Exosomal miRNAs Differentiate Chronic Total Occlusion from Acute Myocardial Infarction DOI Open Access

Ji-Hye Son,

Jeong‐Kyu Park, Ji Hong Bang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10223 - 10223

Published: Sept. 23, 2024

Although coronary artery occlusion can have a negative effect on the myocardium, chronic total (CTO) exhibits different clinical features from those of acute myocardial infarction (AMI). In this study, we identify differential associations exosomal miRNAs with CTO and AMI. Exosomes were isolated plasma obtained arteries patients undergoing percutaneous intervention to treat (n = 29) AMI 24), followed by small RNA sequencing, target gene predictions, functional enrichment analyses. Promising miRNA markers validated using real-time PCR in 35 CTO, AMI, 10 normal subjects. A 205 detected all subjects, 20 12 upregulated downregulated compared patients, respectively (|fold change| > 4, FDR q < 0.05). The genes that higher associated “regulation cell cycle phase transition”, “cell growth”, “apoptosis”. lower enriched terms such as “muscle differentiation”, “response oxygen levels”, “artery morphogenesis”. On qRT-PCR analysis, expression levels miR-9-5p miR-127-3p significantly between patients. accurately distinguished 79% specificity 97% sensitivity. contents exosomes may play important roles

Language: Английский

Subcellular localization of circular RNAs: Where and why DOI

Susovan Sadhukhan,

Tanvi Sinha, Suchanda Dey

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 715, P. 149937 - 149937

Published: April 17, 2024

Language: Английский

Citations

4
Hsa_circ_0001944 Regulates FXR/TLR4 Pathway and Ferroptosis to Alleviate Nickel Oxide Nanoparticles-Induced Collagen Formation in LX-2 Cells DOI Creative Commons
Haodong Zhou,

Qingyang Chen,

Lijiao Ma

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(4), P. 265 - 265

Published: March 31, 2025

Nickel oxide nanoparticles (NiONPs) can induce liver fibrosis, and their mechanism may be related to non-coding RNA, nuclear receptor signal transduction ferroptosis, but the regulatory relationship between them is not clear. In this study, we aimed investigate role of hsa_circ_0001944 in regulating Farnesol X (FXR)/Toll-like 4 (TLR4) pathway ferroptosis NiONPs-induced collagen deposition. We observed decreased FXR expression, increased TLR4 expression alterations features both rat fibrosis LX-2 cell deposition model. To among FXR, treated cells with agonist (GW4064), inhibitor (TAK-242) (Erastin) combined NiONPs. The results showed that TAK-242 alleviated by increasing features. Furthermore, GW4064 reduced TLR4, indicated inhibited enhanced features, which were involved process induced Subsequently, predicted might regulate through bioinformatics analysis, found NiONPs cells. Overexpression level, summary, demonstrated regulates FXR/TLR4 alleviate formation

Language: Английский

Citations

0
Translational Regulators in Pulmonary Fibrosis: MicroRNAs, Long Non-Coding RNAs, and Transcript Modifications DOI Creative Commons
Sandra Gill, Richard H. Gomer

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 536 - 536

Published: April 3, 2025

Fibrosing disorders including idiopathic pulmonary fibrosis (IPF) are progressive irreversible diseases, often with poor prognoses, characterized by the accumulation of excessive scar tissue and extracellular matrix. Translational regulation has emerged as a critical aspect gene expression control, dysregulation key effectors is associated disease pathogenesis. This review examines current literature on translational regulators in IPF, focusing microRNAs (miRNAs), long non-coding RNAs (lncRNAs), RNA transcript modifications alternative polyadenylation chemical modification. Some these potentiate fibrosis, some inhibit fibrosis. In profibrotic upregulated, antifibrotic downregulated. Correcting defects IPF-associated could be an intriguing avenue for therapeutics.

Language: Английский

Citations

0
Engineering 3D-BMSC exosome-based hydrogels that collaboratively regulate bone microenvironment and promote osteogenesis for enhanced cell‐free bone regeneration DOI Creative Commons

Kai Xiang,

Mu Hao,

Zheng Zhang

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101881 - 101881

Published: May 1, 2025

Language: Английский

Citations

0
Serum miR‑29 is increased in mice with early liver fibrosis DOI Open Access

Kana Matsumoto,

Y Ohsugi,

Chisa Tayama

et al.

Experimental and Therapeutic Medicine, Journal Year: 2024, Volume and Issue: 28(1)

Published: May 15, 2024

Non‑alcoholic steatohepatitis (NASH) is a fatty liver disease that not caused by alcohol consumption and characterized degeneration, inflammation hepatocellular damage. Therefore, predicting future fibrosis critical in the early stages of NASH to prevent progression. The present study examined histological changes as well microRNA (miR/miRNA) expression serum mice model identify potential biomarker candidates could predict fibrosis. This used 6‑week‑old C57BL/6NJcl male fed control with standard solid diet (CE‑2) for breeding propagation groups high‑fat [choline‑deficient 0.1% (w/v) methionine supplemented diet], respectively. Agilent Technologies miRNA microarray was investigate serum. Hematoxylin eosin staining livers group during second week feeding revealed balloon‑like degeneration inflammatory cell infiltration, confirming were state NASH. at 6 weeks showed Microarray analysis miRNAs upregulated 47 downregulated group. Pathway using OmicsNet predicted miR‑29 target collagen genes. Furthermore, NASH‑induced but These findings suggested lower would increase Early suggests leaks from into bloodstream, elevated levels may be predictive

Language: Английский

Citations

3
The circAno6/miR-296-3p/TLR4 signaling axis mediates the inflammatory response to induce the activation of hepatic stellate cells DOI
Zhen Li, Yanzhen Ma, Fan Chang

et al.

