Transcriptional Responses and Microbiome Shifts in Pea Roots Under High pH Stress DOI Creative Commons

Asha Thapa,

Md Rokibul Hasan, Bulbul Ahmed

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract High soil pH severely impacts plant growth and productivity, yet the transcriptomic changes microbial dynamics underlying stress adaptation in garden pea ( Pisum sativum ssp. hortense ) remain unclear. This study demonstrates that high leads to stunted growth, reduced biomass, impaired photosynthesis, nutrient status pea. Further, disruption key nitrogen-fixing bacteria Rhizobium indicum, R. leguminosarum, redzepovicii ), along with downregulation of NifA NifD genes upregulation NifH nodules highlight critical role micronutrient balance legume-microbe symbiosis a compensatory response maintain nitrogen status. RNA-seq analysis revealed extensive transcriptional reprogramming roots, characterized by oxidative (e.g., oxidoreductase glutathione transferase activities, metal ion transporters) related ammonia-lyase activity binding, reflecting broader disruptions homeostasis. KEGG pathway identified enrichment MAPK signaling pathway, likely interacting other pathways associated tolerance, metabolic adjustment, structural reorganization as part adaptive responses pH. Root microbiome showed significant Variovorax, Shinella, Chaetomium , suggesting host-driven recruitment under stress. Stable genera such Pseudomonas, Novosphingobium, Mycobacterium, Herbaspirillum, Paecilomyces displayed resilience conditions, potentially forming core components for In targeted study, inoculation plants an enriched microbiome, particularly C. globosum conditions improved parameters increased abundance Stenotrophomonas Pseudomonas roots. It suggests these bacterial may act helper microbes collectively promoting suffering from These findings provide foundation microbiome-aided breeding programs development consortia enhance conditions.

Language: Английский

Roots of synthetic ecology: microbes that foster plant resilience in the changing climate DOI
Ekaterina Kozaeva, Abdul Aziz Eida, Ella F Gunady

et al.

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 88, P. 103172 - 103172

Published: July 18, 2024

Language: Английский

Citations

4
Impact of anoxygenic phototrophic bacteria on the growth of Arabidopsis thaliana under salt stress DOI Creative Commons

BEHİYE NUR AKSOY,

Hamza Ettadili, Caner Vural

et al.

TURKISH JOURNAL OF BOTANY, Journal Year: 2025, Volume and Issue: 49(1), P. 25 - 39

Published: Jan. 9, 2025

The effect of a microbial mixture containing anoxygenic phototrophic bacteria (APB) on the growth Arabidopsis thaliana ecotype Col-0 under salt stress was investigated. To induce stress, 150 mM NaCl applied to A. thaliana. Four treatments were employed assess impact APB: (1) control plants, (2) plants inoculated with enriched photosynthetic bacteria, (3) and irrigated solution, (4) solution. analysis demonstrated that exhibited increased leaf formation leafsize. Furthermore, an increase in chlorophyll content observed all except treatment4. Quantitative genes related early responses thaliana, including ROS scavenging (APX2), ABA signalling (RD29A RD29B), jasmonate synthesis (LOX2), conducted by RT-qPCR. During experiment, metabolic activity soil determined measuring 1,3,5-triphenyl formazan (TFF). Metagenomic performed soils 2 3 after 10 days, bacterial diversity determined. dominant genera Pseudomonas, Variovorax, Azospirillum, Methylophilus, Flavobacterium, Acidovorax, Hydrogenophaga, Mucilaginibacter, Rhodanobacter. Among genera, proportion APB purple non-sulfur (PNSB), 15.3% 18.8% 3, respectively.

Language: Английский

Citations

0
Association Analysis of the Genomic and Functional Characteristics of Halotolerant Glutamicibacter endophyticus J2-5-19 from the Rhizosphere of Suaeda salsa DOI Creative Commons
Longhao Sun, S. S. Sun,

Tianyang Liu

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 208 - 208

Published: Jan. 18, 2025

Halotolerant plant growth-promoting bacteria (HT-PGPB) have attracted considerable attention for their significant potential in mitigating salt stress crops. However, the current exploration and development of HT-PGPB remain insufficient to meet increasing demands agriculture. In this study, an isolated from coastal saline-alkali soil Yellow River Delta was identified as Glutamicibacter endophyticus J2-5-19. The strain capable growing media with up 13% NaCl producing proteases, siderophores, hormone IAA. Under 4‰ stress, inoculation J2-5-19 significantly increased wheat seed germination rate 37.5% 95%, enhanced dry weight maize seedlings by 41.92%, notably improved root systems. Moreover, work presented first whole-genome endophyticus, revealing that G. resisted expelling sodium ions taking potassium through Na+/H+ antiporters uptake proteins, while also accumulating compatible solutes such betaine, proline, trehalose. Additionally, genome contained multiple key genes, including those involved IAA biosynthesis, siderophore production, GABA synthesis. findings provide a theoretical foundation microbial resources specialized inoculants soils.

Language: Английский

Citations

0
Genomic characterization and computational phenotyping of agriculturally important microbes DOI

Ahmed Abdul Haleem Khan

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 301 - 314

Published: Jan. 1, 2025

Language: Английский

Citations

0
Transcriptional reprogramming and microbiome dynamics in garden pea exposed to high pH stress during vegetative stage DOI

Asha Thapa,

Md Rokibul Hasan, Ahmad Humayan Kabir

et al.

