bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 21, 2023
ABSTRACT
Ammonia
availability
has
a
crucial
role
in
agriculture
as
it
ensures
healthy
plant
growth
and
increased
crop
yields.
Since
diazotrophs
are
the
only
organisms
capable
of
reducing
dinitrogen
to
ammonium,
they
have
great
ecological
importance
potential
mitigate
environmental
economic
costs
synthetic
fertilizer
use.
Rhizobia
especially
valuable
being
that
can
engage
nitrogen-fixing
symbiotic
relationships
with
legumes,
demonstrate
diversity
plasticity
genomic
phenotypic
traits.
However,
few
rhizobial
species
improved
genetic
tractability
for
biology
applications.
This
study
established
basic
toolbox
antibiotic
resistance
markers,
multi-host
shuttle
plasmids
streamlined
protocol
biparental
conjugation
Mesorhizobium
Bradyrhizobium
species.
We
identified
two
repABC
origins
replication
from
Sinorhizobium
meliloti
(pSymB)
Rhizobium
etli
(p42d)
were
stable
across
all
three
strains
interest.
Furthermore,
NZP2235
genome
was
sequenced
phylogenetic
analysis
determined
its
reclassification
huakuii
.
These
tools
will
enable
use
plasmid-based
strategies
more
advanced
engineering
projects
ultimately
contribute
towards
development
sustainable
practices
by
means
novel
organelles,
elite
bioinoculants
or
association
non-legumes.
GRAPHICAL
Plant Production Science,
Journal Year:
2024,
Volume and Issue:
27(3), P. 155 - 169
Published: June 29, 2024
Intercropping
is
an
agricultural
technique
where
many
crops
are
grown
together
on
the
same
field,
and
it
becoming
more
widely
acknowledged
for
its
ability
to
improve
productivity
in
food
forage
production.
This
farming
method
allows
strategic
integration
of
a
profitable
crop
with
cover
or
non-profitable
achieve
reciprocal
benefits,
while
also
spreading
advantages
across
different
systems.
designs
differ
attain
greater
yields,
reduce
use
chemicals
fertilizers,
enhance
water-use
efficiency,
especially
demanding
circumstances
arid
semi-arid
countries.
study
examines
new
mechanisms
via
which
intercropping
enhances
sustainable
agriculture,
including
recent
progress
ecological,
genetic,
microbial
interactions
that
plant
growth
resilience.
The
adaptability
Mediterranean
region,
varied
methods
assessed,
particular
focus
possibilities
implementing
provides
thorough
analysis
existing
research,
emphasizing
contribution
agriculture.
BMC Plant Biology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 16, 2025
Drought
has
a
significant
impact
on
ecosystem
functions,
especially
the
biogeochemical
cycling
of
phosphorus
(P),
which
is
crucial
nutrient
for
plant
growth
and
productivity.
Despite
its
importance,
effects
different
drought
scenarios
soil
P
availability
remain
poorly
understood
in
previous
studies.
This
study
simulated
conditions
tropical
soils
using
maize
as
test
crop
under
varying
field
capacity
(FC)
levels
(100%,
80%,
60%,
40%,
20%)
over
60-day
pot
experiment.
uptake
biomass
decreased
significantly
lower
FC
level.
was
highest
at
FC100
(5
g
kg-¹)
lowest
FC20
(3.5
kg-¹).
Similarly,
greatest
(70
plant-¹)
declined
to
35
plant-¹
FC20,
underscoring
adverse
growth.
The
results
showed
substantial
increase
calcium-associated
(HClD-Pi),
reaching
45%
FC20.
Conversely,
labile
inorganic
fractions
(NaHCO₃-Pi
NaOH-Pi)
significantly,
from
14.73
6.2
mg
kg-¹
29.4
17.7
kg-¹,
respectively,
compared
FC100.
Organic
(NaHCO₃-Po,
NaOH-Po)
increased
by
6
2.4
times,
treatments,
while
HClc-Po
also
elevated
drier
conditions.
These
transformations
were
attributed
changes
pH
calcium
content,
favoring
stabilization
HClD-Pi.
disrupted
replenishment
solution,
reducing
bioavailability,
though
phosphatase
activity
enhanced
organic
release.
Pearson's
correlation
analysis
revealed
positive
associations
between
moderately
(NaHCO₃-Pi,
NaOH-Pi,
HClD-Pi)
elements
(Ca,
Al,
Fe).
RDA
highlighted
link
reduced
P,
strongly
associated
with
fractions.
findings
demonstrate
drought's
cycling,
dynamics.
Frontiers in Sustainable Food Systems,
Journal Year:
2025,
Volume and Issue:
9
Published: Feb. 21, 2025
Adopting
sustainable
agricultural
practices
that
enhance
productivity
while
preserving
ecosystem
services
is
essential
to
ensure
food
security
for
a
growing
global
population
and
address
environmental
challenges.
This
review
examines
the
impact
of
legume
intercropping
on
nitrogen
(N)
fixation,
soil
physio-chemical
properties,
water
retention,
pest
disease
control,
crop
yield
across
diverse
agro-climatic
zones
cropping
systems.
The
findings
consistently
demonstrate
integrating
legumes
into
system
improves
health
by
reducing
bulk
density,
breaking
up
hardpan
layers,
erosion,
increasing
organic
matter,
fixing
atmospheric
(~125
kg
N/ha/season)
need
inorganic
N
fertilizers.
