The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late gestation DOI Creative Commons
Victor A. Ruthig, Talia Hatkevich,

Josiah Hardy

et al.

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(3), P. e1010656 - e1010656

Published: March 1, 2023

DND1 is essential to maintain germ cell identity. Loss of Dnd1 function results in differentiation teratomas some inbred strains mice or somatic fates zebrafish. Using our knock-in mouse line which a functional fusion protein between and GFP expressed from the endogenous locus ( ), we distinguished two male (MGC) populations during late gestation cycle arrest (G0), consistent with recent reports heterogeneity among MGCs. Most MGCs express lower levels DND1-GFP (DND1-GFP-lo), but elevated (DND1-GFP-hi). A RNA-seq time course confirmed high transcript DND1-GFP-hi cells along 5-10-fold higher for multiple epigenetic regulators. antibodies against RNA immunoprecipitation (RIP)-sequencing, identified translational regulators that are binding targets G0 including DNA methyltransferases (Dnmts), histone deacetylases (Hdacs), Tudor domain proteins (Tdrds), actin dependent (Smarcs), group ribosomal Golgi proteins. These data suggest cells, hosts coordinating mRNA regulons consist functionally related localized groups enzymes components.

Language: Английский

Piezo1 regulates the regenerative capacity of skeletal muscles via orchestration of stem cell morphological states DOI Creative Commons
Nuoying Ma, Delia Chen, Jihyung Lee

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(11)

Published: March 18, 2022

Muscle stem cells (MuSCs) are essential for tissue homeostasis and regeneration, but the potential contribution of MuSC morphology to in vivo function remains unknown. Here, we demonstrate that quiescent MuSCs morphologically heterogeneous exhibit different patterns cellular protrusions. We classified into three functionally distinct cell states: responsive, intermediate, sensory. shift between states promotes regeneration is regulated by sensing protein Piezo1. Pharmacological activation Piezo1 sufficient prime toward more responsive cells. deletion shifts distribution less cells, mimicking disease phenotype find dystrophic muscles. further reactivation ameliorates morphological regenerative defects These findings advance our fundamental understanding how respond injury identify as a key regulator adjusting regeneration.

Language: Английский

Citations

81
An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling DOI Creative Commons
Allison P Kann, Margaret Hung, Wei Wang

et al.

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(6), P. 933 - 947.e6

Published: May 20, 2022

Language: Английский

Citations

58
The MicroRNA miR-277 Controls Physiology and Pathology of the Adult Drosophila Midgut by Regulating the Expression of Fatty Acid β-Oxidation-Related Genes in Intestinal Stem Cells DOI Creative Commons
Lisa Zipper, Sai Batchu, Nida Hatice Kaya

et al.

Metabolites, Journal Year: 2022, Volume and Issue: 12(4), P. 315 - 315

Published: March 31, 2022

Cell division, growth, and differentiation are energetically costly dependent processes. In adult stem cell-based epithelia, cellular identity seems to be coupled with a cell's metabolic profile vice versa. It is thus tempting speculate that resident cells have distinct metabolism, different from more committed progenitors differentiated cells. Although investigated for many cell types in vitro, vivo data of niche-residing metabolism scarce. epithelial tissues, cells, progenitor their progeny very functions characteristics. our study, we hypothesized tested whether might distinctive the intestinal lineage. Here, taking advantage genetically accessible

Language: Английский

Citations

11
Quiescent Adult Neural Stem Cells: Developmental Origin and Regulatory Mechanisms DOI Creative Commons
Meng Han, Huan Yu, Kun Zhang

et al.

Neuroscience Bulletin, Journal Year: 2024, Volume and Issue: 40(9), P. 1353 - 1363

Published: April 24, 2024

The existence of neural stem cells (NSCs) in the adult mammalian nervous system, although small number and restricted to sub-ventricular zone lateral ventricles, dentate gyrus hippocampus, olfactory epithelium, is a gift evolution for adaptive brain function which requires persistent plastic changes these regions. It known that most NSCs are latent, showing long cell cycles. In past decade, concept quiescent (qNSCs) has been widely accepted by researchers field, great progress made biology qNSCs. Although spontaneous neuronal regeneration derived from not significant, understanding how behaviors qNSCs regulated sheds light on stimulating endogenous NSC-based regeneration. this review, we mainly focus recent developmental origin regulatory mechanisms maintain under normal conditions, mobilize pathological hoping give some insights future study.

