternal-effect
genes
(the
expression
of
which
in
progeny
is
controlled
by
the
paternal
genome)
fish
very
limited.
To
explore
this
issue,
we
used
semen
cryopreservation
as
a
specific
challenge
test
for
sperm
cells,
thus
enabling
selection
amidst
cryo-sensitivity.
We
created
two
groups
Eurasian
perch
(
Perca
fluviatilis
)
model
–
eggs
fertilized
either
with
fresh
(Fresh
group)
or
cryopreserved
(Cryo
from
same
male
followed
zootechnical-transcriptomic
examination
consequences
obtained
(at
larval
stages).
Most
zootechnical
observations
were
similar
both
groups,
except
final
weight
was
higher
Cryo
group.
Semen
appeared
to
act
“positive
selection”
factor,
upregulating
most
Transcriptomics
profile
freshly
hatched
larvae
sourced
involved
development
visual
perception
genes.
Consequently,
group
exhibited
enhanced
eyesight,
potentially
contributing
more
efficient
foraging
and
gain
compared
Fresh
This
study
unveils,
first
time,
significant
influence
genome
on
system
fish,
highlighting
pde6g
,
opn1lw1
rbp4l
novel
Language: Английский