Independent Genetic Mapping Experiments Identify Diverse Molecular Determinants of Host Adaptation in a Generalist Herbivore DOI
Ernesto Villacis‐Perez, Femke De Graeve, Berdien De Beer

et al.

Molecular Ecology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

ABSTRACT Interactions between plants and herbivores promote evolutionary change. Studying the evolution of herbivore mechanisms aimed to cope with different host plant species is a critical intersection biology sustainable pest management. Generalist are particular interest, as hybridization genetically distinct populations can increase standing genetic variation therefore adaptive potential species. Tetranychus urticae generalist arthropod known for its potential, evidenced in immense range ability develop metabolic resistance xenobiotics. However, molecular underpinnings associated adaptation consequences this, many other pests remain elusive. Here, we use two independent, empirical approaches identify map basis performance T. . In first approach, subject diverse mite population tomato selection genomic regions linked phenotypic increased reproductive performance. second responsible on by comparing genomes pooled individuals from an F2 backcross high low Both revealed specific shared key candidate genes were identified. Our findings highlight power spider complex herbivores.

Language: Английский

A review of the molecular mechanisms of acaricide resistance in mites and ticks DOI Creative Commons
Sander De Rouck, Emre İnak, Wannes Dermauw

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 159, P. 103981 - 103981

Published: June 28, 2023

The Arachnida subclass of Acari comprises many harmful pests that threaten agriculture as well animal health, including herbivorous spider mites, the bee parasite Varroa, poultry mite Dermanyssus and several species ticks. Especially in agriculture, acaricides are often used intensively to minimize damage they inflict, promoting development resistance. Beneficial predatory mites biological control also subjected acaricide selection field. use new genetic genomic tools such genome transcriptome sequencing, bulked segregant analysis (QTL mapping), reverse genetics via RNAi or CRISPR/Cas9, have greatly increased our understanding molecular mechanisms resistance Acari, especially Tetranychus urticae which emerged a model species. These techniques allowed uncover validate mutations larger range In addition, provided an impetus start elucidating more challenging questions on gene regulation detoxification associated with

Language: Английский

Citations

86
SYNCAS: Efficient CRISPR/Cas9 gene-editing in difficult to transform arthropods DOI Creative Commons
Sander De Rouck, Antonio Mocchetti, Wannes Dermauw

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 165, P. 104068 - 104068

Published: Jan. 1, 2024

The genome editing technique CRISPR/Cas9 has led to major advancements in many research fields and this state-of-the-art tool proven its use genetic studies for various arthropods. However, most transformation protocols rely on microinjection of component into embryos, a method which is challenging species. Alternatively, injections can be performed adult females, but efficiencies very low as was shown the two-spotted spider mite, Tetranychus urticae, minute important chelicerate pest crops. In study, we explored different formulations optimize maternal injection protocol T. urticae. We observed strong synergy between branched amphipathic peptide capsules saponins, resulting significant increase knock-out efficiency, exceeding 20%. This formulation, termed SYNCAS, used urticae genes – phytoene desaturase, CYP384A1 Antennapedia also allowed develop co-CRISPR strategy facilitated generation knock-in mutants. addition, SYNCAS successfully applied white white-like western flower thrips, Frankliniella occidentalis. allows routine these species game changer other hard transform

Language: Английский

Citations

19
Cross‐resistance, inheritance and biochemical mechanism of abamectin resistance in a field‐derived strain of the citrus red mite, Panonychus citri (Acari: Tetranychidae) DOI

Xun‐Yan Liu,

Ke Li, Deng Pan

et al.

