New plant protection.,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 27, 2024
Abstract
Milbemycins
are
a
group
of
16‐membered
macrolides
produced
by
the
soil‐dwelling
filamentous
bacteria
Streptomyces
.
Renowned
for
their
potent
acaricidal
and
insecticidal
properties,
combined
with
low
toxicity,
milbemycins
recognized
as
eco‐friendly
biopesticides,
vital
pest
control
sustainable
agricultural
development.
Over
several
decades,
have
been
extensively
investigated,
achieving
significant
progress,
including
advancements
in
biological
activities
(such
mechanisms
toxicity
studies),
biosynthetic
regulatory
mechanisms,
high‐yield
strain
engineering
strategies,
development
milbemycin‐derived
commercial
products
applications.
This
review
discusses
recent
advances,
current
limitations,
ongoing
emerging
efforts
to
overcome
limitations
milbemycin
research.
Finally,
future
research
directions
outlined
superior
milbemycin‐producing
cell
factories
facilitate
widespread
application
field.
Synthetic and Systems Biotechnology,
Journal Year:
2025,
Volume and Issue:
10(2), P. 523 - 531
Published: Feb. 14, 2025
Endusamycin,
a
naturally
occurring
polyether
ionophore
antibiotic,
exhibits
extensive
antitumor
activities.
Despite
its
promising
potential,
the
titer
of
endusamycin
is
significantly
lower
compared
to
widely
used
compounds,
and
no
reports
have
been
published
regarding
overproduction.
In
this
study,
various
metabolic
engineering
strategies
were
performed
enhance
production.
Notably,
deletion
competing
biosynthetic
gene
clusters
(BGCs)
responsible
for
biosynthesis
spore
pigment
meilingmycin-like
compounds
based
on
transcriptome
analysis,
as
well
doubling
BGC,
proved
be
effective.
These
interventions
resulted
in
20
%
69
increase
endusamycin,
respectively.
Furthermore,
systematic
optimization
fermentation
medium
components,
including
carbon
source,
nitrogen
phosphorus
potassium,
contributed
further
endusamycin.
Ultimately,
high-yielding
strain
YC1109
was
developed
through
integration
these
strategies.
The
reached
5469
mg/L
shake-flask
5011
fed-batch
fermentation,
representing
246
original
strain.
This
research
facilitates
drug
development
industrialization
It
establishes
superior
chassis
exploring
derivatives
provides
valuable
insights
into
improving
production
compounds.
Synthetic and Systems Biotechnology,
Journal Year:
2025,
Volume and Issue:
10(2), P. 583 - 592
Published: Feb. 20, 2025
Microorganisms
are
often
likened
to
complex
production
workshops.
In
Saccharopolyspora
spinosa
(S.
spinosa),
the
biosynthesis
of
spinosad
is
a
line
within
its
intricate
workshop.
Optimizing
entire
environment
and
reducing
unnecessary
metabolic
flow
essential
increasing
yield.
Pyruvate
serves
as
crucial
precursor
for
biosynthesis.
Previous
studies
revealed
that
pyc
gene
highly
expressed
in
gluconeogenic
pathway,
leading
pyruvate
shunt.
By
downregulating
pyc,
we
enhanced
yield,
although
improvement
was
below
expectations.
We
speculated
most
accumulated
following
knockdown
entered
some
synthetic
pathways
unrelated
spinosad.
Through
pathway
qRT-PCR
analyses,
found
expression
levels
gltA1
atoB3
tributary,
including
TCA
cycle
ethylmalonyl-CoA
were
significantly
increased
strain.
The
combined
these
three
genes
optimized
line,
yield
633.1
±
38.6
mg/L,
representing
199.4
%
increase.
This
study
identifies
key
optimizing
offers
insights
into
screening
efficient
construction
Spinosad-producing
strains.
ACS Synthetic Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 7, 2025
Malonic
acid
(MA)
is
a
high-value
chemical
with
diverse
applications
in
the
fields
of
food,
agriculture,
medicine,
and
synthesis.
Despite
successful
biosynthesis
MA
has
been
performed
Escherichia
coli,
Myceliophthora
thermophila,
Saccharomyces
cerevisiae,
resulting
titers
remain
insufficient
for
industrial-scale
production.
In
this
study,
three
distinct
metabolic
pathways
were
designed
constructed
to
increase
production
E.
coli.
