Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors DOI Creative Commons
Dirk Schenke, Daguang Cai

iScience, Journal Year: 2020, Volume and Issue: 23(9), P. 101478 - 101478

Published: Aug. 20, 2020

Current crop production systems are prone to increasing pathogen pressure. Fundamental understanding of molecular plant-pathogen interactions, the availability and genomic information, as well emerging genome editing permits a novel approach for breeding disease resistance. We describe here strategies identify new targets resistance with focus on interruption compatible interaction by CRISPR/Cas-mediated editing. Basically, can be applied in several ways achieve this goal. The most common focuses "simple" knockout non-homologous end joining repair plant susceptibility factors required efficient host colonization. However, re-writing via homology-directed or base also prevent manipulation changing pathogen-derived effectors molecules beyond recognition, which decreases susceptibility. conclude that CRISPR/Cas will become increasingly indispensable generate relatively short time beneficial traits crops meet upcoming challenges.

Language: Английский

Strategies for mitigation of pesticides from the environment through alternative approaches: A review of recent developments and future prospects DOI
Veena Chaudhary, Mukesh Kumar, Chetan Chauhan

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 354, P. 120326 - 120326

Published: Feb. 21, 2024

Language: Английский

Citations

16
CRISPR/Cas9 for development of disease resistance in plants: recent progress, limitations and future prospects DOI
Shakeel Ahmad, Xiangjin Wei,

Zhonghua Sheng

et al.

Briefings in Functional Genomics, Journal Year: 2019, Volume and Issue: 19(1), P. 26 - 39

Published: Dec. 18, 2019

Several plant pathogens severely affect crop yield and quality, thereby threatening global food security. In order to cope with this challenge, genetic improvement of disease resistance is required for sustainable agricultural production, which conventional breeding unlikely do enough. Luckily, genome editing systems that particularly clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) has revolutionized by enabling robust precise targeted modifications. It paves the way towards new methods accelerates breeding. review, challenges, limitations prospects applications CRISPR/Cas9 system development transgene-free disease-resistant crops are discussed.

Language: Английский

Citations

125
Virus Latency and the Impact on Plants DOI Creative Commons
Hideki Takahashi, Toshiyuki Fukuhara, Haruki Kitazawa

et al.

Frontiers in Microbiology, Journal Year: 2019, Volume and Issue: 10

Published: Dec. 6, 2019

Plant viruses are thought to be essentially harmful the lives of their cultivated crop hosts. In most cases studied, interaction between and plants negatively affects host morphology physiology, thereby resulting in disease. Native wild/non-cultivated often latently infected with without any clear symptoms. Although seemingly non-harmful, these pose a threat crops because they can transmitted by vectors cause Reports accumulating on infections latent plant that do not disease but rather seem beneficial wild plants. few cases, viral latency involves integration full-length genome copies into that, response environmental stress or during certain developmental stages plants, become activated generate replicate episomal copies, transition from reactivation causation development. The may also lead partial-length segments DNA genomes copy RNA sequences genome. Transcripts derived such integrated elements (EVEs) for example, conferring levels virus resistance and/or causing persistence/latency infections. Studies might help us understand elucidate underlying mechanisms provide insights raison d'être

Language: Английский

Citations

120
Mutation Breeding in Tomato: Advances, Applicability and Challenges DOI Creative Commons

Juhi Chaudhary,

Alisha Alisha,

Vacha Bhatt

et al.

Plants, Journal Year: 2019, Volume and Issue: 8(5), P. 128 - 128

Published: May 14, 2019

Induced mutagenesis is one of the most effective strategies for trait improvement without altering well-optimized genetic background cultivars. In this review, several currently accessible methods such as physical, chemical and insertional have been discussed concerning their efficient exploration tomato crop improvement. Similarly, challenges adaptation genome-editing, a newly developed technique providing an opportunity to induce precise mutation, addressed. Several efforts genome-editing demonstrated in other crops, exploring its effectiveness convenience Descriptive data compiled here from will be helpful technological advances. However, uncertainty about regulation genome-edited crops still significant concern, particularly when timely cultivars needed. regard, random approaches induced are promising if efficiently explored breeding applications. Precise identification casual mutation prerequisite molecular understanding development well utilization program. Recent advances sequencing techniques provide detection mutagenesis-induced sequence variations at large scale genome. Here, we reviewed novel next-generation based mapping including Mutmap, MutChromeSeq, whole-genome sequencing-based which has enormous potential accelerate tomato. The proper existing well-characterized mutant resources combined with would inevitably lead rapid enhancement quality yield. This article provides overview principles applications discusses current progress involved research.

Language: Английский

Citations

113
Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era DOI Creative Commons
Rahul Mahadev Shelake, Dibyajyoti Pramanik, Jae‐Yean Kim

et al.

