Unveiling Mir-101a as a Molecular Mediator in Triclosan-Induced Lipid Metabolism Disruption: Implications for Obesity and Metabolic Dysfunction DOI
Jin Yan,

Jinyun Li,

Yang Wang

et al.

Published: Jan. 1, 2023

As a potential environmental obesogen, triclosan (TCS) carries inherent risks of inducing obesity and metabolic disorders. However, the underlying molecular mechanisms behind lipid metabolism disorder induced by TCS have remained elusive. Through fusion transcriptomics microRNA target prediction, our investigation supposes that miR-101a as responsive miRNA to exposure in zebrafish, playing central role disturbing homeostasis. evidence triggers reduction expression, accompanied elevation genes linked precision-controlled interventions involving expression modulation, we discovered inhibition heightened its implicated homeostasis, subsequently triggering excessive fat accumulation. Meanwhile, overexpression acts protective mechanism, counteracting larvae. Notably, combination short-chain fatty acids (SCFAs) emerged remedy alleviate TCS-induced accumulation partially decline TCS. These revelations provide insight into prospective framework TCS-triggered disorders, thereby paving way for pre-emptive strategies combating ramifications pollution.

Language: Английский

From fatty liver to fibrosis: the impact of miRNAs on NAFLD and NASH DOI
Reda Mansour,

Sherif S Abdel Mageed,

Ahmed I. Abulsoud

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 31, 2025

Language: Английский

Citations

1
A crosstalk between epigenetic modulations and non-alcoholic fatty liver disease progression DOI

Meysam Ramezani,

Mahsa Zobeiry,

Shahrokh Abdolahi

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 251, P. 154809 - 154809

Published: Sept. 16, 2023

Language: Английский

Citations

13
MicroRNAs in the Diagnosis of Digestive Diseases: A Comprehensive Review DOI Open Access

Mirela Livia Popa,

Cristian Ichim,

Paula Anderco

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(6), P. 2054 - 2054

Published: March 18, 2025

MicroRNAs (miRNAs) have emerged as crucial regulators in digestive pathologies, including inflammatory bowel disease (miR-31, miR-155, and miR-21), colorectal cancer (miR-21, miR-598, miR-494), non-alcoholic fatty liver miR-192, miR-122). Their capacity to modulate gene expression at the post-transcriptional level makes them highly promising candidates for biomarkers therapeutic interventions. However, despite considerable progress, their clinical application remains challenging. Research has shown that miRNA is dynamic, varying across patients, stages, different intestinal regions. dual function both oncogenes tumor suppressors further complicates use, targeting miRNAs may yield unpredictable effects. Additionally, while miRNA-based therapies hold great potential, significant hurdles persist, off-target effects, immune activation, inefficiencies delivery methods. The intricate interplay between gut microbiota adds another layer of complexity, influencing mechanisms treatment responses. This review examined role emphasizing diagnostic potential. While they offer new avenues management, unresolved challenges underscore need research refine application.

Language: Английский

Citations

0
Deciphering the molecular mediators of triclosan-induced lipid accumulation: Intervention via short-chain fatty acids and miR-101a DOI
Jin Yan,

Jinyun Li,

Yang Wang

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 343, P. 123153 - 123153

Published: Dec. 14, 2023

Language: Английский

Citations

8
The Molecular Mechanism of circRNA-11228/miR-103/INSIG1 Pathway Regulating Milk Fat Synthesis in Bovine Mammary Epithelial Cells DOI Creative Commons
Xiaofen Li, Yanni Wu, Yuhao Wang

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(4), P. 538 - 538

Published: March 28, 2024

Milk, known for its high content of short- and medium-chain fatty acids unsaturated acids, has attracted substantial attention due to nutritional health value. The regulation acid metabolism by non-coding RNAs become a subject growing attention, particularly in relation production at the transcriptional/epigenetic post-transcriptional levels. This study established circRNA-11228/miR-103/INSIG1 (insulin-inducible gene) regulatory network using methods such as qRT-PCR, dual luciferase reporting, Western blot, with INSIG1 serving starting point. experimental validation circRNA-11228’s impact on cholesterol levels, lipid droplet secretion, was conducted various assays, including triglycerides, cholesterol, oil red O, andEdU(5-ethynyl-2’-deoxyuridine) bovine mammary epithelial cells (BMECs). Furthermore, transfection mimics inhibitors synthesized from miR-103 into BMECs confirmed that can promote synthesis secretion. Conversely, gene found inhibit “remediation” experiment validated ability alleviate cellular effect circRNA-11228. Taken together, our findings indicate binding circRNA-11228 inhibits expression target INSIG, thereby regulating milk fat BMECs. offers novel insights producing high-quality new ways improve dietary composition residents.

Language: Английский

Citations

1
Lipid Regulatory Element Interact with CD44 on Mitochondrial Bioenergetics in Bovine Adipocyte Differentiation and Lipometabolism DOI
Guanghui Li, Xibi Fang,

Yinuo Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(31), P. 17481 - 17498

Published: July 29, 2024

The CD44 gene is a critical factor in animal physiological processes and has been shown to affect insulin resistance fat accumulation mammals. Nevertheless, little research conducted on its precise functions lipid metabolism adipogenic differentiation beef cattle. This study analyzed the expression of miR-199a-3p during bovine preadipocyte differentiation. luciferase reporter assay demonstrated that was direct target miR-199a-3p. Increased droplets triglyceride levels, altered fatty acid metabolism, accelerated were all caused by upregulation or reduction expression. knockdown upregulated adipocyte-specific genes (LPL FABP4) levels metabolites (SOPC, l-arginine, heptadecanoic acid). Multiomics highlights enriched pathways involved energy (MAPK, cAMP, calcium signaling) shifts mitochondrial respiration glycolysis, indicating plays regulatory role metabolism. findings show intracellular lipolysis, respiration, deposition, droplet composition are impacted miR-199a-3p, which modulates adipocytes.

Language: Английский

Citations

0
Differential Hepatic Expression of miRNA in Response to Aflatoxin B1 Challenge in Domestic and Wild Turkeys DOI Creative Commons

Kade Jorud,

Kristelle M. Mendoza,

Thomas J. Y. Kono

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(11), P. 453 - 453

Published: Oct. 22, 2024

Aflatoxin B1 (AFB

Language: Английский

Citations

0
Unveiling Mir-101a as a Molecular Mediator in Triclosan-Induced Lipid Metabolism Disruption: Implications for Obesity and Metabolic Dysfunction DOI
Jin Yan,

Jinyun Li,

Yang Wang

et al.

Published: Jan. 1, 2023

As a potential environmental obesogen, triclosan (TCS) carries inherent risks of inducing obesity and metabolic disorders. However, the underlying molecular mechanisms behind lipid metabolism disorder induced by TCS have remained elusive. Through fusion transcriptomics microRNA target prediction, our investigation supposes that miR-101a as responsive miRNA to exposure in zebrafish, playing central role disturbing homeostasis. evidence triggers reduction expression, accompanied elevation genes linked precision-controlled interventions involving expression modulation, we discovered inhibition heightened its implicated homeostasis, subsequently triggering excessive fat accumulation. Meanwhile, overexpression acts protective mechanism, counteracting larvae. Notably, combination short-chain fatty acids (SCFAs) emerged remedy alleviate TCS-induced accumulation partially decline TCS. These revelations provide insight into prospective framework TCS-triggered disorders, thereby paving way for pre-emptive strategies combating ramifications pollution.

Language: Английский

Citations

0