An integrated multi-omics analysis reveals osteokines involved in global regulation

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(5), P. 1144 - 1163.e7

Published: April 3, 2024

Language: Английский

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LXR/CD38 activation drives cholesterol-induced macrophage senescence and neurodegeneration via NAD+ depletion

Cell Reports, Journal Year: 2024, Volume and Issue: 43(5), P. 114102 - 114102

Published: April 17, 2024

Although dysregulated cholesterol metabolism predisposes aging tissues to inflammation and a plethora of diseases, the underlying molecular mechanism remains poorly defined. Here, we show that metabolic genotoxic stresses, convergently acting through liver X nuclear receptor, upregulate CD38 promote lysosomal efflux, leading nicotinamide adenine dinucleotide (NAD

Language: Английский

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Proteomic analysis of egg production peak and senescence in the ovaries of Taihe black-boned silky fowl (Gallus gallus domesticus Brisson)

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 7, 2025

The Taihe black-boned silky fowl, a distinguished indigenous breed of chicken, is renowned for its dual utility in both traditional medicinal and culinary applications. However, the faces significant challenges due to suboptimal reproductive capabilities notably brief egg-laying period, which have impeded broader development cultivation. In this research endeavor, we employed an advanced, rapid DIA (Data independent acquisition) quantitative proteomics method on Astral platform meticulously analyze ovarian proteome these chickens. By analyzing proteomic information fowl during peak decline periods, aim identify potential candidate proteins molecular mechanisms underlying decline. This could enable us implement interventions improve efficiency valuable breed. study, total 8,281 were identified within fowl. Among these, 303 exhibited differential expression, with 98 significantly up-regulated 205 down-regulated. functional annotation illuminated their crucial roles steroid hormone synthesis pathways, are pivotal egg production. Furthermore, later stages laying, there was noticeable upregulation associated inflammatory senescence oxidative stress. change suggests increase stress ovary, highlighting physiological shifts that affect productivity as chickens age. study key protein markers critical phases cycle, specifically late periods. These findings contribute new scientific insights can be utilized breeding optimization effective management unique elucidating dynamics different laying phases, offers strategies aimed at enhancing performance extending lifespan lead improvements sustainability profitability farming

Language: Английский

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Ferroptosis-related genes participate in the microglia-induced neuroinflammation of spinal cord injury via NF-κB signaling: evidence from integrated single-cell and spatial transcriptomic analysis

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 11, 2025

Ferroptosis and immune responses are critical pathological events in spinal cord injury (SCI), whereas relative molecular cellular mechanisms remain unclear. Micro-array datasets (GSE45006, GSE69334), RNA sequencing (RNA-seq) dataset (GSE151371), spatial transcriptome (GSE214349, GSE184369), single cell (scRNA-seq) (GSE162610, GSE226286) were available from the Gene Expression Omnibus (GEO) database. Through weighted gene co-expression network analysis differential expression GSE45006, we identified differentially expressed time- immune-related genes (DETIRGs) associated with chronic SCI ferroptosis- (DEFIRGs), which validated GSE151371. Protein-protein interaction microRNA-mRNA-transcription factor regulatory networks constructed based on Search Tool for Retrieval of Interacting Genes (STRING) NetworkAnalyst, respectively, by chromatin immunoprecipitation followed (ChIP-seq). Cell subclusters unique features microglia single-cell transcriptomic analysis, GSE226286. Spatial patterns DETIRGs DEFIRGs brain (GSE214349) (GSE184369). Potential underlying neuronal regeneration neurotrophin-3 (NT3)-chitosan revealed analyses GSE69334. Immune- ferroptosis-related nanolayered double hydroxide loaded NT3 (LDH-NT3) investigated vivo vitro. GBP2, TEC, UNC93B1, PLXNC1, NFATC1, IL10RB, TLR8 represented SCI-specific peripheral blood biomarkers. NFKB1 may regulate CYBB HMOX1 a subcluster M1 within middle lesion, establishing links between microglial ferroptosis neuroinflammation. Reduced inflammatory potential effects NT3-chitosan or LDH-NT3 regeneration. A novel exhibiting polarization phenotype was involved SCI. These trigger neuroinflammation induce degeneration site SCI, might be inhibited LDH-NT3.

Language: Английский

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Hallmarks of ovarian aging

Trends in Endocrinology and Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Multidimensional profiling of human T cells reveals high CD38 expression, marking recent thymic emigrants and age-related naive T cell remodeling

Immunity, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

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Harnessing omics data for drug discovery and development in ovarian aging

Human Reproduction Update, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Ovarian aging occurs earlier than the of many other organs and has a lasting impact on women's overall health well-being. However, effective interventions to slow ovarian remain limited, primarily due an incomplete understanding underlying molecular mechanisms drug targets. Recent advances in omics data resources, combined with innovative computational tools, are offering deeper insight into complexities aging, paving way for new opportunities discovery development. This review aims synthesize expanding multi-omics data, spanning genome, transcriptome, proteome, metabolome, microbiome, related from both tissue-level single-cell perspectives. We will specially explore how analysis these emerging datasets can be leveraged identify novel targets guide therapeutic strategies slowing reversing aging. conducted comprehensive literature search PubMed database using range relevant keywords: age at natural menopause, premature insufficiency (POI), diminished reserve (DOR), genomics, transcriptomics, epigenomics, DNA methylation, RNA modification, histone proteomics, metabolomics, lipidomics, single-cell, genome-wide association studies (GWAS), whole-exome sequencing, phenome-wide (PheWAS), Mendelian randomization (MR), epigenetic target, machine learning, artificial intelligence (AI), deep multi-omics. The was restricted English-language articles published up September 2024. Multi-omics have uncovered key driving including damage repair deficiencies, inflammatory immune responses, mitochondrial dysfunction, cell death. By integrating researchers critical regulatory factors across various biological levels, leading potential Notable examples include genetic such as BRCA2 TERT, like Tet FTO, metabolic sirtuins CD38+, protein BIN2 PDGF-BB, transcription FOXP1. advent cutting-edge technologies, especially technologies spatial provided valuable insights guiding treatment decisions become powerful tool aimed mitigating or As technology advances, integration AI models holds more accurately predict candidate convergence offers promising avenues personalized medicine precision therapies, tailored Not applicable.

Language: Английский

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Exploring the heterogeneous targets of metabolic aging at single-cell resolution

Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Language: Английский

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A Molecular Perspective and Role of NAD+ in Ovarian Aging

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4680 - 4680

Published: April 25, 2024

The decline in female fecundity is linked to advancing chronological age. ovarian reserve diminishes quantity and quality as women age, impacting reproductive efficiency the aging process rest of body. NAD+ an essential coenzyme cellular energy production, metabolism, cell signaling, survival. It involved various age-related conditions. Hallmarks associated with aging, diseases, metabolic dysfunctions can significantly affect fertility by disturbing delicate relationship between metabolism reproduction. Enzymes such sirtuins, PARPs, CD38 play roles biology, which actively consume their enzymatic activities. In recent years, has gained much attention for its role diseases like cancer, Alzheimer’s, cardiovascular neurodegenerative disorders, highlighting involvement pathophysiological processes. However, impact on reproduction not well understood. This review aims bridge this knowledge gap comprehensively exploring complex interplay biology providing valuable information that could help develop plans improve women’s health prevent issues.

Language: Английский

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Fertility protection during chemotherapy treatment by boosting the NAD(P)+ metabolome

EMBO Molecular Medicine, Journal Year: 2024, Volume and Issue: 16(10), P. 2583 - 2618

Published: Aug. 21, 2024

Language: Английский

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