Therapeutic peptides: current applications and future directions

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: Feb. 14, 2022

Abstract Peptide drug development has made great progress in the last decade thanks to new production, modification, and analytic technologies. Peptides have been produced modified using both chemical biological methods, together with novel design delivery strategies, which helped overcome inherent drawbacks of peptides allowed continued advancement this field. A wide variety natural obtained studied, covering multiple therapeutic areas. This review summarizes efforts achievements peptide discovery, their current applications. We also discuss value challenges associated future developments peptides.

Language: Английский

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Biocatalysis: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(1), P. 88 - 119

Published: June 18, 2020

Abstract Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes organic synthesis, especially make chiral compounds for pharmaceuticals well the flavors and fragrance industry, are most prominent examples. In addition, biocatalysts used on a large scale specialty even bulk chemicals. This review intends give illustrative examples this field with special focus scalable production using enzymes. It also discusses opportunities limitations enzymatic syntheses distinct provides outlook emerging enzyme classes.

Language: Английский

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Directed Evolution: Methodologies and Applications

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(20), P. 12384 - 12444

Published: July 23, 2021

Directed evolution aims to expedite the natural process of biological molecules and systems in a test tube through iterative rounds gene diversifications library screening/selection. It has become one most powerful widespread tools for engineering improved or novel functions proteins, metabolic pathways, even whole genomes. This review describes commonly used diversification strategies, screening/selection methods, recently developed continuous strategies directed evolution. Moreover, we highlight some representative applications nucleic acids, genetic circuits, viruses, cells. Finally, discuss challenges future perspectives

Language: Английский

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Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(15), P. 6251 - 6290

Published: Jan. 1, 2022

Enzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use technology to improve enzyme performance, still today we are far from full exploitation of field. One clear reason is that evaluate based on only a few experiments not always well-designed. In contrast other reviews subject, here highlight pitfalls using incorrectly designed protocols explain why in cases sub-optimal results obtained. We also describe solutions overcome these challenges come conclusion recent developments material science, bioprocess engineering protein science continue open new opportunities for future. this way, immobilization, being mature discipline, remains as subject high interest where intense research necessary take advantage possibilities.

Language: Английский

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Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 12, 2022

Regenerable nanozymes with high catalytic stability and sustainability are promising substitutes for naturally-occurring enzymes but limited by insufficient non-selective activities. Herein, we developed single-atom of RhN

Language: Английский

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Boron catalysis in a designer enzyme

Nature, Journal Year: 2024, Volume and Issue: 629(8013), P. 824 - 829

Published: May 8, 2024

Language: Английский

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Noncanonical Amino Acids in Biocatalysis

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(14), P. 8740 - 8786

Published: July 3, 2024

In recent years, powerful genetic code reprogramming methods have emerged that allow new functional components to be embedded into proteins as noncanonical amino acid (ncAA) side chains. this review, we will illustrate how the availability of an expanded set building blocks has opened a wealth opportunities in enzymology and biocatalysis research. Genetic provided insights enzyme mechanisms by allowing introduction spectroscopic probes targeted replacement individual atoms or groups. NcAAs also been used develop engineered biocatalysts with improved activity, selectivity, stability, well enzymes artificial regulatory elements are responsive external stimuli. Perhaps most ambitiously, combination laboratory evolution given rise classes use ncAAs key catalytic elements. With framework for developing ncAA-containing now firmly established, optimistic become progressively more tool armory designers engineers coming years.

Language: Английский

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Building Synthetic Cells─From the Technology Infrastructure to Cellular Entities

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(4), P. 974 - 997

Published: March 26, 2024

The de novo construction of a living organism is compelling vision. Despite the astonishing technologies developed to modify cells, building functioning cell "from scratch" has yet be accomplished. pursuit this goal alone has─and will─yield scientific insights affecting fields as diverse biology, biotechnology, medicine, and astrobiology. Multiple approaches have aimed create biochemical systems manifesting common characteristics life, such compartmentalization, metabolism, replication derived features, evolution, responsiveness stimuli, directed movement. Significant achievements in synthesizing each these criteria been made, individually limited combinations. Here, we review efforts, distinguish different approaches, highlight bottlenecks current research. We look ahead at what work remains accomplished propose "roadmap" with key milestones achieve vision cells from molecular parts.

Language: Английский

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Large-scale production of enzymes for biotechnology uses

Current Opinion in Biotechnology, Journal Year: 2020, Volume and Issue: 69, P. 68 - 76

Published: Dec. 31, 2020

Language: Английский

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Harnessing Conformational Plasticity to Generate Designer Enzymes

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(26), P. 11324 - 11342

Published: June 4, 2020

Recent years have witnessed an explosion of interest in understanding the role conformational dynamics both evolution new enzymatic activities from existing enzymes and facilitating emergence activity de novo on scaffolds that were previously non-catalytic. There are also increasing number examples literature targeted engineering being successfully used to alter enzyme selectivity activity. Despite obvious importance function evolvability, many (although not all) computational design approaches still focus either pure sequence-based or using structures with limited flexibility guide design. However, there exist a wide variety can be (re)purposed introduce as key consideration process. Coupled laboratory more conventional sequence- structure-based approaches, these techniques provide powerful tools for greatly expanding protein toolkit. This Perspective provides overview evolutionary studies dissected novel enzymes, well advances allow one target part Harnessing is paradigm which engineer next generation designer biocatalysts.

Language: Английский

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