Experimental Parasitology, Journal Year: 2024, Volume and Issue: 260, P. 108746 - 108746
Published: March 19, 2024
Language: Английский
BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 21, 2025
Language: Английский
Citations
3
Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30539 - e30539
Published: May 1, 2024
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 29, 2024
Abstract Ticks, hematophagous acari, pose a significant threat by transmitting various pathogens to their vertebrate hosts during feeding. Despite advances in tick genomics, high-quality genomes were lacking until recently, particularly the genus Ixodes , which includes main vectors of Lyme disease. Here, we present complete genome sequences four species, derived from single female individual, with particular focus on European species ricinus achieving chromosome-level assembly. Additionally, draft assemblies generated for three other I. persulcatus, pacificus and hexagonus . The quality extensive annotation several important gene families have allowed us study evolution repertoires at level group. We determined that undergone major amplifications ticks, while an expression atlas obtained reveals striking patterns specialization both between within families. Notably, family are associated proliferation single-exon genes. integration our data existing establishes solid framework evolution, improving understanding biology. In addition, work lays foundations applied research innovative control targeting these organisms.
Language: Английский
Citations
3
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: Aug. 13, 2024
Ticks are obligate hematophagous arthropods that transmit a wide range of pathogens to humans as well wild and domestic animals. They also harbor non-pathogenic microbiota, although our previous study has shown the diverse bacterial microbiome in midgut Ixodes ricinus is quantitatively poor lacks core. In artificial infections by capillary feeding ticks with two model bacteria (Gram-positive Micrococcus luteus Gram-negative Pantoea sp.), rapid clearance these microbes from was observed, indicating presence active immune mechanisms this organ. current study, RNA-seq analysis performed on I. females inoculated either M. or sp. sterile water control. While no immune-related transcripts were upregulated microbial inoculation compared control, itself triggered dramatic transcriptional changes tick midgut. Manual curation transcriptome unfed females, complemented proteomic analysis, revealed several constitutively expressed putative antimicrobial peptides (AMPs) independent stimulation referred here ‘guard’ AMPs. These included types midgut-specific defensins, different domesticated amidase effector 2 (Dae2), microplusin/ricinusin-related molecules, lysozymes, gamma interferon-inducible lysosomal thiol reductases (GILTs). The vitro activity assays synthetic mature defensin 1 8, confirmed their specificity against Gram-positive showing exceptional potency inhibit growth at nanomolar concentrations. defensins likely part multicomponent system responsible for Further studies needed evaluate role other identified AMPs controlling microorganisms entering
Language: Английский
Citations
2
Insects, Journal Year: 2024, Volume and Issue: 15(6), P. 453 - 453
Published: June 14, 2024
iGABAR, a member of the Cys-loop ligand-gated ion channel superfamily, is significant target insecticide ivermectin (IVM). GRD potential subunit insect iGABAR. However, little information about in Ae. aegypti has been reported. In this study, we involved cloning and characterizing iGABAR (Ae-GRD). Sequence analysis indicated that Ae-GRD, as part cysteine-loop family, similar to other GRD. RNA interference (RNAi) was employed explore IVM resistance aegypti, resulting reduction Ae-GRD expression (p < 0.05), mortality adults with knockdown significantly decreased after exposure ivermectin. Bioinformatics prediction identified miR-71-5p regulator Ae-GRD. vitro, dual-luciferase reporter assays confirmed regulated by miR-71-5p. Microinjection mimics upregulated downregulated gene expression, reducing 34.52% following treatment. Conversely, microinjection inhibitor but did not affect susceptibility despite increased 0.05). conclusion, one iGABA receptor subunits, It may influence modulating GABA signaling pathway. The inhibition consequently lowered rate mosquitoes. This finding provides empirical evidence relationship between its miRNA resistance, offering novel perspectives for mosquito control strategies.
Language: Английский
Citations
2
Viruses, Journal Year: 2023, Volume and Issue: 15(11), P. 2154 - 2154
Published: Oct. 25, 2023
Half a century has passed since the discovery of Pseudomonas phage phi6, first enveloped dsRNA bacteriophage to be isolated. It remained sole known for quarter and only recognised member Cystoviridae family until year 2018. After initial additional phages have been isolated from globally distant locations identified in metatranscriptomic datasets, suggesting that this virus type is more ubiquitous nature than previously acknowledged. Most infect strains utilise either pilus or lipopolysaccharide components host as primary receptor. In addition receptor-mediated strictly lytic lifestyle, an alternative persistent infection strategy described some phages. To date, complete genome sequences fourteen isolates are available. Despite high sequence diversity, similar sets genes can typically found genomes phages, shared evolutionary trajectories. This review provides brief overview members related isolates, outlines current classification discusses their relationships with eukaryotic RNA viruses.
Language: Английский
Citations
5
Trends in Parasitology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
1
Experimental Parasitology, Journal Year: 2024, Volume and Issue: 260, P. 108746 - 108746
Published: March 19, 2024
Language: Английский
Citations
0