A newly identified Y chromosome gene obp‐Y is required for sperm storage in female Zeugodacus tau (Diptera: Tephritidae) DOI
Peipei Liu,

Ziniu Li,

Imran Afzal

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Abstract In the organisms with XX/XY sex chromosomes, Y chromosome is unique to males and plays an important role in male reproductive development. The study of genes will contribute development pest genetic prevention control technology. this study, we identified 9 Zeugodacus tau (Diptera: Tephritidae), including gene 16222 . Protein structure analysis showed that was highly similar odorant binding protein, thus named obp‐Y Obp‐Y knockout (KO) significantly reduced hatching rate offspring. Sperm detection results KO did not affect sperm number testes or transfer during mating. We further examined storage sperms females, found females mating wild‐type began from spermathecal ducts spermathecae at hour 0 after end (AEM), 0–24 h AEM, count gradually increased. However, no were observed mutant hours 0, 4, 8, 24 48 AEM. summary, revealed necessary for females. Our findings only provide theoretical basis elucidating function chromosome, but also offer a molecular target over Z.

Language: Английский

Testis-specific serine/threonine kinase dTSSK2 regulates sperm motility and male fertility in Drosophila DOI Creative Commons
Peng Ju,

Angyang Sun,

Jie Zheng

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: May 7, 2025

Language: Английский

Citations

0
Y chromosome genes interplay with interval timing in regulating mating duration of male Drosophila melanogaster DOI
Huang Yongwen, Alex C. Kwan, Woo Jae Kim

et al.

Gene Reports, Journal Year: 2024, Volume and Issue: 36, P. 101999 - 101999

Published: Aug. 2, 2024

Language: Английский

Citations

1
Paralog-dependent Specialization of Paf1C subunit, Ctr9, for Sex Chromosome Gene Regulation and Male Germline Differentiation in Drosophila DOI
Toshie Kai,

Jinglan Zheng,

Taichiro Iki

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 26, 2024

Abstract Testis-specific gene regulatory mechanisms govern the differentiation of germ cells into mature sperms. However, molecular underpinnings are not fully elucidated. Here, we show subunits forming Polymerase Associated Factor 1 Complex (Paf1C), a transcription regulator conserved across eukaryotes, have their individual paralogs predominantly expressed in Drosophila testes. One these, namely, Ctr9 paralog enriched testes (Ctr9t) was found to play critical and nonredundant role post-meiotic spermatid male fertility D. melanogaster . A proximity proteome analysis provides evidence that Ctr9t forms complexes with other members. We endogenous is at spermatocyte stages, accumulating distinct compartment within nucleolus. There, co-localizes Spermatocyte arrest (Sa), testis-specific TATA-binding protein (TBP)-associated factor 8 (TAF8). further demonstrate ctr9t function crucial for maintaining Sa nucleolus, but vice versa Transcriptome profiling reveals acts as an activator set genes on Y chromosome, it also global repressor X chromosome genes. Collectively, our results shed light nucleolus-associated, paralog-dependent regulation expression from sex chromosomes, which ensures terminal cells. Author summary wide repertoire takes coordinated action during sperm formation. spermatogenesis controlled by heretofore unelucidated mechanisms. In this study, expresses called Paf1C. generated loss allele one members named Ctr9t, fertility. Therefore, has established spermatogenesis. Using newly antibodies, stages and, intriguingly, interacts key essential meiotic processes. By performing whole testis transcriptome analyses, reveal chromosomes. Importantly, activation located relies function, can explain defects observed mutant Altogether, propose player nucleolus where controls functional

Language: Английский

Citations

0
The assembly of Y chromosome reveals amplification of genes regulating male fertility inBactrocera dorsalis DOI Creative Commons
Shuangxiong Wu, Jiahong Wu,

Quan Lei

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 3, 2024

Abstract BACKGROUND The oriental fruit fly Bactrocera dorsalis is an invasive pest causing considerable ecological and economic damage worldwide. Y chromosome important target for the sterile insect technique (SIT) but its sequence structure has been poorly explored. RESULTS We assembled genome of B. at level with a total size 562.6 Mb. assembly includes ∼7.6 Mb sequence, being first reported in Tephritidae. highly repetitive, contains 61 genes, including 9 multi-copy genes. surprisingly found that M factor ( MoY ) Tephritidae multiple copies, verified by our droplet digital PCR (ddPCR) analysis. Besides, we identified 32 copies gyf-like on gyfY were expressed testis. RNAi targeting resulted depletion live sperms, suggesting amplification essential male fertility, which facilitated understanding high fecundity this insect. CONCLUSION firstly . Our results will also provide genes CRISPR/Cas9 based SIT, leading to development novel control strategies against tephritid flies.

Language: Английский

Citations

0
Diverse somatic Transformer and sex chromosome karyotype pathways regulate gene expression in Drosophila gonad development DOI Creative Commons
Sharvani Mahadevaraju, Soumitra Pal, Pradeep Kumar Bhaskar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 12, 2024

The somatic sex determination gene

Language: Английский

Citations

0
The Assembly of the Y Chromosome Reveals Amplification of Genes Regulating Male Fertility in Bactrocera Dorsalis DOI
Shuangxiong Wu, Jiahong Wu,

Quan Lei

et al.

Published: Sept. 25, 2024

BACKGROUND: The oriental fruit fly _Bactrocera dorsalis_ is an invasive pest causing considerable ecological and economic damage worldwide. Y chromosome important target for the sterile insect technique (SIT) but its sequence structure has been poorly explored. RESULTS: We assembled genome of _B. at level with a total size 562.6 Mb. assembly includes ∼7.6 Mb sequence, being first reported in Tephritidae. highly repetitive, contains 61 genes, including 9 multi-copy genes. surprisingly found that M factor (_MoY_) Tephritidae multiple copies, verified by our droplet digital PCR (ddPCR) analysis. Besides, we identified 32 copies _gyf-like on chromosome_ (_gyfY_) were expressed testis. RNAi targeting _gyfY_ resulted depletion live sperms, suggesting amplification essential male fertility, which facilitated understanding high fecundity this insect. CONCLUSION: firstly dorsalis_. Our results will also provide genes CRISPR/Cas9 based SIT, leading to development novel control strategies against tephritid flies.

Language: Английский

Citations

0
A newly identified Y chromosome gene obp‐Y is required for sperm storage in female Zeugodacus tau (Diptera: Tephritidae) DOI
Peipei Liu,

Ziniu Li,

Imran Afzal

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Abstract In the organisms with XX/XY sex chromosomes, Y chromosome is unique to males and plays an important role in male reproductive development. The study of genes will contribute development pest genetic prevention control technology. this study, we identified 9 Zeugodacus tau (Diptera: Tephritidae), including gene 16222 . Protein structure analysis showed that was highly similar odorant binding protein, thus named obp‐Y Obp‐Y knockout (KO) significantly reduced hatching rate offspring. Sperm detection results KO did not affect sperm number testes or transfer during mating. We further examined storage sperms females, found females mating wild‐type began from spermathecal ducts spermathecae at hour 0 after end (AEM), 0–24 h AEM, count gradually increased. However, no were observed mutant hours 0, 4, 8, 24 48 AEM. summary, revealed necessary for females. Our findings only provide theoretical basis elucidating function chromosome, but also offer a molecular target over Z.

Language: Английский

Citations

0