Pest Management Science, Journal Year: 2023, Volume and Issue: 80(3), P. 1258 - 1265

Published: Oct. 27, 2023

Abstract BACKGROUND The citrus red mite, Panonychus citri (McGregor), a global pest of citrus, has developed different levels resistance to various acaricides in the field. Abamectin is one most important insecticides/acaricides worldwide, targetting wide number insect and mite pests. evolution abamectin P. threatening sustainable use for control. RESULTS resistant strain (NN‐Aba), derived from field NN by consistent selection with abamectin, showed 4279‐fold compared relatively susceptible (SS) . Cross‐resistance NN‐Aba was observed between emamectin benzoate, pyridaben, fenpropathrin cyflumetofen. Inheritance analyses indicated that autosomal, incompletely recessive polygenic. synergy experiment toxicity synergized piperonyl butoxide (PBO), diethyl maleate (DEM) tributyl phosphorotrithiotate (TPP) strain, ratios were 2.72‐, 2.48‐ 2.13‐fold, respectively. glutathione‐ S‐ transferases activity significantly increased 2.08‐fold SS strain. CONCLUSION polygenic cross‐resistance modes action. Metabolic detoxification mechanism participated These findings provide useful information management © 2023 Society Chemical Industry.

Language: Английский

Citations

12
Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest DOI Creative Commons
Ernesto Villacis‐Perez, Wenxin Xue, Marilou Vandenhole

et al.

Evolutionary Applications, Journal Year: 2023, Volume and Issue: 16(4), P. 863 - 879

Published: March 25, 2023

Abstract Pesticide resistance relies on a myriad of mechanisms, ranging from single mutations to complex and polygenic architecture, it involves mechanisms such as target‐site insensitivity, metabolic detoxification, or combination these, with either additive synergistic effects. Several against abamectin, macrocyclic lactone widely used in crop protection, have been reported the cosmopolitan pest Tetranychus urticae . However, has shown that mechanism cannot account for high levels abamectin found across different mite populations. Here, we experimental evolution combined bulked segregant analyses map quantitative trait loci (QTL) associated two genetically unrelated populations T. In these independent QTL mapping experiments, three four QTLs were identified, which shared between experiments. Shared contained genes encoding subunits glutamate‐gated chloride channel (GluCl) harboured previously mutations, including G314D GluCl1 G326E GluCl3 , but also novel candidate loci, DNA helicases chemosensory receptors. Surprisingly, fourth QTL, present only one experiments thus unique resistant parental line, revealed non‐functional variant GluCl2 suggesting gene knock‐out mechanism. Our study uncovers basis resistance, highlights intraspecific diversity genetic underlying pest.

Language: Английский

Citations

11
Comparative Proteomics of Resistant and Susceptible Strains of Frankliniella occidentalis to Abamectin DOI Creative Commons
Zahra Gholami, Foad Fatehi,

Fatemeh Habibpour Mehraban

et al.

Electrophoresis, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

ABSTRACT Western flower thrips, Frankliniella occidentalis ( Thysanoptera : Thripidae) is an invasive agricultural pest with developed resistance to abamectin in some strains due frequent treatment the pesticide. In this study, we examined differentially expressed proteins (DEPs) between abamectin‐resistant (Aba R ; under selective pressure) and susceptible S without of F. . Proteins were isolated from second instar larvae both separated via two‐dimensional polyacrylamide gel electrophoresis. Nano‐flow liquid chromatography–tandem mass spectrometry identified selected protein spot features. From 70 DEPs, 43 features identified: A total 23 showed increase abundance, 20 down‐regulated response pressure. The enzymatic structural classified into functional groups macromolecular metabolisms, signaling cellular processes, immune system, genetic information processing, exoskeleton‐related proteins. up‐regulation may contribute forming a thicker cuticle, potentially hindering penetration, which interesting finding that needs further investigation. Two novel proteins, triacylglycerol lipase cuticle CPF 2, only Aba This work provides insights mechanisms , will provide important for developing insecticide management approaches pest.

Language: Английский

Citations

0
Two carboxyl/choline esterase (CCE) genes, TuCCE11 and TuCCE34, are related to abamectin resistance in Tetranychus urticae Koch DOI
Yan Zhang, Ke Wang, Dandan Xu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143287 - 143287

Published: April 17, 2025

Language: Английский

Citations

0
A glutamate-gated chloride channel as the mite-specific target-site of dicofol and other diphenylcarbinol acaricides DOI Creative Commons
Marilou Vandenhole, Catherine Mermans, Berdien De Beer

