EFTUD2 is a promising diagnostic and prognostic indicator involved in the tumor immune microenvironment and glycolysis of lung adenocarcinoma DOI Creative Commons
Ankang Yin,

Yufan Xu,

Xiyang Su

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: April 1, 2025

Elongation Factor Tu GTP Binding Domain Containing 2 (EFTUD2), a conserved spliceosomal GTPase, is involved in craniofacial development and various cancers, but its role lung adenocarcinoma (LUAD) remains unclear. EFTUD2 expression LUAD tissues was analyzed using data from TCGA GEO, validated by immunohistochemistry, RT-qPCR, Western blotting. The relationship between clinical features examined Fisher's exact test. Diagnostic prognostic analyses were performed R. Hub genes related to identified through topological algorithms, immune infiltration assessed CIBERSORT. cGAS-STING pathway m6A modification also the cohort. Functional assays conducted assess EFTUD2's impact on cell proliferation, cycle, invasion, metastasis, while glycolytic enzyme levels measured upregulated cells, correlating with N classification, visceral pleural intravascular tumor embolism, cytokeratin-19 fragment antigen 21-1. Sixteen EFTUD2-related hub identified. Higher linked altered infiltration, increased TumorPurity scores decreased StromalScore, ImmuneScore, ESTIMATEScore values. Gene enrichment highlighted involvement adhesion, response. strongly associated modification. knockdown inhibited migration, tumorigenicity, causing G0/G1 phase cycle arrest, expression. These findings may suggest that positively regulates progression of modulates activity making it valuable for treatment prognosis. potential diagnostic marker LUAD, microenvironment, pathway, modification, glycolysis.

Language: Английский

Genetic expression in cancer research: Challenges and complexity DOI
Hengrui Liu, Zheng Guo, Panpan Wang

et al.

Gene Reports, Journal Year: 2024, Volume and Issue: unknown, P. 102042 - 102042

Published: Sept. 1, 2024

Language: Английский

Citations

26
Single-cell and bulk RNA sequencing analysis reveals CENPA as a potential biomarker and therapeutic target in cancers DOI Creative Commons
Hengrui Liu,

Miray Karsidag,

Kunwer Chhatwal

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0314745 - e0314745

Published: Jan. 16, 2025

Cancer remains one of the most significant public health challenges worldwide. A widely recognized hallmark cancer is ability to sustain proliferative signaling, which closely tied various cell cycle processes. Centromere Protein (CENPA), a variant standard histone H3, crucial for selective chromosome segregation during cycle. Despite its importance, comprehensive pan-cancer bioinformatic analysis CENPA has not yet been conducted. Data on genomes, transcriptomes, and clinical information were retrieved from publicly accessible databases. We analyzed CENPA's genetic alterations, mRNA expression, functional enrichment, association with stemness, mutations, expression across populations cellular locations, link cycle, impact survival, relationship immune microenvironment. Additionally, prognostic model glioma patients was developed demonstrate potential as biomarker. Furthermore, drugs targeting in cells identified predicted using drug sensitivity correlations protein-ligand docking. exhibited low levels gene mutation cancers. It found be overexpressed nearly all types TCGA, relative normal controls, predominantly located nucleus malignant cells. showed strong particularly biomarker G2 phase. also emerged valuable diagnostic multiple types. In glioma, demonstrated reliable when used alongside other factors. linked Drugs such CD-437, 3-Cl-AHPC, Trametinib, BI-2536, GSK461364 target serves cancers, offering both value.

Language: Английский

Citations

4
Rational Identification of Ritonavir as IL-20 Receptor A Ligand Endowed with Antiproliferative Properties in Breast Cancer Cells DOI Open Access

Valentina Maggisano,

Adriana Gargano, Jessica Maiuolo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1285 - 1285

Published: Feb. 2, 2025

Targeting the tumor microenvironment (TME) is an attractive strategy for developing new drugs with anticancer activity against triple-negative breast cancer (TNBC). Interleukins (ILs) are key players in TME cytokine network promoting progression. Recent studies have highlighted involvement of IL-20 receptor subunit alpha (IL-20RA) signalling several cancers, including BC, which IL-20RA highly expressed, correlating poor prognosis and influencing tumoral characteristics such as proliferation, cell death, invasiveness, activity. Therefore, elucidating role pathway could form basis therapeutic strategies. This study aimed to identify selective bioactive ligands able affect Virtual screening over 310,000 compounds from both DrugBank ZINC15 databases identified four potential hit tested their TNBC vitro lines. Notably, Ritonavir, a well-known Human Immunodeficiency Virus Type 1 (HIV-1) protease inhibitor, significantly inhibited proliferation (about 40% at 50 µM, p < 0.001). preincubation counteracted Ritonavir’s cytostatic effect while knockdown restored Ritonavir-treated cells. In conclusion, these findings demonstrated that Ritonavir reduced through modulation, suggesting its repurposing agent management.

