The chemosensory protein 1 contributes to indoxacarb resistance in Plutella xylostella(L.) DOI
Yifan Li,

Shujun Ni,

Yunping Wang

et al.

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(7), P. 2456 - 2468

Published: Feb. 21, 2023

Insecticide resistance continuously poses a threat to agricultural production. Chemosensory protein-mediated is new mechanism of insecticide discovered in recent years. In-depth research on mediated by chemosensory proteins (CSPs) provides insight into aid management.Chemosensory protein 1 Plutella xylostella (PxCSP1) was overexpressed the two indoxacarb-resistant field populations and PxCSP1 has high affinity with indoxacarb. upregulated when exposed indoxacarb knockdown this gene elevated sensitivity indoxacarb, which demonstrate that involved resistance. Considering CSPs may confer insects via binding or sequestering, we explored PxCSP1-mediated Using molecular dynamics simulations site-directed mutation, found forms solid complex mainly through van der Waals interactions electrostatic interactions. Between these, interaction provided Lys100 side chain PxCSP1, especially hydrogen bonding between NZ atom O carbamoyl carbonyl group are key factors for indoxacarb.The overexpression PxCPS1 its partially responsible P. xylostella. Modification indoxacarb's potential alleviate These findings will contribute solving provide better understanding mechanism. © 2023 Society Chemical Industry.

Language: Английский

Molecular innovations underlying resistance to nicotine and neonicotinoids in the aphid Myzus persicae DOI
Bartlomiej J. Troczka, Kumar Saurabh Singh, Christoph T. Zimmer

et al.

Pest Management Science, Journal Year: 2021, Volume and Issue: 77(12), P. 5311 - 5320

Published: July 16, 2021

The green peach aphid, Myzus persicae, is a globally distributed highly damaging crop pest. This species has demonstrated an exceptional ability to evolve resistance both synthetic insecticides used for control, and natural produced by certain plants as chemical defense against insect attack. Here we review work characterizing the evolution of in M. persicae insecticide nicotine structurally related class neonicotinoid insecticides. We outline how research on this topic provided insights into long-standing questions evolutionary applied importance. These include pertaining origins novel traits, number nature mutational events or 'adaptive steps' underlying new phenotypes, whether host plant adaptations can be co-opted confer Finally, molecular mechanisms generated several outstanding genetic architecture xenobiotics, conclude identifying key knowledge gaps future research. © 2021 Authors. Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

30
Development of Beauveria bassiana (Ascomycota: Hypocreales) as a mycoinsecticide to control green peach aphid, Myzus persicae (Homoptera: Aphididae) and investigation of its biocontrol potential DOI
Seda Biryol, Zihni Demirbağ, Pervin Erdoğan

et al.

Journal of Asia-Pacific Entomology, Journal Year: 2022, Volume and Issue: 25(1), P. 101878 - 101878

Published: Jan. 26, 2022

Language: Английский

Citations

21
Functional investigation of lncRNAs and target cytochrome P450 genes related to spirotetramat resistance in Aphis gossypii Glover DOI
Tianfei Peng, Xuemei Liu,

Tian FaYi

et al.

Pest Management Science, Journal Year: 2022, Volume and Issue: 78(5), P. 1982 - 1991

Published: Jan. 29, 2022

Spirotetramat is a tetramic acid derivative insecticide with novel modes of action for controlling Aphis gossypii Glover in the field. Previous studies have shown that long noncoding RNAs (lncRNAs) and cytochrome P450 monooxygenases (P450s) are involved detoxification process. However, functions lncRNAs regulating gene expression spirotetramat resistance A. unknown.In this study, we found CYP4CJ1, CYP6CY7 CYP6CY21 levels to be significantly upregulated spirotetramat-resistant (SR) strain compared susceptible (SS) strain. Furthermore, knockdown increased nymph adult mortality SR following exposure spirotetramat. Drosophila ectopically expressing CYP380C6, CYP6DA2, showed decreased after exposure, CYP4CJ1 putative targets six lncRNAs. Silencing MSTRG.36649.2/5 MSTRG.71880.1 changed CYP380C6 expression, altering sensitivity Moreover, did not compete microRNA (miRNA) binding regulate expression.Our results confirm potentially development gossypii, probably other than through "sponge effect" competing miRNA binding. Our provide favorable molecular basis studying cotton aphid genes lncRNA development.

Language: Английский

Citations

19
Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest DOI Creative Commons
Ernesto Villacis‐Perez, Wenxin Xue, Marilou Vandenhole

et al.

Evolutionary Applications, Journal Year: 2023, Volume and Issue: 16(4), P. 863 - 879

Published: March 25, 2023

Abstract Pesticide resistance relies on a myriad of mechanisms, ranging from single mutations to complex and polygenic architecture, it involves mechanisms such as target‐site insensitivity, metabolic detoxification, or combination these, with either additive synergistic effects. Several against abamectin, macrocyclic lactone widely used in crop protection, have been reported the cosmopolitan pest Tetranychus urticae . However, has shown that mechanism cannot account for high levels abamectin found across different mite populations. Here, we experimental evolution combined bulked segregant analyses map quantitative trait loci (QTL) associated two genetically unrelated populations T. In these independent QTL mapping experiments, three four QTLs were identified, which shared between experiments. Shared contained genes encoding subunits glutamate‐gated chloride channel (GluCl) harboured previously mutations, including G314D GluCl1 G326E GluCl3 , but also novel candidate loci, DNA helicases chemosensory receptors. Surprisingly, fourth QTL, present only one experiments thus unique resistant parental line, revealed non‐functional variant GluCl2 suggesting gene knock‐out mechanism. Our study uncovers basis resistance, highlights intraspecific diversity genetic underlying pest.