Language: Английский

Citations

2
Mitochondrial Control of Stem Cell State and Fate: Lessons From Drosophila DOI Creative Commons
Satish Kumar Tiwari, Sudip Mandal

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: May 3, 2021

Over the years, Drosophila has served as a wonderful genetically tractable model system to unravel various facets of tissue-resident stem cells in their microenvironment. Studies different and progenitor cell types have led discovery cell-intrinsic extrinsic factors crucial for state fate. Though initially touted ATP generating machines carrying cellular processes, it is now increasingly becoming clear that mitochondrial processes alone can override program cells. The last few years witnessed surge our understanding mitochondria’s contribution governing properties subtissular niches . Through this review, we intend sum up highlight outcome these vivo studies implicate mitochondria central regulator fate decisions; find commonalities uniqueness associated with regulatory mechanisms.

Language: Английский

Citations

12
Male-specific sNPF peptidergic circuits control energy balance for mating duration through neuron-glia interactions. DOI Open Access
Xiaoli Zhang, Hongyu Miao, Dayoon Kang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

ABSTRACT This study reveals a sexually dimorphic sNPF-sNPF-R circuit in the Drosophila brain that regulates energy balance behavior, specifically shorter-mating-duration (SMD) response to sexual experience. sNPF is predominantly expressed neurons, while sNPF-R both neurons and glial cells, particularly astrocyte-like glia (ALG) subset of cells outside mushroom body (MB) termed "Rishi cells" (RS cells). Sexual experience induces global alterations calcium signaling synaptic plasticity within this circuit, with expressing RS playing critical role encoding experience-related information into behavior related mating duration. Neuronal glucose metabolism, Tret1l transporter, essential for maintaining high levels proper function near cells. novel intricate neuron-glia network acts as circuits processing interval timing behaviors, highlighting dynamic interplay between neural circuits, regulating balance.

Language: Английский

Citations

1
Mitochondrial respiration and dynamics of in vivo neural stem cells DOI Creative Commons
Stavroula Petridi, Dnyanesh Dubal, Richa Rikhy

et al.

Development, Journal Year: 2022, Volume and Issue: 149(23)

Published: Nov. 29, 2022

ABSTRACT Neural stem cells (NSCs) in the developing and adult brain undergo many different transitions, tightly regulated by extrinsic intrinsic factors. While role of signalling pathways transcription factors is well established, recent evidence has also highlighted mitochondria as central players NSC behaviour fate decisions. Many aspects cellular metabolism mitochondrial biology change during interact with affect activity chromatin-modifying enzymes. In this Spotlight, we explore vivo findings, primarily from Drosophila mammalian model systems, about that respiration morphology play development function.

Language: Английский

Citations

3
The RNA binding protein DND1 is elevated in a subpopulation of pro-spermatogonia and targets chromatin modifiers and translational machinery during late gestation DOI Creative Commons
Victor A. Ruthig, Talia Hatkevich,

Josiah Hardy

et al.

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(3), P. e1010656 - e1010656

Published: March 1, 2023

DND1 is essential to maintain germ cell identity. Loss of Dnd1 function results in differentiation teratomas some inbred strains mice or somatic fates zebrafish. Using our knock-in mouse line which a functional fusion protein between and GFP expressed from the endogenous locus ( ), we distinguished two male (MGC) populations during late gestation cycle arrest (G0), consistent with recent reports heterogeneity among MGCs. Most MGCs express lower levels DND1-GFP (DND1-GFP-lo), but elevated (DND1-GFP-hi). A RNA-seq time course confirmed high transcript DND1-GFP-hi cells along 5-10-fold higher for multiple epigenetic regulators. antibodies against RNA immunoprecipitation (RIP)-sequencing, identified translational regulators that are binding targets G0 including DNA methyltransferases (Dnmts), histone deacetylases (Hdacs), Tudor domain proteins (Tdrds), actin dependent (Smarcs), group ribosomal Golgi proteins. These data suggest cells, hosts coordinating mRNA regulons consist functionally related localized groups enzymes components.

Language: Английский

Citations

1