It
boosts
yields
30–35%
(in
terms
main
equivalent
yield)
land
per
unit
area
time,
mitigates
total
loss,
promotes
biodiversity.
also
use
efficiency
20–25%
enhances
nutrient
25–30%.
Additionally,
reduces
losses
from
pests
diseases
compared
sole
practice
bolsters
resilience
through
ecological
processes
like
bio-littering,
bio-ploughing,
bio-irrigation,
bio-pumping
(the
“4Bs”),
which
are
valuable
adapting
climate
variability.
However,
research
gaps
remain,
particularly
in
optimal
selection
species
specific
regions,
suitable
agronomic
each
system,
addressing
socio-economic
barriers
widespread
adoption.
Agriculture,
Journal Year:
2024,
Volume and Issue:
14(12), P. 2228 - 2228
Published: Dec. 5, 2024
Drought
is
an
increasingly
critical
global
challenge,
significantly
impacting
agricultural
productivity,
food
security,
and
ecosystem
stability.
As
climate
change
intensifies
the
frequency
severity
of
drought
events,
innovative
strategies
are
essential
to
enhance
plant
resilience
sustain
systems.
This
review
explores
vital
role
beneficial
microbes
in
conferring
tolerance,
focusing
on
Plant
Growth-Promoting
Rhizobacteria
(PGPR),
mycorrhizal
fungi,
endophytes,
actinomycetes,
cyanobacteria.
These
microorganisms
mitigate
stress
through
diverse
mechanisms,
including
osmotic
adjustment,
enhancement
root
architecture,
modulation
phytohormones,
induction
antioxidant
defenses,
regulation
stress-responsive
gene
expression.
Ecological
innovations
leveraging
these
have
demonstrated
significant
potential
bolstering
resilience.
Strategies
such
as
soil
microbiome
engineering,
bioaugmentation,
integration
microbial
synergies
within
pest
management
frameworks
sustainability.
Additionally,
advancements
practices,
seed
coating,
amendments,
development
consortia,
precision
agriculture
technologies,
validated
effectiveness
scalability
interventions
farming
Despite
promising
advancements,
several
challenges
hinder
widespread
adoption
solutions.
Environmental
variability
can
affect
performance,
necessitating
robust
adaptable
strains.
Scale-up
commercialization
hurdles,
economic
constraints,
regulatory
safety
considerations
also
pose
barriers.
Furthermore,
complex
interactions
between
microbes,
plants,
their
environments
require
a
deeper
understanding
optimize
benefits
consistently.
Future
research
should
focus
integrating
cutting-edge
technologies
genomics,
synthetic
biology,
refine
interventions.
Collaborative
efforts
among
academia,
industry,
government
bridge
gap
practical
implementation.
By
addressing
harnessing
innovations,
it
possible
develop
resilient
sustainable
systems
capable
thriving
water-scarce
world.
Plant Stress,
Journal Year:
2024,
Volume and Issue:
12, P. 100462 - 100462
Published: April 17, 2024
Maize,
a
vital
crop
for
human
nutrition,
livestock,
and
industrial
development,
faces
increasingly
severe
climatic
conditions
that
hinder
its
production
capacity
along
with
other
strategic
crops.
Novel
treatments
based
on
microorganisms
have
demonstrated
efficiency
in
enhancing
plant
development
responding
to
stress.
The
use
of
bacteria
isolated
from
seeds
is
novel
approach
biotreatment,
as
recent
studies
point
co-evolution
process
their
presence
seeds.
This
hypothesize
pre-adaptation
host,
which
may
lead
increased
efficiency.
However,
several
aspects
this
remain
understudied.
In
study,
we
aimed
evaluate
the
potential
native
maize
seed
microbiota
comparison
species
mitigate
drought
For
characterized
seedborne
common
productive
variety
Portugal,
it
biotreatment
two
varieties
(sensitive
resistant
drought),
selecting
strain
Pseudomonas
fulva
MB
most
promising
candidate.
Stenotrophomonas
maltophilia
MS-M1
strain,
wild
alfalfa
previously
drought-tolerant
enhancer,
served
non-native
control
strain.
.
According
data,
both
enhanced
vegetative
growth
when
treated
individual
strains,
well
consortium,
an
increase
height
5-7%
full
medium
irrigation,
50-55%
not
irrigated.
trend
was
also
observed
weight,
by
13-23%,
even
under
no
irrigation.
addition,
positively
impacted
these
treatments,
amount
grain
produced
(by
weight).
drought-sensitive
experienced
17%
while
tolerant
25-40%
increase.
Under
irrigation
level,
about
15%
varieties,
100%
140%
each
variety,
respectively,
applied.
results
suggest
P.
slightly
more
efficient
treatment,
outperformed
terms
productivity
varieties.
differences
were
solid
enough
all
parameters
consistently
asses
such
difference.
consortium
treatment
only
showed
better
performance
or
some
traits.
These
findings
support
very
biotreatments,
suggesting
than
strains
remarkable
beneficial
impact
(interspecific),
expanding
possible
kind
bioinoculants.
Further
test
are
required
optimize
seed-isolated
adapted
tailor-made
solutions
agriculture.