Language: Английский

Citations

11
Resistance to both aphids and nematodes in tobacco plants expressing a Bacillus thuringiensis crystal protein DOI
Yong Wang,

MengNan Wang,

Yali Zhang

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(7), P. 3098 - 3106

Published: Feb. 7, 2024

Bacillus thuringiensis (Bt) and its crystal toxin or δ-endotoxins (Cry) offer great potential for the efficient control of crop pests. A vast number pests can potentially infect same host plant, either simultaneously sequentially. However, no effective Bt-Cry protein has been reported to both aphids plant parasitic nematodes due highly specific activity.

Language: Английский

Citations

4
Myzus persicae resistance to neonicotinoids—unravelling the contribution of different mechanisms to phenotype DOI Creative Commons
Claire Mottet, Laëtitia Caddoux, Séverine Fontaine

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(11), P. 5852 - 5863

Published: July 23, 2024

Abstract Background Deciphering the mechanisms underlying insecticide resistance is key to devising appropriate strategies against this economically important trait. Myzus persicae , green peach‐potato aphid, a major pest that has evolved many classes, including neonicotinoids. M. neonicotinoids previously been shown result from two main mechanisms: metabolic resulting P450 overexpression and targetsite mutation, R81T. However, their respective contribution resistant phenotypes remains unclear. Results By combining extensive bioassays with without addition of synergist PBO, gene copy number expression quantification enzymes ( CYP6CY3 CYP6CY4 ) in 23 clone collection, we, (i) confirmed correlated level, up threshold, (ii) demonstrated R81T homozygous state combination overexpression, leads high levels neonicotinoids, and, (iii) showed there synergistic interaction between mechanisms, strongest impact on strength phenotypes. even though mutation great effect phenotype, different genotypes can exhibit variation level resistance, explained only partially by overexpression. Conclusion To comprehend phenotypes, it take into account every mechanism at play, as well way these interact. © 2024 The Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

4
Selection of Sclerodermus pupariae Reference Genes for Quantitative Real-Time PCR DOI Creative Commons
Ting Zhou,

Huahua Feng,

Jie Zhang

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 268 - 268

Published: March 4, 2025

S. pupariae is a newly discovered species of parasitoid wasps. Research into its development, behavioral genetics, and molecular mechanisms provides valuable insights for improving integrated pest management strategies. Quantitative real-time PCR (qRT-PCR) the most commonly used method analyzing gene expression. This requires identification stable reference genes to accurately evaluate transcriptional level variations. In this study, eight candidate (TUB, TBP, RPS18, GAPDH, 18S rRNA, RPL32, Actin, EF1-α) were identified evaluated their suitability as genes. Gene expression levels across different developmental stages analyzed using three software tools, GeNorm, NormFinder, BestKeeper, online tool RefFinder. The overall ranking stability was follows: RPS18 > rRNA RPL32 GAPDH Actin TUB TPB EF1-α. Ultimately, determined be gene.

Language: Английский

Citations

0
Endosymbionts in agriculturally important parasitoid wasps from Australia DOI Creative Commons

Hareem Qazi,

Qiong Yang, Jing Zhao

et al.

Biological Journal of the Linnean Society, Journal Year: 2025, Volume and Issue: 144(3)

Published: March 1, 2025

Abstract Parasitoid wasps are important biocontrol agents for agricultural pests, with increasing demand their use in sustainable pest management practices. Endosymbiotic bacteria of parasitoid can have a range fitness effects on insect hosts, and these could be manipulated to enhance commercial stocks. Here, we characterized the diversity distribution key endosymbiont species parasitoids that Australian agriculture. In total, screened 12 field-collected aphid parasitoids, 32 lepidopteran 11 commercially available sourced from biological control companies. Our results suggest infections uncommon across different Australia but found high frequencies within some species. Wolbachia was most prevalent reared whitefly scale parasitoids. These all belong Supergroups A B. findings provide useful basis future investigations phenotypic infections, long-term view support effectiveness as management.

Language: Английский

Citations

0
Influence of phloem lectin CsPP2‐A1 on aphid development via mediation of phenylpropanoid and flavonoid biosynthesis in cucumber DOI

Li Yapeng,

Yu Zhu,

Dong Lele

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract BACKGROUND Aphid, Aphis gossypii Glover, is a pest that significantly affects cucumbers ( Cucumis sativus L.). Phloem protein 2 (PP2) conserved phloem lectin. Our previous study showed the expression of CsPP2‐A1 under aphid attack affected accumulation flavonoids and total phenolics in cucumber. The novel mechanism lectin mediating secondary metabolites affecting resistance needs to be investigated. RESULTS weight length aphids on overexpression CsPP2‐A1‐ OE) cucumber plants reduced compared wild‐type (WT). Conversely, RNA interference RNAi) opposite trend. Using metabolomics, small molecular were qualitatively quantitatively assessed WT transgenic after inoculation. resulted up‐regulation differential (DMs) phenylpropanoid biosynthesis, whereas led down‐regulation DMs flavonoid biosynthesis. Concurrently, it was observed CAD activity CsPAL , CsCAD OE‐2 up‐regulated significantly. A significant reduction activities CHI, F3H, CsF3H CsCHS CsFLS CsCCR noted RNAi‐2. CONCLUSION indirectly growth development via mediation indirect effects interaction with offer insights into plant–insect studies. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Potato Leafroll Virus DOI
Tahsin Shoala

Published: Jan. 1, 2025

Citations

0