Microorganisms, Journal Year: 2019, Volume and Issue: 7(8), P. 269 - 269

Published: Aug. 17, 2019

Plants and microbes are co-evolved interact with each other in nature. Plant-associated microbes, often referred to as plant microbiota, an integral part of life. Depending on the health effects hosts, plant–microbe (PM) interactions either beneficial or harmful. The role microbiota growth promotion (PGP) protection against various stresses is well known. Recently, our knowledge community composition microbiome significant driving factors have significantly improved. So, use a reliable approach for next green revolution meet global food demand sustainable eco-friendly agriculture. An application multifaceted PM needs novel tools know critical genetic molecular aspects. Recently discovered clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-mediated genome editing (GE) great interest explore interactions. A systematic understanding will enable GE enhance capacity plants agronomic trait improvement. This review focuses applying techniques associated discovering fundamentals interactions, disease resistance, PGP activity, future implications

Language: Английский

Citations

112
Review of empirical and emerging breeding methods and tools for yam (Dioscorea spp.) improvement: Status and prospects DOI Creative Commons
Kwabena Darkwa, Bunmi Olasanmi, Robert Asiedu

et al.

Plant Breeding, Journal Year: 2019, Volume and Issue: 139(3), P. 474 - 497

Published: Dec. 5, 2019

Abstract Yam ( Dioscorea spp.) is an important tuber crop with tremendous potential as a functional food in the tropics and subtropics. However, has not shown progressive productivity gain over decades due to various production constraints. This paper reviews progress achieved empirical breeding endeavours development, status application of emerging tools technologies translate genetic gains yam improvement. Significant been made improvement years which led identification development several improved clones sources variability for economically traits. Substantial efforts have also develop diverse molecular markers, transcriptome metabolome profiles crucial traits, trait mapping generate reference genome sequences key species. there seems be slow translation research improvements into widespread applications. These advances integration methods process will enhance ensure quick delivery varieties that possess superior agronomic quality

Language: Английский

Citations

109
Genome editing for horticultural crop improvement DOI Creative Commons
Jiemeng Xu, Kai Hua,

Zhaobo Lang

et al.

Horticulture Research, Journal Year: 2019, Volume and Issue: 6(1)

Published: Oct. 7, 2019

Horticultural crops provide humans with many valuable products. The improvement of the yield and quality horticultural has been receiving increasing research attention. Given development advantages genome-editing technologies, that uses genome editing to improve substantially increased in recent years. Here, we briefly review different systems used a focus on clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-mediated editing. We also summarize progress application for crop improvement. combination rapidly advancing technology breeding will greatly increase production quality.

Language: Английский

Citations

106
Application of Gene Editing for Climate Change in Agriculture DOI Creative Commons
Nicholas G. Karavolias,

Wilson Horner,

Modesta Abugu

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2021, Volume and Issue: 5

Published: Sept. 7, 2021

Climate change imposes a severe threat to agricultural systems, food security, and human nutrition. Meanwhile, efforts in crop livestock gene editing have been undertaken improve performance across range of traits. Many the targeted phenotypes include attributes that could be beneficial for climate adaptation. Here, we present examples emerging applications research initiatives are aimed at improvement crops response change, discuss technical limitations opportunities therein. While only few translated production thus far, numerous studies settings demonstrated potential potent address near future.

Language: Английский

Citations

101
CRISPR/Cas9-based genome editing of banana for disease resistance DOI
Leena Tripathi, Valentine Otang Ntui, Jaindra Nath Tripathi

et al.

Current Opinion in Plant Biology, Journal Year: 2020, Volume and Issue: 56, P. 118 - 126

Published: June 27, 2020

Language: Английский

Citations

96
Harnessing Genome Editing Techniques to Engineer Disease Resistance in Plants DOI Creative Commons

Muntazir Mushtaq,

Aafreen Sakina,

Shabir Hussain Wani

et al.

Frontiers in Plant Science, Journal Year: 2019, Volume and Issue: 10

Published: May 7, 2019

Modern genome editing (GE) techniques, which include clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system, transcription activator-like effector nucleases (TALENs), zinc-finger (ZFNs) and LAGLIDADG homing endonucleases (meganucleases), have so far been used for engineering disease resistance in crops. . The use of GE technologies has grown very rapidly recent years with numerous examples targeted mutagenesis crop plants, including gene knockouts, knockdowns, modifications, the repression activation target genes. CRISPR/Cas9 supersedes all other techniques TALENs ZFNs genes owing to its unprecedented efficiency, relative simplicity low risk off-target effects Broad-spectrum engineered crops by either specific host-susceptibility (S approach), or cleaving DNA phytopathogens (bacteria, virus fungi) inhibit their proliferation. This review focuses on different that can potentially be boost molecular immunity against crops, ultimately leading development promising disease-resistant varieties.

Language: Английский

Citations

83