Among
these,
fumaric
pathway
comprising
four
key
enzymes
including
aspartase
(AspA),
decarboxylase
(PanD),
β-alanine-pyruvate
transaminase
(Pa0132),
succinic
aldehyde
dehydrogenase
(YneI)
was
identified
as
most
effective
Additionally,
supplementation
found
significantly
improve
To
further
enhance
production,
engineering
strategies
employed,
deletion
ydfG
gene,
responsible
encoding
malonic
semialdehyde
reductase,
ptsG
which
encodes
glucose
transporter.
Finally,
through
optimization
fermentation
conditions
feeding
strategies,
engineered
strain
achieved
an
titer
1.4
g/L
shake
flask
17.8
fed-batch
fermentation.
This
study
provides
new
insights
into
utilizing
metabolically
coli
cells.
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 16, 2025
Docosapentaenoic
acid
(DPA)
is
widely
applied
in
medicine
and
health
products
because
of
its
important
physiological
functions.
Using
microbial
cell
factories
for
DPA
production
considered
a
viable
alternative
to
extracting
from
seal
oil.
In
this
study,
an
engineering
strategy
the
efficient
was
developed.
First,
biosynthesis
pathway
successfully
established
Yarrowia
lipolytica.
Then,
increase
acetyl-CoA
by
citrate
metabolism
malonyl-CoA
introducing
new
orthogonal
synthesis
further
enhance
production.
Furthermore,
overexpression
glucose-6-phosphate
dehydrogenase
(G6PDH)
malic
enzyme
(ME)
enhanced
NADPH
availability.
Finally,
optimizing
fermentation
conditions,
content
engineered
strain
reached
40.0%,
yield
13.0
g/L
5
L
bioreactor,
representing
highest
levels
reported
so
far
Y.
This
study
provides
promising
construct
fatty
biosynthesis.
Microbial Biotechnology,
Journal Year:
2024,
Volume and Issue:
17(11)
Published: Nov. 1, 2024
Abstract
Malonyl‐coenzyme
A
(CoA)
is
a
key
precursor
for
the
biosynthesis
of
multiple
value‐added
compounds
by
microbial
cell
factories,
including
polyketides,
carboxylic
acids,
biofuels,
and
polyhydroxyalkanoates.
Owing
to
its
role
as
metabolic
hub,
malonyl‐CoA
availability
limited
competition
in
several
essential
pathways.
To
address
this
limitation,
we
modified
genome‐reduced
Pseudomonas
putida
strain
increase
acetyl‐CoA
carboxylation
while
limiting
utilization.
Genes
involved
sugar
catabolism
regulation,
tricarboxylic
acid
(TCA)
cycle,
fatty
were
knocked‐out
specific
combinations
towards
increasing
pool.
An
enzyme‐coupled
biosensor,
based
on
rppA
gene,
was
employed
monitor
levels
vivo.
RppA
type
III
polyketide
synthase
that
converts
into
flaviolin,
red‐colored
polyketide.
We
isolated
strains
displaying
enhanced
via
colorimetric
screening
method
RppA‐dependent
red
pigmentation;
direct
flaviolin
quantification
identified
four
engineered
had
significant
levels.
further
these
adding
non‐canonical
pathway
uses
poly(3‐hydroxybutyrate)
biosynthesis.
These
manipulations
led
increased
polymer
accumulation
fully
strains,
validating
our
general
strategy
boost
output
malonyl‐CoA–dependent
pathways
P
.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(5), P. 1132 - 1132
Published: March 3, 2024
During
the
life
activities
of
microorganisms,
a
variety
secondary
metabolites
are
produced,
including
antimicrobials
and
antitumor
drugs,
which
widely
used
in
clinical
practice.
In
addition
to
exploring
new
antibiotics,
this
makes
it
one
research
priorities
Actinomycetes
effectively
increase
yield
antibiotics
production
strains
by
various
means.
Most
antibiotic-producing
have
functional
regulatory
factors
that
regulate
their
growth,
development,
metabolite
biosynthesis
processes.
Through
study
precursor
substances
antibiotic
biosynthesis,
researchers
revealed
process
mechanism
synthesis
regulators
affect
metabolites,
can
be
obtain
engineered
with
high
production.
This
paper
summarizes
supply
precursors
progress
on
role
biosynthesis.
lays
foundation
for
establishment
effective
breeding
methods
improve
yields
through
manipulation
genes
related
regulators.
