Optofluidic imaging meets deep learning: from merging to emerging DOI
Dickson M. D. Siu, Kelvin C. M. Lee,

Bob M. F. Chung

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(5), P. 1011 - 1033

Published: Jan. 1, 2023

We discuss the recent trends in integrating deep-learning (DL) and optofluidic imaging. A holistic understanding of them could incentivize DL-powered imaging for advancing a wide range novel applications science biomedicine.

Language: Английский

Light-sheets and smart microscopy, an exciting future is dawning DOI Creative Commons
Stephan Daetwyler, Reto Fiolka

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: May 9, 2023

Abstract Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly over long time periods. In this review, we discuss current future developments in light-sheet that expect further expand its capabilities. This includes smart adaptive imaging schemes overcome traditional trade-offs, i.e., spatiotemporal resolution, field of view sample health. microscopy, a microscope will autonomously decide where, when, what how image. We assess image restoration techniques provide avenues these tradeoffs “open top” microscopes may enable multi-modal with high throughput. As such, predict fulfill an important role biomedical clinical the future.

Language: Английский

Citations

52
Two-photon synthetic aperture microscopy for minimally invasive fast 3D imaging of native subcellular behaviors in deep tissue DOI Creative Commons
Zhifeng Zhao, Yiliang Zhou, Bo Liu

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(11), P. 2475 - 2491.e22

Published: May 1, 2023

Holistic understanding of physio-pathological processes requires noninvasive 3D imaging in deep tissue across multiple spatial and temporal scales to link diverse transient subcellular behaviors with long-term physiogenesis. Despite broad applications two-photon microscopy (TPM), there remains an inevitable tradeoff among spatiotemporal resolution, volumes, durations due the point-scanning scheme, accumulated phototoxicity, optical aberrations. Here, we harnessed concept synthetic aperture radar TPM achieve aberration-corrected dynamics at a millisecond scale for over 100,000 large volumes tissue, three orders magnitude reduction photobleaching. With its advantages, identified direct intercellular communications through migrasome generation following traumatic brain injury, visualized formation process germinal center mouse lymph node, characterized heterogeneous cellular states visual cortex, opening up horizon intravital understand organizations functions biological systems holistic level.

Language: Английский

Citations

47
Brillouin microscopy DOI
Irina V. Kabakova, Jitao Zhang, Yuchen Xiang

et al.

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: Feb. 1, 2024

Language: Английский

Citations

24
Electrochemiluminescence Microscopy DOI Creative Commons
Sara Knežević, Dongni Han, Baohong Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(29)

Published: May 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Language: Английский

Citations

23
napari: a Python Multi-Dimensional Image Viewer Platform for the Research Community DOI Open Access

Chi‐Li Chiu,

Nathan Clack

Microscopy and Microanalysis, Journal Year: 2022, Volume and Issue: 28(S1), P. 1576 - 1577

Published: July 22, 2022

Journal Article napari: a Python Multi-Dimensional Image Viewer Platform for the Research Community Get access Chi-Li Chiu, Chiu Chan Zuckerberg Initiative, Redwood City, CA, United States Corresponding author: [email protected] Search other works by this author on: Oxford Academic Google Scholar Nathan Clack, Clack StatesYikes LLC, Baltimore, MD, napari community Microscopy and Microanalysis, Volume 28, Issue S1, 1 August 2022, Pages 1576–1577, https://doi.org/10.1017/S1431927622006328 Published: 01 2022

Language: Английский

Citations

61
Resolution doubling in light-sheet microscopy via oblique plane structured illumination DOI
Bingying Chen, Bo-Jui Chang, Philippe Roudot

et al.

Nature Methods, Journal Year: 2022, Volume and Issue: 19(11), P. 1419 - 1426

Published: Oct. 24, 2022

Language: Английский

Citations

55
Artificial confocal microscopy for deep label-free imaging DOI
Xi Chen, Mikhail E. Kandel, Shenghua He

et al.

Nature Photonics, Journal Year: 2023, Volume and Issue: 17(3), P. 250 - 258

Published: Jan. 12, 2023

Language: Английский

Citations

41
Characterization, comparison, and optimization of lattice light sheets DOI Creative Commons
Gaoxiang Liu, Xiongtao Ruan, Daniel E. Milkie

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(13)

Published: March 31, 2023

Lattice light sheet microscopy excels at the noninvasive imaging of three-dimensional (3D) dynamic processes high spatiotemporal resolution within cells and developing embryos. Recently, several papers have called into question performance lattice sheets relative to Gaussian most common in microscopy. Here, we undertake a theoretical experimental analysis various forms microscopy, which demonstrates explains why provide substantial improvements photobleaching reduction. The provides procedure select correct for desired experiment specifies processing that maximizes use all fluorescence generated excitation envelope optimal while minimizing image artifacts photodamage. We also introduce new type "harmonic balanced" improves spatial frequencies its 3D limits maintains this over lengthened propagation distances allowing expanded fields view.

Language: Английский

Citations

23
Long-term intravital subcellular imaging with confocal scanning light-field microscopy DOI Creative Commons
Zhi Lü,

Siqing Zuo,

Minghui Shi

et al.

Nature Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: May 27, 2024

Abstract Long-term observation of subcellular dynamics in living organisms is limited by background fluorescence originating from tissue scattering or dense labeling. Existing confocal approaches face an inevitable tradeoff among parallelization, resolution and phototoxicity. Here we present scanning light-field microscopy (csLFM), which integrates axially elongated line-confocal illumination with the rolling shutter (sLFM). csLFM enables high-fidelity, high-speed, three-dimensional (3D) imaging at near-diffraction-limit both optical sectioning low By simultaneous 3D excitation detection, intensity can be reduced below 1 mW mm − 2 , 15-fold higher signal-to-background ratio over sLFM. We imaged 25,000 timeframes optically challenging environments different species, such as migrasome delivery mouse spleen, retractosome generation liver voltage Drosophila . Moreover, facilitates large-scale neural recording crosstalk, leading to high orientation selectivity visual stimuli, similar two-photon microscopy, aids understanding coding mechanisms.

Language: Английский

Citations

15
Advanced biological imaging techniques based on metasurfaces DOI Creative Commons
Yongjae Jo, Hyemi Park,

Hyeyoung Yoon

et al.

Opto-Electronic Advances, Journal Year: 2024, Volume and Issue: 0(0), P. 240122 - 240122

Published: Jan. 1, 2024

Language: Английский

Citations

15