Impact of KLF4, SHH, and Hif1a Knockdown on miRNA Expression in Malign Melanoma Cancer Stem Cells DOI Open Access
Berrin Özdil, Çığır Biray Avcı, Hüseyin Aktuğ

et al.

Ege Tıp Dergisi, Journal Year: 2024, Volume and Issue: 63(4), P. 595 - 602

Published: Dec. 9, 2024

Aim: microRNAs (miRNAs) play a pivotal role in gene regulation, influencing various cellular processes such as differentiation, proliferation, and apoptosis. This study investigated the expression of three specific miRNAs (Hsa-miR-21-5p, Hsa-miR-9-5p, Hsa-miR-200a-5p) malign melanoma stem cells (CSCs) non-stem (NSCs). Method: CSCs NCSCs were sorted from CHL-1 based on CD133 marker, malignant cell line. CD133+ treated with Hif1a, KLF4, SHH siRNA levels different compared between groups. Results: Our results revealed that Hsa-miR-200a-5p was similar both Conversely, Hsa-miR-21-5p Hsa-miR-9-5p significantly upregulated NCSCs. Further analysis showed knockdown KLF4 did not affect these miRNAs. However, silencing resulted substantial downregulation significant upregulation Hsa-miR-9-5p. Additionally, Hif1a led to hsa-miR-9-5p. Conclusion: These findings highlight complex regulatory mechanisms miRNA contexts suggest potential roles for response silencing.

Language: Английский

A Novel Gene Signature for Predicting Outcome in Colorectal Cancer Patients Based on Tumor Cell-Endothelial Cell Interaction via Single-Cell Sequencing and Machine Learning DOI Creative Commons

Lina Pang,

Qi Sun, Wenyue Wang

et al.

Heliyon, Journal Year: 2025, Volume and Issue: unknown, P. e42237 - e42237

Published: Jan. 1, 2025

Language: Английский

Citations

0
The distinctive signature of regulatory CD4 T cells committed in the human thymus DOI Creative Commons
Alexandre A. S. F. Raposo, Susana Paço, Miguel Ângelo‐Dias

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Thymically committed regulatory CD4 T cells (tTregs) are essential for immune homeostasis and self-tolerance. We established the human tTreg Expression Signature by comparing genome-wide transcriptomic profiles between tTregs their conventional counterparts (tTconvs). further exploited high sequencing depth of our bulk RNA-seq data to identify a subset 250 genes significantly expressed in with neglectable expression tTconvs, defined as below levels IL2RA , that we named thymic Treg “private” genes. Notably, pathways related cell motility, inflammation, T-cell effector specification were overrepresented within private found 163 these less circulating naïve memory Tregs when compared peripheral generated parallel. This result suggested higher activity most thymus compartments. Altogether, provide unique resource inform future studies, such improving annotation single-cell spatial transcriptional data, or help designing studies validate putative biomarkers thymically Tregs, priority field.

Language: Английский

Citations

0
Exploring the molecular mechanisms of macrophages in islet transplantation using single-cell analysis DOI Creative Commons
Zuhui Pu, Shujuan Chen, Ying Lü

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 29, 2024

Background Islet transplantation is a promising treatment for type 1 diabetes that aims to restore insulin production and improve glucose control, but long-term graft survival remains challenge due immune rejection. Methods ScRNA-seq data from syngeneic allogeneic islet grafts were obtained GSE198865. Seurat was used filtering clustering, UMAP dimension reduction. Differentially expressed genes analyzed between grafts. Gene set variation analysis (GSVA) performed on the HALLMARK gene sets MSigDB. Monocle 2 reconstruct differentiation trajectories, cytokine signature enrichment compare responses Results Three distinct macrophage clusters (Mø-C1, Mø-C2, Mø-C3) identified, revealing complex interactions regulatory mechanisms within populations. The significant activation of macrophages in transplants marked by upregulation allograft rejection-related pathways involved inflammatory interferon responses. GSVA revealed eight significantly upregulated Mø-C2 cluster. Trajectory Mø-C3 serves as common progenitor, branching into Mø-C1 Mø-C2. Cytokine differences responses, highlighting immunological environments created Conclusion This study advances understanding roles context cellular fate processes. findings highlight potential therapeutic targets enhancing function, emphasizing importance aspects transplant acceptance longevity.

Language: Английский

Citations

1
Impact of KLF4, SHH, and Hif1a Knockdown on miRNA Expression in Malign Melanoma Cancer Stem Cells DOI Open Access
Berrin Özdil, Çığır Biray Avcı, Hüseyin Aktuğ

et al.

Ege Tıp Dergisi, Journal Year: 2024, Volume and Issue: 63(4), P. 595 - 602

Published: Dec. 9, 2024

Aim: microRNAs (miRNAs) play a pivotal role in gene regulation, influencing various cellular processes such as differentiation, proliferation, and apoptosis. This study investigated the expression of three specific miRNAs (Hsa-miR-21-5p, Hsa-miR-9-5p, Hsa-miR-200a-5p) malign melanoma stem cells (CSCs) non-stem (NSCs). Method: CSCs NCSCs were sorted from CHL-1 based on CD133 marker, malignant cell line. CD133+ treated with Hif1a, KLF4, SHH siRNA levels different compared between groups. Results: Our results revealed that Hsa-miR-200a-5p was similar both Conversely, Hsa-miR-21-5p Hsa-miR-9-5p significantly upregulated NCSCs. Further analysis showed knockdown KLF4 did not affect these miRNAs. However, silencing resulted substantial downregulation significant upregulation Hsa-miR-9-5p. Additionally, Hif1a led to hsa-miR-9-5p. Conclusion: These findings highlight complex regulatory mechanisms miRNA contexts suggest potential roles for response silencing.

Language: Английский

Citations

0