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Nov. 13, 2023

Abstract Dicofol has been widely used to control phytophagous mites. Although dicofol is chemically related DDT, its mode of action remained elusive. Here, we mapped resistance in the spider mite Tetranychus urticae two genomic regions. Each region harbored a glutamate-gated chloride channel (GluCl) gene that contained mutation—G314D or G326E—known confer against unrelated acaricide abamectin. Using electrophysiology assays showed and other diphenylcarbinol acaricides—bromopropylate chlorobenzilate—induce persistent currents Xenopus oocytes expressing wild-type T. GluCl3 receptors potentiate glutamate responses. In contrast, G326E substitution abolished agonistic activity all three compounds. Assays with Drosophila GluClα revealed this receptor was unresponsive dicofol. Homology modeling combined ligand-docking confirmed specificity assays. Altogether, work elucidates diphenylcarbinols as mite-specific agonists GluCl.

Language: Английский

Citations

4
Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch DOI Creative Commons

Ming‐Mei Wu,

Rui Dong, Yan Zhang

et al.

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Pesticide resistance greatly limits control efficacy after the long-term application of pesticides. The two-spotted spider mite, Tetranychus urticae Koch, is a notorious agricultural pest worldwide that resistant to various pesticides, including abamectin. While some studies abamectin have investigated target related glutamate-gated chloride channels (GluCls), on metabolic mechanisms are still limited. In this study, we identified an ABCC subfamily gene, TuABCC4, was overexpressed in populations T. urticae, based analysis previously obtained transcriptomic and RNA-seq data. No consistent nonsynonymous mutations TuABCC4 gene were found between susceptible populations, although expression significantly increased all studied. Synergistic experiments with inhibitor verapamil confirmed key role resistance. addition, increase shown by RNA interference genetic association be closely conclusion, overexpression involved urticae. These results can help us better understand molecular basis

Language: Английский

Citations

1
Acaricide resistance mechanisms and host plant responses in the tomato specialist Aculops lycopersici DOI
Lore Vervaet, Jason Charamis, Marilou Vandenhole

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract BACKGROUND The mite Aculops lycopersici is a major tomato pest with extremely reduced gene families involved in chemoreception and detoxification. How this limited detoxification toolbox affects the evolution of resistance to acaricides russet mite(s) (TRM) remains enigmatic. Moreover, although specialist, TRM has been observed on other Solanaceae Convolvulaceae plant species, raising questions about transcriptional plasticity underlying host exchange. RESULTS We identified field strain strongly decreased susceptibility both abamectin spiromesifen. detected target‐site caused by mutations at conserved positions two glutamate‐gated chloride channels (GluCl), as well four overexpressed genes. then examined responses after shift from (potato black nightshade) (sweet potato hedge bindweed) more challenging plants. Transcriptional varied significantly between families, key differentially expressed genes (DEGs) related proteolytic, metabolic processes. Last, we also DEGs encoding for secreted proteins potentially TRM–host interactions. CONCLUSIONS Despite toolbox, A. might quickly evolve resistance, probably facilitated strong selection pressure genetic variation associated enormous population size settings. Responses changes include digestion detoxification, while most responsive are unknown function remain be investigated. © 2024 Society Chemical Industry.

Language: Английский

Citations

1
Independent genetic mapping experiments identify diverse molecular determinants of host adaptation in a generalist herbivore DOI
Ernesto Villacis‐Perez, Femke De Graeve, Berdien De Beer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 8, 2024

Abstract Interactions between plants and herbivores promote evolutionary change. Studying the evolution of herbivore mechanisms aimed to cope with diUerent host plant species is a critical intersection biology sustainable pest management. Generalist are particular interest, as hybridization genetically distinct populations can increase standing genetic variation therefore adaptive potential species. Tetranychus urticae generalist arthropod known for its potential, evidenced in immense range ability develop metabolic resistance xenobiotics. However, molecular underpinnings associated adaptation consequences this many other pests remain elusive. Here, we use two independent, empirical approaches identify map basis performance T. . In first approach, subject diverse mite population tomato selection genomic regions linked phenotypic increased reproductive performance. second responsible on by comparing genomes pooled individuals from F2 backcross high low Both revealed specific shared key candidate genes were identified. Our findings highlight power spider complex herbivore.

Language: Английский

Citations

0