Planta, Journal Year: 2025, Volume and Issue: 261(4)

Published: March 10, 2025

Language: Английский

Citations

0
Exiguobacterium aurantiacumSA100 induces antioxidant enzymes and salinity tolerance gene expression in wheat DOI
Soheila Aghaei Dargiri, Shahram Naeimi, Ali Movahedi

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(3)

Published: May 1, 2025

Abstract This study evaluated the effects of Exiguobacterium aurantiacum SA100 on wheat ( Triticum aestivum ) growth under varying levels salinity stress. Results indicated that significantly enhanced seed germination, root and shoot length, fresh dry biomass across levels, particularly at 50 100 mM NaCl. Inoculation improved antioxidant enzyme activities (CAT, APX, POD, PPO), increased total phenolic content, reduced oxidative damage by lowering MDA H 2 O 150 salinity. Ionic balance was maintained, with significant increases in K + , Mg ++ Ca a reduction Na accumulation. Gene expression analysis revealed upregulation salt‐tolerance genes NAC7 NHX1 SOS1 downregulation stress‐responsive GS1 DREB2 DHN13 WRKY32 ). Principal component confirmed promotes tolerance modulating both biochemical molecular responses. These findings suggest E. as promising bioinoculant for enhancing resilience

Language: Английский

Citations

0
Regulatory mechanisms of plant rhizobacteria on plants to the adaptation of adverse agroclimatic variables DOI Creative Commons
Krishan K. Verma, Abhishek Joshi,

Xiu-Peng Song

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 23, 2024

The mutualistic plant rhizobacteria which improve development and productivity are known as growth-promoting (PGPR). It is more significant due to their ability help the plants in different ways. main physiological responses, such malondialdehyde, membrane stability index, relative leaf water content, photosynthetic gas exchange, chlorophyll fluorescence efficiency of photosystem-II, pigments observed during unfavorable environmental conditions. Plant one crucial chemical messengers that mediate response stressed interaction with essential nutrition can enhance agricultural sustainability various genotypes or cultivars. Rhizobacterial inoculated induce biochemical variations resulting increased stress resistance efficiency, defined induced systemic resistance. Omic strategies revealed inoculation caused upregulation stress-responsive genes—numerous recent approaches have been developed protect from threats. microbes compounds they secrete constitute valuable biostimulants play roles regulating mechanisms. present review summarized developments functional characteristics action mechanisms sustaining production under conditions, special attention on rhizobacteria-mediated molecular responses associated stress-induced responses.

Language: Английский

Citations

1
Bacterial community composition of wheat aboveground compartments correlates with yield during the reproductive phase DOI
Xiao Fu,

Yuying Ma,

Teng Yang

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Plant-associated microbial communities play important roles in agricultural productivity, and their composition has been shown to vary across plant compartments developmental stages. However, the response of within different at stages diverse long-term fertilization treatments, as well linkages with crop yields, remains underexplored. This study analyzed wheat-associated bacterial various soil under three treatments throughout vegetative reproductive phases. The variance community was primarily attributed compartments, followed by belowground (bulk soil, rhizosphere root) exhibited stronger responses than aboveground (stem leaf). responded all stages, it significantly correlated yields during phase, whereas only showed a which point yields. Moreover, this co-occurrence network enhanced complexity, contained an increased number keystone species associated such

Language: Английский

Citations

1
Transcriptional Responses and Microbiome Shifts in Pea Roots Under High pH Stress DOI Creative Commons

Asha Thapa,

Md Rokibul Hasan, Bulbul Ahmed

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract High soil pH severely impacts plant growth and productivity, yet the transcriptomic changes microbial dynamics underlying stress adaptation in garden pea ( Pisum sativum ssp. hortense ) remain unclear. This study demonstrates that high leads to stunted growth, reduced biomass, impaired photosynthesis, nutrient status pea. Further, disruption key nitrogen-fixing bacteria Rhizobium indicum, R. leguminosarum, redzepovicii ), along with downregulation of NifA NifD genes upregulation NifH nodules highlight critical role micronutrient balance legume-microbe symbiosis a compensatory response maintain nitrogen status. RNA-seq analysis revealed extensive transcriptional reprogramming roots, characterized by oxidative (e.g., oxidoreductase glutathione transferase activities, metal ion transporters) related ammonia-lyase activity binding, reflecting broader disruptions homeostasis. KEGG pathway identified enrichment MAPK signaling pathway, likely interacting other pathways associated tolerance, metabolic adjustment, structural reorganization as part adaptive responses pH. Root microbiome showed significant Variovorax, Shinella, Chaetomium , suggesting host-driven recruitment under stress. Stable genera such Pseudomonas, Novosphingobium, Mycobacterium, Herbaspirillum, Paecilomyces displayed resilience conditions, potentially forming core components for In targeted study, inoculation plants an enriched microbiome, particularly C. globosum conditions improved parameters increased abundance Stenotrophomonas Pseudomonas roots. It suggests these bacterial may act helper microbes collectively promoting suffering from These findings provide foundation microbiome-aided breeding programs development consortia enhance conditions.

Language: Английский

Citations

0