Theoretical analysis of low-power deep synergistic sono-optogenetic excitation of neurons by co-expressing light-sensitive and mechano-sensitive ion-channels DOI Creative Commons
Sukhdev Roy,

Gur Pyari,

Himanshu Bansal

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 6, 2025

The present challenge in neuroscience is to non-invasively exercise low-power and high-fidelity control of neurons situated deep inside the brain. Although, two-photon optogenetic excitation can activate millimeter depth with sub-cellular specificity millisecond temporal resolution, it also cause heating targeted tissue. On other hand, sonogenetics modulate cellular activity expressed mechano-sensitive proteins deeper areas brain less spatial selectivity. We a theoretical analysis synergistic sono-optogenetic method overcome these limitations by co-expressing (MscL-I92L) ion-channel light-sensitive (CoChR/ChroME2s/ChRmine) hippocampal neurons. It shown that presence low-amplitude subthreshold ultrasound pulses, threshold for neural spiking reduces drastically 73% MscL-I92L-CoChR (0.021 mW/µm2), 66% MscL-I92L-ChroME2s (0.029 64% MscL-I92L-ChRmine (0.013 mW/µm2) at 5 Hz. allows up 1.2 cm combination. useful design new experiments multimodal neuroprosthetic devices circuits.

Language: Английский

Lights, fiber, action! A primer on in vivo fiber photometry DOI Creative Commons
Eleanor H. Simpson, Thomas Akam, Tommaso Patriarchi

et al.

Neuron, Journal Year: 2023, Volume and Issue: 112(5), P. 718 - 739

Published: Dec. 15, 2023

Fiber photometry is a key technique for characterizing brain-behavior relationships in vivo. Initially, it was primarily used to report calcium dynamics as proxy neural activity via genetically encoded indicators. This generated new insights into brain functions including movement, memory, and motivation at the level of defined circuits cell types. Recently, opportunity discovery with fiber has exploded development an extensive range fluorescent sensors biomolecules neuromodulators peptides that were previously inaccessible critical advance, combined availability affordable "plug-and-play" recording systems, made monitoring molecules high spatiotemporal precision during behavior highly accessible. However, while opening exciting avenues research, rapid expansion applications occurred without coordination or consensus on best practices. Here, we provide comprehensive guide help end-users execute, analyze, suitably interpret studies.

Language: Английский

Citations

50
Triplet–triplet annihilation photon upconversion-mediated photochemical reactions DOI
Ling Huang, Gang Han

Nature Reviews Chemistry, Journal Year: 2024, Volume and Issue: 8(4), P. 238 - 255

Published: March 21, 2024

Language: Английский

Citations

25
Epithelial–mesenchymal transition in tissue repair and degeneration DOI
Khalil Kass Youssef, M. Ángela Nieto

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(9), P. 720 - 739

Published: April 29, 2024

Language: Английский

Citations

25
Neural interfaces: Bridging the brain to the world beyond healthcare DOI Creative Commons
Shumao Xu,

Yang Liu,

Hyun‐Jin Lee

et al.

Exploration, Journal Year: 2024, Volume and Issue: 4(5)

Published: March 14, 2024

Abstract Neural interfaces, emerging at the intersection of neurotechnology and urban planning, promise to transform how we interact with our surroundings communicate. By recording decoding neural signals, these interfaces facilitate direct connections between brain external devices, enabling seamless information exchange shared experiences. Nevertheless, their development is challenged by complexities in materials science, electrochemistry, algorithmic design. Electrophysiological crosstalk mismatch electrode rigidity tissue flexibility further complicate signal fidelity biocompatibility. Recent closed‐loop brain‐computer while promising for mood regulation cognitive enhancement, are limited accuracy adaptability user interfaces. This perspective outlines challenges discusses progress contrasting non‐invasive invasive approaches, explores dynamics stimulation interfacing. Emphasis placed on applications beyond healthcare, highlighting need implantable high‐resolution capabilities.

Language: Английский

Citations

21
Advances in magnetic nanoparticles for molecular medicine DOI Creative Commons
Xiaoyue Yang,

Sarah E. Kubican,

Zhongchao Yi

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Applications of magnetic nanoparticles in medicine have been developed utilizing their imaging capabilities, magnetic–mechanical functions, heating effects, and catalytic properties.

