Highly sensitive label-free biosensor: graphene/CaF2 multilayer for gas, cancer, virus, and diabetes detection with enhanced quality factor and figure of merit

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 27, 2023

Abstract One of the primary goals for researchers is to create a high-quality sensor with simple structure because urgent requirement identify biomolecules at low concentrations diagnose diseases and detect hazardous chemicals health early on. Recently graphene has attracted much interest in field improved biosensors. Meanwhile, new materials such as CaF 2 been widely used improve applications graphene-based sensors. Using fantastic features graphene/CaF multilayer, this article proposes an improvement sensitivity (S), figure merit (FOM), quality factor (Q). The proposed based on five-layers graphene/dielectric grating integrated Fabry–Perot cavity. By tuning chemical potential (µ c ), due semi-metal graphene, surface plasmon resonance (SPR) waves excited boundaries. Due vertical polarization source gratings symmetry electric field, both corners act dipoles, causes propagation plasmonic propagate towards each other. Finally, it (FP) interference structure's active medium (the area where sample located). In article, using inherent nature FP its S environment's refractive index (RI), by changing minimal amount RI sample, wavelength (interferometer order) shifts sharply. design can sense some cancers, Adrenal Gland Cancer, Blood Breast Cancer I, II, Cervical skin cancer precisely. optimizing structure, we achieve high 9000 nm/RIU FOM about 52.14 first order (M 1 ). Likewise, remarkable 38,000 81 have obtained second mode addition, label-free SPR changes concentration various materials, including gases biomolecules, hemoglobin, breast cancer, diabetes, leukemia, most alloys, accuracy 0.001. urine maximum 8500 cancers 6000 range 7000 nm/RIU. Also, four viruses, M13 bacteriophage, HIV type one, Herpes simplex 1, influenza, investigated, showing Maximum (for λ R ) 8000 (λ = 11.2 µm), 12,000 10.73 11.78 10.6 respectively, )) mentioned viruses are 4740 8.7 8010 8.44 8100 10.15 8.36 respectively.

Language: Английский

---

A review of the recent achievements and future trends on 3D printed microfluidic devices for bioanalytical applications

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1299, P. 342429 - 342429

Published: Feb. 28, 2024

Language: Английский

---

Epidemiology, mechanisms, and clinical impact of bacterial heteroresistance

npj Antimicrobials and Resistance, Journal Year: 2025, Volume and Issue: 3(1)

Published: Jan. 28, 2025

Bacterial heteroresistance, a phenomenon where subpopulations within bacterial strain exhibit significantly reduced antibiotic susceptibility compared to the main population, poses major challenge in managing infectious diseases. It is considered an intermediate stage evolution of bacteria towards full resistance. Heteroresistant strains often have minimal inhibitory concentration (MIC) that appears sensitive, making detection and differentiation clinical settings difficult. As result, impact on outcomes challenging fully understand, as it remains "hidden". In recent years, heteroresistance has received increasing attention. However, still poorly understood underappreciated. We provide overview epidemiology, mechanisms, heteroresistance. This review underscores critical importance understanding addressing ongoing fight against resistance

Language: Английский

---

Gold Nanoparticle-Based Colorimetric Biosensing for Foodborne Pathogen Detection

Foods, Journal Year: 2023, Volume and Issue: 13(1), P. 95 - 95

Published: Dec. 27, 2023

Ensuring safe high-quality food is an ongoing priority, yet consumers face heightened risk from foodborne pathogens due to extended supply chains and climate change in the industry. Nanomaterial-based assays are popular have recently been developed ensure safety high quality. This review discusses strategies for utilizing gold nanoparticles colorimetric biosensors. The visible-signal biosensor proves be a potent sensing technique directly measuring targets related field of analysis. Among biosensors, localized surface plasmon resonance (LSPR) has garnered increasing attention experienced rapid development recent years. succinctly introduces origin LSPR theory, providing detailed insights into its fundamental principles. Additionally, this delves application nanotechnology implementation biosensor, exploring methods elucidating factors that influence generation visible signals. Several emerging technologies aimed at simple immunoassays onsite applications introduced In foreseeable future, field-friendly biosensors could adopted monitoring systems. real-time detection possible contaminants biological substances water essential human health safety.