Gene, Journal Year: 2024, Volume and Issue: 920, P. 148497 - 148497

Published: April 26, 2024

Language: Английский

Citations

2
Regulation of idiopathic pulmonary fibrosis: a cross-talk between TGF-β signaling and MicroRNAs DOI Creative Commons
Shuo Wang,

Hanming Yu,

Shi Liu

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 25, 2024

Pulmonary fibrosis (PF) is a highly complex and challenging disease affecting the respiratory system. Patients with PF usually have an abbreviated survival period consequential high mortality rate after diagnosis confirmed, posing serious threats to human health. In clinical practice, typically treated by antifibrotic agents, such as Pirfenidone Nintedanib. However, these agents been reported correlate substantial adverse effects, escalating costs, insufficient efficacy. Moreover, it remains unclarified about multifactorial pathology of PF. Therefore, there urgent demand for elucidating underlying mechanisms identifying safe, efficient, targeted therapeutic strategies treatment. The crucial role transforming growth factor- β (TGF-β) signaling pathway in development has explored many studies. MicroRNAs (miRNAs), which function post-transcriptional regulators gene expression, can significantly affect modulating TGF- signaling. turn, TGF-β regulate expression biogenesis miRNAs, thereby substantially progression Hence, that focus on drug-targeted regulation either augmenting down-regulated miRNAs or inhibiting overexpressed may hinder pathways related These contribute prevention suppression provide novel insights into treatment this disease.

Language: Английский

Citations

1
Nucleic acid therapeutics: Past, Present and Future DOI Creative Commons
Sajid Naeem, Ju Zhang, Yang Zhang

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 36(1), P. 102440 - 102440

Published: Dec. 23, 2024

Nucleic acid therapeutics have become increasingly recognized in recent years for their capability to target both coding and non-coding sequences. Several types of nucleic modalities, including siRNA, mRNA, aptamer, along with antisense oligo, been approved by regulatory bodies therapeutic use. The field has brought the forefront rapid development vaccines against COVID-19, followed a number approvals clinical use much anticipated CRISPR-Cas9. However, obstacles such as difficulty achieving efficient targeted delivery diseased sites remain. This review provides an overview highlights substantial advancements, critical engineering, conjugation, strategies, that are paving way growing role modern medicine.

Language: Английский

Citations

1
Mechanobiology in Metabolic Dysfunction-Associated Steatotic Liver Disease and Obesity DOI Creative Commons

Emily L. Rudolph,

LiKang Chin

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(7), P. 7134 - 7146

Published: July 7, 2024

With the ongoing obesity epidemic, prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) is expected to rise and necessitates a greater understanding how proceeds from benign excess lipid in hepatocytes fibrosis eventually cancer. MASLD caused, at least part, by hepatocytes' storage free fatty acids (FAs) that dysfunctional adipocytes are no longer able store, therefore, involves both adipose tissues. The progression not only facilitated biochemical signals, but also mechanical cues such as increase stiffness often seen with fibrotic livers. change accumulation droplets impact ability cell mechanosense mechanotranduce, which perpetuates disease. A mechanosensitive protein largely unexplored could serve potential therapeutic target intermediate filament vimentin. In this review, we briefly summarize recent research on hepatocyte adipocyte mechanobiology provide synopsis studies varied, sometimes contradictory, roles This review intended benefit encourage future context obesity.

Language: Английский

Citations

0
Noncoding RNA-Mediated Epigenetic Regulation in Hepatic Stellate Cells of Liver Fibrosis DOI Creative Commons

Ruoyu Gao,

Jingwei Mao

Non-Coding RNA, Journal Year: 2024, Volume and Issue: 10(4), P. 44 - 44

Published: Aug. 7, 2024

Liver fibrosis is a significant contributor to liver-related disease mortality on global scale. Despite this, there remains dearth of effective therapeutic interventions capable reversing this condition. Consequently, it imperative that we gain comprehensive understanding the underlying mechanisms driving liver fibrosis. In regard, activation hepatic stellate cells (HSCs) recognized as pivotal factor in development and progression The role noncoding RNAs (ncRNAs) epigenetic regulation HSCs transdifferentiation into myofibroblasts has been established, providing new insights gene expression changes during activation. NcRNAs play crucial mediating epigenetics HSCs, serving novel regulators pathogenesis As research expands, connection between ncRNAs involved becomes more evident. These have attracted considerable attention from researchers field. Furthermore, contributed valuable drug discovery identification targets for individuals suffering cirrhosis. such, review offers thorough discussion

Language: Английский

Citations

0