Language: Английский

Citations

2
WiChR, a highly potassium-selective channelrhodopsin for low-light one- and two-photon inhibition of excitable cells DOI Creative Commons
Johannes Vierock,

Enrico Peter,

Christiane Grimm

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(49)

Published: Dec. 9, 2022

The electric excitability of muscle, heart, and brain tissue relies on the precise interplay Na + - K -selective ion channels. involved fluxes are controlled in optogenetic studies using light-gated channelrhodopsins (ChRs). While non-selective cation-conducting ChRs well established for excitation, (KCRs) efficient inhibition have only recently come into reach. Here, we report molecular analysis discovered KCRs from stramenopile Hyphochytrium catenoides identification a novel type hydrophobic selectivity filter. Next, demonstrate that KCR signature motif is conserved related ChRs. Among them, WiChR Wobblia lunata features so far unmatched preference over , stable photocurrents under continuous illumination, prolonged open-state lifetime. Showing high expression levels cardiac myocytes neurons, allows single- two-photon at low irradiance reduced heating. Therefore, recommend as long-awaited versatile inhibitor.

Language: Английский

Citations

56
Structural basis for ion selectivity in potassium-selective channelrhodopsins DOI Creative Commons
Seiya Tajima, Yoon Seok Kim, Masahiro Fukuda

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(20), P. 4325 - 4344.e26

Published: Aug. 30, 2023

KCR channelrhodopsins (K

Citations

32
Illuminating the Brain: Advances and Perspectives in Optoelectronics for Neural Activity Monitoring and Modulation DOI Creative Commons
Shumao Xu,

Marzia Momin,

Salahuddin Ahmed

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(42)

Published: Sept. 19, 2023

Abstract Optogenetic modulation of brain neural activity that combines optical and electrical modes in a unitary system has recently gained robust momentum. Controlling illumination spatial coverage, designing light‐activated modulators, developing wireless light delivery data transmission are crucial for maximizing the use neuromodulation. To this end, biocompatible electrodes with enhanced optoelectrical performance, device integration multiplexed addressing, transmission, multimodal operation soft systems have been developed. This review provides an outlook uniformly illuminating large areas while spatiotemporally imaging responses upon stimulation little artifacts. Representative concepts important breakthroughs, such as head‐mounted illumination, multiple implanted fibers, micro‐light‐delivery devices, discussed. Examples techniques incorporate electrophysiological monitoring presented. Challenges perspectives posed further research efforts toward high‐density interface modulation, potential nonpharmacological neurological disease treatments stimulation.

Language: Английский

Citations

27
How our understanding of memory replay evolves DOI
Zhe Chen,

Matthew A. Wilson

Journal of Neurophysiology, Journal Year: 2023, Volume and Issue: 129(3), P. 552 - 580

Published: Feb. 8, 2023

Memory reactivations and replay, widely reported in the hippocampus cortex across species, have been implicated memory consolidation, planning, spatial skill learning. Technological advances electrophysiology, calcium imaging, human neuroimaging techniques enabled neuroscientists to measure large-scale neural activity with increasing spatiotemporal resolution provided opportunities for developing robust analytic methods identify replay. In this article, we first review a large body of historically important representative replay studies from animal literature. We then discuss our current understanding functions learning, consolidation further progress computational modeling that has contributed these improvements. Next, past present analyses their limitations challenges. Finally, looking ahead, some promising detecting nonstereotypical, behaviorally nondecodable structures recordings. argue seamless integration multisite recordings, real-time decoding, closed-loop manipulation experiments will be essential delineating role wide range cognitive motor functions.

Language: Английский

Citations

25
A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits DOI Creative Commons
Jonas Wietek,

Adrianna Nozownik,

Mauro Pulin

et al.

Nature Methods, Journal Year: 2024, Volume and Issue: 21(7), P. 1275 - 1287

Published: May 29, 2024

Abstract Information is transmitted between brain regions through the release of neurotransmitters from long-range projecting axons. Understanding how activity such connections contributes to behavior requires efficient methods for reversibly manipulating their function. Chemogenetic and optogenetic tools, acting endogenous G-protein-coupled receptor pathways, can be used modulate synaptic transmission, but existing tools are limited in sensitivity, spatiotemporal precision or spectral multiplexing capabilities. Here we systematically evaluated multiple bistable opsins applications found that Platynereis dumerilii ciliary opsin ( Pd CO) an efficient, versatile, light-activated suppress transmission mammalian neurons with high temporal vivo. CO has useful biophysical properties enable other actuators reporters. We demonstrate conduct reversible loss-of-function experiments projections behaving animals, thereby enabling detailed synapse-specific functional circuit mapping.

Language: Английский

Citations

15