Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237

Published: March 5, 2024

Language: Английский

---

Humanized brain organoids-on-chip integrated with sensors for screening neuronal activity and neurotoxicity

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(1)

Published: Jan. 1, 2024

Language: Английский

---

Organoids‐On‐a‐Chip for Personalized Precision Medicine

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 13, 2024

Abstract The development of personalized precision medicine has become a pivotal focus in modern healthcare. Organoids‐on‐a‐Chip (OoCs), groundbreaking fusion organoid culture and microfluidic chip technology, emerged as promising approach to advancing patient‐specific treatment strategies. In this review, the diverse applications OoCs are explored, particularly their role medicine, potential cutting‐edge technology is highlighted. By utilizing patient‐derived organoids, offer pathway optimize treatments, create precise disease models, investigate mechanisms, conduct drug screenings, individualize therapeutic emphasis on significance technological revolutionizing healthcare improving patient outcomes. Furthermore, transformative future prospects, ongoing advancements field, with genomic multi‐omics integration, ethical frameworks discussed. convergence these innovations can empower patients, redefine approaches, shape

Language: Английский

---

Development of brain organoid technology derived from iPSC for the neurodegenerative disease modelling: a glance through

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Aug. 3, 2023

Neurodegenerative diseases are adult-onset neurological conditions that notoriously difficult to model for drug discovery and development because most models unable accurately recapitulate pathology in disease-relevant cells, making it extremely explore the potential mechanisms underlying neurodegenerative diseases. Therefore, alternative of human or animal cells have been developed bridge gap allow impact new therapeutic strategies be anticipated more by trying mimic neuronal glial cell interactions many mechanisms. In tandem with emergence human-induced pluripotent stem which were first generated 2007, accessibility (hiPSC) derived from patients can differentiated into neurons, providing an unrivaled platform

Language: Английский

---

Technology using simulated microgravity

Regenerative Therapy, Journal Year: 2023, Volume and Issue: 24, P. 318 - 323

Published: Aug. 22, 2023

The human body experiences constant stimulation from Earth's gravity, and the absence of gravity leads to various impacts at cellular tissue levels. Simulated microgravity (s-μg) has been employed on Earth investigate these effects, circumventing challenges conducting experiments in space providing an opportunity understand influence living organisms. Research focusing stem cells utilizing s-μg enhanced our understanding how affects cell morphology, migration, proliferation, differentiation. Studies have used systems such as rotating wall vessels, random positioning machines, clinostats. By uncovering mechanisms underlying observed changes studies, there is potential identify therapeutic targets that regulate function explore a range applications, including cell-based regenerative medicine. This review will focus features each device designed simulate Earth, well performed with those devices.

Language: Английский

---

Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics

Biotechnology and Bioengineering, Journal Year: 2023, Volume and Issue: 121(2), P. 489 - 506

Published: Nov. 28, 2023

Brain organoids are self-organized, three-dimensional (3D) aggregates derived from pluripotent stem cells that have cell types and cellular architectures resembling those of the developing human brain. The current understanding brain developmental processes neurological disorders has advanced significantly with introduction this in vitro model. serve as a translational link between two-dimensional (2D) cultures vivo models which imitate neural tube formation at early late stages differentiation neuroepithelium whole-brain regionalization. In addition, generation region-specific made it possible to investigate pathogenic etiological aspects acquired inherited disease along drug discovery toxicity testing. review article, we first summarize an overview existing methods platforms used for generating their limitations then discuss recent advancement organoid technology. how been model neurodevelopmental neurodegenerative diseases, including autism spectrum disorder (ASD), Rett syndrome, Zika virus-related microcephaly, Alzheimer's (AD), Parkinson's (PD), Huntington's (HD).

Language: Английский

---

Advances in the knowledge and therapeutics of schizophrenia, major depression disorder, and bipolar disorder from human brain organoid research

Frontiers in Psychiatry, Journal Year: 2023, Volume and Issue: 14

Published: July 12, 2023

Tridimensional cultures of human induced pluripotent cells (iPSCs) experimentally directed to neural differentiation, termed “brain organoids” are now employed as an in vitro assay that recapitulates early developmental stages nervous tissue differentiation. Technical progress culture methodology enabled the generation regionally specialized organoids with structural and neurochemical characters distinct encephalic regions. The technical process organoid elaboration is undergoing progressively implementation, but current robustness has attracted attention psychiatric research substitute/complement animal experimentation for analyzing pathophysiology disorders. Numerous morphological, structural, molecular functional insights disorders have been uncovered by comparing brain made iPSCs obtained from control healthy subjects patients. Brain were also response conventional treatments, search new drugs, anticipate therapeutic individual patients a personalized manner. In this review, we gather data studying cerebral three most frequent serious disorders: schizophrenia, major depression disorder, bipolar disorder. Among these studies, emphasize: (i) origin pathologies takes place embryonic development; (ii) existence shared pathogenic aspects among disorders; (iii) occurrence differences between bearing same (iv) alterations can be activated or aggravated environmental signals genetic risk