Language: Английский

Citations

1
Identification of molecular targets of Hypericum perforatum in blood for major depressive disorder: a machine-learning pharmacological study DOI Creative Commons
Zewen Xu,

Ayana Meegol Rasteh,

Angela Dong

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Oct. 9, 2024

Major depressive disorder (MDD) is one of the most common psychiatric disorders worldwide. Hypericum perforatum (HP) a traditional herb that has been shown to have antidepressant effects, but its mechanism unclear. This study aims identify molecular targets HP for treatment MDD.

Language: Английский

Citations

7
Pan-Cancer Genetic Analysis of Mitochondrial DNA Repair Gene Set DOI Creative Commons

Angela Dong,

Ayana Meegol Rasteh,

Hengrui Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Abstract Background The mitochondrial DNA repair has gained attention for its potential impact on pan-cancer genetic analysis. This study investigates the clinical relevance of genes: PARP1, 2, PRIMPOL, TP53, MGME1. Methods Using multi-omics profiling data and Gene Set Cancer Analysis (GSCA) with normalized SEM mRNA expression, this research analyzes differential gene mutation, drug correlation. Results TP53 was most commonly mutated mitochondrial-related in cancer, UCS OV having highest mutation rates. CPG mutations linked to lowest survival Breast various subtypes, potentially influenced by genes. ACC shown be high BRCA, USC, LUCS, COAD, showed CNV levels impacting survival. A negative expression-methylation correlation observed weakest KIRC. Mitochondrial genes were Cell cycle_A activation. weak found between immune infiltration Few compounds affected Conclusion Understanding could redefine cancer diagnosis, prognosis, serve as therapeutic biomarkers, altering cell behavior treatment outcomes.

Language: Английский

Citations

5
Investigating POU3F4 in Cancer: Expression Patterns, Prognostic Implications, and Functional Roles in Tumor Immunity DOI Creative Commons
Weiwei Chen, Yaya Ji, Rui Wang

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(1), P. e41587 - e41587

Published: Jan. 1, 2025

Language: Английский

Citations

0
Integration of machine learning and experimental validation to identify the prognostic signature related to diverse programmed cell deaths in breast cancer DOI Creative Commons

Longpeng Li,

Jinfeng Zhao, Yaxin Wang

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 6, 2025

Programmed cell death (PCD) is closely related to the occurrence, development, and treatment of breast cancer. The aim this study was investigate association between various programmed patterns prognosis cancer (BRCA) patients. levels 19 different deaths in were assessed by ssGSEA analysis, these PCD scores summed obtain PCDS for each sample. relationship with immune as well metabolism-related pathways explored. PCD-associated subtypes obtained unsupervised consensus clustering differentially expressed genes analyzed. prognostic signature (PCDRS) constructed best combination 101 machine learning algorithm combinations, C-index PCDRS compared 30 published signatures. In addition, we analyzed relation therapeutic responses. distribution cells explored single-cell analysis spatial transcriptome analysis. Potential drugs targeting key Cmap. Finally, expression clinical tissues verified RT-PCR. showed higher normal. Different groups significant differences pathways. PCDRS, consisting seven genes, robust predictive ability over other signatures datasets. high group had a poorer strongly associated cancer-promoting tumor microenvironment. low exhibited anti-cancer immunity responded better checkpoint inhibitors chemotherapy-related drugs. Clofibrate imatinib could serve potential small-molecule complexes SLC7A5 BCL2A1, respectively. mRNA upregulated tissues. can be used biomarker assess response BRCA patients, which offers novel insights monitoring personalization

Language: Английский

Citations

0
IL-8 Downregulation Mediates the Beneficial Effects of Infection-Induced Fever on Breast Cancer Prognosis DOI Creative Commons
Heliang Li, Xinyan Liang, Jiafan Ma

