Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 837 - 837

Published: Aug. 23, 2023

Lateral flow tests are one of the most important types paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving rapid screening and management infections in global pandemics or other health disorders by using minimally expert staff locations where no sophisticated laboratory services accessible. They can detect different biomarkers various biological samples provide results a little time at low price. An challenge regarding conventional LFAs is increasing their sensitivity specificity. There two main approaches to increase specificity, including assay improvement target enrichment. Assay comprises optimization signal amplification techniques. In this study, summarize specificity enhancement strategies with objective evaluation presented, such detection element immobilization, capillary rate adjusting, label evolution, sample extraction enrichment, etc. also key findings LFA performance solving limitations discussed along numerous examples.

Language: Английский

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Recent advances in point-of-care testing of COVID-19

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(24), P. 8500 - 8530

Published: Jan. 1, 2023

This review explores various point-of-care optical diagnostic systems combined with microdevices developed during the recent COVID-19 pandemic for clinical diagnostics.

Language: Английский

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Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

ACS Nano, Journal Year: 2024, Volume and Issue: 18(3), P. 1757 - 1777

Published: Jan. 8, 2024

Many systems have been designed for the detection of SARS-CoV-2, which is virus that causes COVID-19. SARS-CoV-2 readily transmitted, resulting in rapid spread disease human populations. Frequent testing at point care (POC) a key aspect controlling outbreaks caused by and other emerging pathogens, as early identification infected individuals can then be followed appropriate measures isolation or treatment, maximizing chances recovery preventing infectious spread. Diagnostic tools used high-frequency should inexpensive, provide diagnostic response without sophisticated equipment, amenable to manufacturing on large scale. The application these devices enable large-scale data collection, help control viral transmission, prevent propagation. Here we review functional nanomaterial-based optical electrochemical biosensors accessible POC These incorporate nanomaterials coupled with paper-based analytical inexpensive substrates, traditional lateral flow technology (antigen antibody immunoassays), innovative biosensing methods. We critically discuss advantages disadvantages nanobiosensor-based approaches compared widely technologies such PCR, ELISA, LAMP. Moreover, delineate main technological, (bio)chemical, translational, regulatory challenges associated developing reliable biosensors, prevented their translation into clinic. Finally, highlight how nanobiosensors, given unique over existing tests, may future pandemics.

Language: Английский

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Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(12), P. 4783 - 4790

Published: March 12, 2024

The 2019 coronavirus disease (COVID-19) outbreak created an unprecedented need for rapid, sensitive, and cost-effective point-of-care diagnostic tests to prevent mitigate the spread of SARS-CoV-2 virus. Herein, we demonstrated advanced lateral flow immunoassay (LFIA) platform with dual-functional [colorimetric surface-enhanced Raman scattering (SERS)] detection spike 1 (S1) protein SARS-CoV-2. nanosensor was integrated a specially designed core–gap–shell morphology consisting gold shell decorated external nanospheres, structure referred as nanocrown (GNC), labeled reporter molecule 1,3,3,1′,3′,3′-hexamethyl-2,2′-indotricarbocyanine iodide (HITC) produce strong colorimetric signal well enhanced SERS signal. Among different plasmonics-active GNC nanostructures, GNC-2 morphology, which has optimum number size produces intense dark-blue ultrahigh limit (LOD) S1 via LFIA determined be 91.24 pg/mL. On other hand, LOD method more than three orders magnitude lower at 57.21 fg/mL. Furthermore, analyzed performance directly analyzing spiked in saliva samples without any sample pretreatment achieving low 39.65 fg/mL using SERS-based plasmonics-enhanced LFIA, indicating sensitivity. Overall, our showed excellent sensitivity, reproducibility, rapid protein, demonstrating potential promising early respiratory virus infections.

Language: Английский

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Rapid deep learning-assisted predictive diagnostics for point-of-care testing

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 24, 2024

Prominent techniques such as real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and rapid kits are currently being explored to both enhance sensitivity reduce time for diagnostic tests. Existing commercial molecular methods typically take several hours, while immunoassays can range from hours tens of minutes. Rapid diagnostics crucial in Point-of-Care Testing (POCT). We propose an approach that integrates a time-series deep learning architecture AI-based verification, the enhanced result analysis lateral flow assays. This is applicable infectious diseases non-infectious biomarkers. In blind tests using clinical samples, our method achieved times short 2 minutes, exceeding accuracy human at 15 Furthermore, technique significantly reduces just 1-2 minutes POCT setting. advancement has potential greatly diagnostics, enabling healthcare professionals non-experts make rapid, accurate decisions.