Language: Английский

---

A Low‐Cost, User‐Friendly Rheo‐Optical Compression Assay to Measure Mechanical Properties of Cell Spheroids in Standard Cell Culture Plates

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: 9(4)

Published: Jan. 4, 2024

Abstract The mechanical characterization of cell spheroids, one the most widely used 3D biology models in vitro, is a hotspot current research on role played by response cells and tissues. techniques proposed so far literature, while providing important scientific insights, require specialized equipment technical skills that are not usually available facilities. Here, an innovative rheo‐optical compression assay presented: it based microscopy glass coverslips as load applied to spheroids standard culture plates image acquisition with optical microscope or even smartphone equipped adequate magnification lenses. Mechanical properties can be simply obtained correlating deformation measured analysis. low‐cost, user‐friendly features technique boost mechanobiology making easily affordable any biomedical lab

Language: Английский

---

Live Organoid Cyclic Imaging

Advanced Science, Journal Year: 2024, Volume and Issue: 11(14)

Published: Feb. 7, 2024

Abstract Organoids are becoming increasingly relevant in biology and medicine for their physiological complexity accuracy modeling human disease. To fully assess biological profile while preserving spatial information, spatiotemporal imaging tools warranted. While previously developed techniques, such as four‐dimensional (4D) live light‐sheet have yielded important clinical insights, these technologies lack the combination of cyclic multiplexed analysis. address challenges, bioorthogonal click chemistry is applied to display first demonstration fixed patient‐derived glioblastoma tumor organoids. This technology exploits quench fluorescent signals from surface intracellular labeled cells across multiple cycles, allowing more accurate efficient molecular profiling complex phenotypes. Herein, versatility this demonstrated screening markers organoids conserving viability. It anticipated that findings applications work can be broadly translated into investigating developments other organoid systems.

Language: Английский

---

Comprehensive analysis of resilience of human airway epithelial barrier against short‐term PM2.5 inorganic dust exposure using in vitro microfluidic chip and ex vivo human airway models

Allergy, Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract Background and Objective The updated World Health Organization (WHO) air quality guideline recommends an annual mean concentration of fine particulate matter (PM2.5) not exceeding 5 or 15 μg/m 3 in the short‐term (24 h) for no more than 3–4 days annually. However, 90% global population is currently exposed to daily concentrations surpassing these limits, especially during extreme weather conditions due transboundary dust transport influenced by climate change. Herein, effect respirable <PM2.5 inorganic silica particle exposures on epithelial barrier integrity was simultaneously evaluated within biomimetic microfluidic platform‐based airway (AEB)‐on‐a‐chip human bronchoscopic ex vivo tissue models, comparatively. Methods Silica particles at average size 1 μm, referred as <PM2.5, dose‐dependently tested MTT LDH analyses. elicited dose 800 μg/mL applied cells (Calu‐3) seeded membrane air–liquid interface AEB‐on‐a‐chip platform, which operated under static dynamic bronchoscopy bronchial slices 72 h. For both healthy groups were comparatively investigated. Computational fluid dynamics simulations performed assess shear stress profiles different flow conditions. Qualitative quantitative analyses carried out evaluate resilience via cell survivability, morphology, integrity, permeability, inflammation. Results In exposure PM2.5 disrupted AEB increasing decreasing adhesion‐barrier markers such ZO‐1 , Vinculin ACE2 CD31 impaired viability increased expression levels proinflammatory markers; IFNs IL‐6 IL‐1s TNF‐α CD68 CD80 Inos mostly Besides, decreased viability, β‐catenin E‐cadherin also response with elevated IL‐1 α, IFN‐Ɣ markers, observed after tissue. Conclusion duration that can be natural events aligns our model (0–800 h). At this level exposure, demonstrated platform emulating forces body biopsy slices. Lung‐on‐a‐chip models will serve reliable context.

Language: Английский

---