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 405 - 419

Published: Jan. 1, 2025

Purpose: Previous studies have reported that infection-induced fever is associated with improved breast cancer prognosis, potentially through the modulation of cytokines. However, key cytokines and underlying mechanisms which exerts its anti-tumor effects remain unclear. Patients Methods: A total 794 patients were recruited between 2008 2017, follow-up extending until October 31st, 2023. Infection-induced was assessed using questionnaires, while a multiplex assay evaluated panel 27 The mediation various analyzed model-based causal analysis. Additionally, we explored modifications to these effect by examining interactions among themselves as well their fever. Bioinformatic analyses conducted elucidate biological pathways mediating Results: relationship prognosis mediated decrease in interleukin-8 (IL-8) levels. Furthermore, our findings revealed downregulation IL-8, mediates beneficial fever, antagonized IL-2, IL12p70 IL-7. By intersecting influenced alongside those affected IL12p70, or IL-7, found latter IL-8 via regulating critical such neutrophil degranulation, extracellular matrix organization asparagine N-linked glycosylation. Conclusion: may improve be involved Such not only provide valuable insights into effectively managing febrile responses for patients, but also underscore therapeutic potential patients. Keywords: cancer, cytokines,

Language: Английский

Citations

0
Heterogeneous nuclear ribonucleoprotein C promotes non-small cell lung cancer progression by enhancing XB130 mRNA stability and translation DOI Creative Commons
Qinrong Wang,

Xuanjing Gou,

Lingling Liu

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 13, 2025

XB130, a classical adaptor protein, exerts critical role in diverse cellular processes. Aberrant expression of XB130 is closely associated with tumorigenesis and aggressiveness. However, the mechanisms governing its regulation remain poorly understood. Heterogeneous nuclear ribonucleoprotein C (hnRNPC), as an RNA-binding known to modulate multiple aspects RNA metabolism has been implicated pathogenesis various cancers. We have previously discovered that hnRNPC one candidate proteins interact 3' untranslated region (3'UTR) non-small cell lung cancer (NSCLC). Therefore, this study aims comprehensively elucidate how regulates NSCLC. evaluated assessed correlation between prognosis patients using public databases. Subsequently, several stable lines were constructed. The proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) these cells detected through Real-time analysis, adherent colony formation, wound healing assay, invasion Western blotting. specific regulatory manner was investigated by quantitative PCR, blotting, pull‑down dual‑luciferase reporter immunoprecipitation, Co-Immunoprecipitation. identified significantly elevated NSCLC correlates poor adenocarcinoma. HnRNPC overexpression increased subsequently activating PI3K/Akt signaling pathway ultimately promoting proliferation EMT. Additionally, overexpressing hnRNPC-silenced partially restored Mechanistically, specifically bound 3'UTR segments mRNA, enhancing mRNA stability inhibiting recruitment nucleases 5'-3' exoribonuclease 1 (XRN1) DIS3-like 3'-5' 2 (DIS3L2). Furthermore, simultaneously interacted eukaryotic initiation factor 4E (eIF4E), component eIF4F complex, facilitating circularization thereby increasing translation efficiency. promotes progression translation, suggesting might be potential therapeutic prognostic target for

Language: Английский

Citations

0
The Role of Osteoprotegerin in Breast Cancer: Genetic Variations, Tumorigenic Pathways, and Therapeutic Potential DOI Open Access
Janan Husain Radhi, Ahmed Mohsen Abbas El-Hagrasy, Sayed Husain Almosawi

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(3), P. 337 - 337

Published: Jan. 21, 2025

Introduction: Osteoprotegerin (OPG), encoded by the TNFRSF11B gene, is linked to development of breast cancer via several pathways, including interactions with receptor activator nuclear factor-κB (RANK) ligands, apoptosis-inducing proteins like TRAIL, and genetic variations such as single nucleotide polymorphisms (SNPs), directly altering gene expression. This review aims investigate role OPG expression in cancer. Methods: A comprehensive literature search was conducted using PubMed Medline, Google Scholar, ScienceDirect. Only full-text English publications from inception September 2024 were included. Results: Studies have demonstrated that certain SNPs specifically rs3102735 rs2073618, are a higher risk development. Additionally, OPG’s function TRAIL decoy may inhibit death cells. Furthermore, serum its BRCA mutations being investigated for their potential influence on progression. found promotes tumorigenesis enhancing cell proliferation, angiogenesis, aneuploidy normal mammary epithelial Moreover, mediates tumor-promoting effects interleukin-1 beta serve biomarker risk, particularly BRCA1 mutation carriers, through dysregulated RANK signaling. Lastly, use recombinant mouse models has been exert anti-tumor effects. Conclusions: In this review, examined. multifaceted exerts TNF-related ligand (TRAIL), modulation pro-tumorigenic microenvironment survival, metastasis. dual tumor suppressor promoter serves possible therapeutic target enhance apoptosis, limit bone metastasis, modulate microenvironment. Whilst much now known, further studies necessary fully delineate OPG.

Language: Английский

Citations

0