Language: Английский

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Sensors for Emerging Water Contaminants: Overcoming Roadblocks to Innovation

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(6), P. 2636 - 2651

Published: Feb. 1, 2024

Ensuring water quality and safety requires the effective detection of emerging contaminants, which present significant risks to both human health environment. Field deployable low-cost sensors provide solutions detect contaminants at their source enable large-scale monitoring management. Unfortunately, availability utilization such remain limited. This Perspective examines current sensing technologies for detecting analyzes critical barriers, as high costs, lack reliability, difficulties in implementation real-world settings, stakeholder involvement sensor design. These technical nontechnical barriers severely hinder progression from proof-of-concepts negatively impact user experience factors ease-of-use actionability using data, ultimately affecting successful translation widespread adoption these technologies. We examples specific systems explore key strategies address remaining scientific challenges that must be overcome translate into field improving sensitivity, selectivity, robustness, performance environments. Other aspects tailoring research meet end-users' requirements, integrating cost considerations consumer needs early prototype design, establishing standardized evaluation validation protocols, fostering academia-industry collaborations, maximizing data value by sharing initiatives, promoting workforce development are also discussed. The describes a set guidelines development, translation, swiftly accurately detect, analyze, track, manage contamination.

Language: Английский

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Nanoparticle‐Based Bioaffinity Assays: From the Research Laboratory to the Market

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(3)

Published: Dec. 2, 2023

Abstract Advances in the development of new biorecognition elements, nanoparticle‐based labels as well instrumentation have inspired design bioaffinity assays. This review critically discusses potential nanoparticles to replace current enzymatic or molecular immunoassays and other Successful implementations commercial assays need for rapid tests incorporating different roles such capture support, signal generation amplification systems are highlighted. The limited number applied can be explained by challenges associated with analysis real samples (e.g., blood, urine, nasal swabs) that difficult resolve, particularly if same performance achieved more easily conventional labels. Lateral flow based on visual detection red‐colored line formed colloidal gold a notable exception, exemplified SARS‐CoV‐2 antigen moved from initial laboratory testing widespread market adaption less than two years.

Language: Английский

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Deep Learning‐Enabled Multiplexed Point‐of‐Care Sensor using a Paper‐Based Fluorescence Vertical Flow Assay

Small, Journal Year: 2023, Volume and Issue: 19(51)

Published: April 27, 2023

Abstract Multiplexed computational sensing with a point‐of‐care serodiagnosis assay to simultaneously quantify three biomarkers of acute cardiac injury is demonstrated. This sensor includes paper‐based fluorescence vertical flow (fxVFA) processed by low‐cost mobile reader, which quantifies the target through trained neural networks, all within <15 min test time using 50 µL serum sample per patient. fxVFA platform validated human samples biomarkers, i.e., myoglobin, creatine kinase‐MB, and heart‐type fatty acid binding protein, achieving less than 0.52 ng mL −1 limit‐of‐detection for minimal cross‐reactivity. Biomarker concentration quantification that coupled network‐based inference blindly tested 46 individually activated cartridges, shows high correlation ground truth concentrations >0.9 linearity <15% coefficient variation. The competitive performance this multiplexed along its inexpensive design handheld footprint makes it promising can expand access diagnostics in resource‐limited settings.

Language: Английский

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Self-calibrating surface-enhanced Raman scattering-lateral flow immunoassay for determination of amyloid-β biomarker of Alzheimer's disease

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 245, P. 115840 - 115840

Published: Nov. 16, 2023

Language: Английский

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Weakly ionized gold nanoparticles amplify immunoassays for ultrasensitive point-of-care sensors

Science Advances, Journal Year: 2024, Volume and Issue: 10(28)

Published: July 10, 2024

Gold nanoparticle-based lateral flow immunoassays (AuNP LFIAs) are widely used point-of-care (POC) sensors for in vitro diagnostics. However, the sensitivity limitation of conventional AuNP LFIAs impedes detection trace biomarkers. Several studies have explored size and shape factors AuNPs derivative nanohybrids, showing limited improvements or enhanced at cost convenience affordability. Here, we investigated surface chemistry on LFIAs. By modifying ligands, a strategy involving weakly ionized enables ultrasensitive naked-eye (~100-fold sensitivity). We demonstrated how this chemistry-amplified immunoassay approach modulates nanointerfacial bindings to promote antibody adsorption higher activity adsorbed antibodies. This design eliminates complex nanosynthesis, auxiliary devices, additional reagents while efficiently improving with advantages: simplified fabrication process, excellent reproducibility reliability, ultrasensitivity toward various The using represents versatile sensitizing POC sensors.

Language: Английский

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