Language: Английский

---

Propagation, Conservation and Improvement of Industrial Crops Through Tissue Culture

Published: Jan. 1, 2025

Language: Английский

---

Precision single cell analysis to characterize host dependent antimicrobial response heterogeneity in physiological medium

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

We highlight the interindividual heterogeneity in pathogen killing kinetics by physiological concentrations of antibiotics urine and develop a microfluidic device capable tracking single-cell trajectories while allowing for reagent exchange.

Language: Английский

---

Dynamics of non-Newtonian agarose gel droplet formation in two-phase microfluidic systems

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(3)

Published: March 1, 2025

Droplet-based microfluidics is a valuable tool in interdisciplinary research fields like cell biology and diagnostics. Newtonian fluids, aqueous-based solutions, are commonly used for droplet generation. However, non-Newtonian e.g., hydrogels, becoming increasingly popular as the dispersed phase. In this study, we investigate dynamics of ultra-low-gelling agarose formation under different conditions to evaluate stability, with an aim better understand underlying physics formation. We varied gel concentration, temperature (40, 50, 60 °C), flow rate ratio (ϕ) between continuous phase observed squeezing regime (capillary number, Cac < 0.015) T-junction conditions. experimentally investigated size (LD/w) function those four parameters found that LD/w depends strongly on ϕ, (which affects viscosity ratio, λ), but only weakly dependent Cac. then confirmed our experimental findings numerical simulations, which showed good agreement across all numerically process consists five stages, namely, filling, necking, pinching, threading, breakup, where threading additional stage Finally, simulation, concluded length (lthread) directly proportional ϕ has complex relation temperature.

Language: Английский

---

Nanotechnology in Gene Editing: Pioneering CRISPR‐Cas Delivery Systems to Tackle Antibiotic Resistance

Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract The rise of antibiotic‐resistant bacteria, driven by antibiotic misuse, is a major global health threat. Addressing this issue requires understanding resistance mechanisms and developing innovative solutions. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐associated systems (Cas), genome‐editing tool derived from prokaryotic defense mechanisms, offers precise targeting genes. By reprogramming CRISPR‐Cas, bacteria can be killed or resensitized to antibiotics through plasmid curing. However, clinical applications face challenges, particularly in delivering CRISPR‐Cas components effectively. Nanotechnology has emerged as promising approach for targeted delivery tissues cells. This paper explores the molecular resistance, emphasizing structure function their mechanisms. It highlights use nanoparticles (NPs) nanoscale carriers deliver components, reviewing recent studies that combine NPs CRISPR target Additionally, discusses current challenges future prospects field, underscoring potential nanotechnology combat resistance.

Language: Английский

---

A tube-based biosensor for DNA and RNA detection

Science Advances, Journal Year: 2025, Volume and Issue: 11(18)

Published: May 2, 2025

Affordable, sensitive, and simplified DNA/RNA detection is important for disease diagnosis enables timely medical intervention measures. Usually, high sensitivity depends on expensive instruments sophisticated procedures, making contradict affordability simplicity. Here, we proposed an ultra-sensitive single-tube biosensor (USTB) where users can visually detect targets by observing the liquid motion state in a glass tube. The developed instrument-free USTB performed low-cost ($0.1), fast (1 min), both fragments (≤1 aM) clinical positive samples, which commercial reverse transcription polymerase chain reaction (RT-PCR) PCR kits could not effectively recognize. Furthermore, promising to be easily applied other-type biomarkers designed smart sensing unit.

Language: Английский

---

Single-Bacterium Diagnosis via Terahertz Near-Field Dielectric Nanoimaging

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Single-bacterium diagnostic methods with unprecedented precision and rapid turnaround times are promising tools for facilitating the transition from empirical treatment to personalized anti-infection treatment. Terahertz (THz) radiation, a cutting-edge technology identifying pathogens, enables label-free non-destructive detection of intermolecular vibrational modes bacterial dielectric properties. However, this individual property-based mismatched spatial resolution limited single-bacterium identification various species pathogens. Here, we demonstrate THz nanoimaging (STDN) strategy by customized scattering-type scanning near-field optical microscopy. The nanoimages bacteria explained confirmed theoretical modeling measurement. By synchronously tracking intrinsic property extrinsic morphology, achieved 99.3% 91.6% accuracy in antibiotic susceptibility testing trained classifier within 2 hours. This proof-of-concept STDN may propel precise infection management help counteract resistance.

Language: Английский

---