Ferromagnetic Resonance Multiplexing of Orthogonal Magnetic Nanoparticle Agglutination for Simultaneous Analysis of Nucleic Acid Mutations and Nonspecific Interactions DOI
Xiaoyan Zhang, Yuan Fang, Zhenhua Chen

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Developing multiplexed platforms capable of simultaneously analyzing specific biomolecular interactions and nonspecific binding effects in a single reaction system holds significant potential for accurate disease diagnostics. Here, ferromagnetic resonance (FMR) biosensor is presented that integrates orthogonal magnetic nanoparticle (MNP) agglutination with genetic algorithm (GA)‐based spectral deconvolution. Different Mycobacterium tuberculosis drug‐resistance mutations can induce reactions including padlock probe ligation, rolling circle amplification, MNP agglutination, producing homogeneous assemblies. Meanwhile, heterogeneous assemblies form due to interactions. FMR spectrum the mixture deconvoluted into three components, each representing rifampicin‐resistant single‐nucleotide mutation rpoB 531T, isoniazid‐resistant katG 315C inhA −15T, total effect suspension. The method demonstrates detection limits as low 50 f m target sequences within 80 min, validated by wild‐type sequences, 5% serum samples, clinical sputum samples. This triplex analysis not only enhances accuracy but also enables real‐time error correction, which feature unattainable conventional duplex assays.

Language: Английский

Small-molecule probes from bench to bedside: advancing molecular analysis of drug–target interactions toward precision medicine DOI
Sijun Pan, Aixiang Ding, Yisi Li

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(16), P. 5706 - 5743

Published: Jan. 1, 2023

Integrated development of designer chemical tools and complementary analytical technologies empowers new scientific clinical opportunities.

Language: Английский

Citations

33
Multiplexed RNA profiling by regenerative catalysis enables blood-based subtyping of brain tumors DOI Creative Commons
Yan Zhang, Chi Yan Wong,

Carine Z. J. Lim

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 17, 2023

Current technologies to subtype glioblastoma (GBM), the most lethal brain tumor, require highly invasive biopsies. Here, we develop a dedicated analytical platform achieve direct and multiplexed profiling of circulating RNAs in extracellular vesicles for blood-based GBM characterization. The technology, termed 'enzyme ZIF-8 complexes regenerative catalytic digital detection RNA' (EZ-READ), leverages an RNA-responsive transducer regeneratively convert catalytically enhance signals from rare RNA targets. Each comprises hybrid - protein enzymes encapsulated within metal organic frameworks configure strong activity robust protection. Upon target hybridization, activates directly liberate complexes, target-recyclable manner; when partitioned microfluidic device, these can individually catalyze chemifluorescence reactions quantification. EZ-READ thus enables programmable reliable detection, across different-sized subtypes (miRNA mRNA), sample lysates. When clinically evaluated, established composite signatures accurate diagnosis subtyping.

Language: Английский

Citations

27
Label-Free and Rapid Microfluidic Design Rules for Circulating Tumor Cell Enrichment and Isolation: A Review and Simulation Analysis DOI Creative Commons
Muhammad Asraf Mansor, Chun Yang,

Kar Lok Chong

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(7), P. 6306 - 6322

Published: Feb. 11, 2025

Enriching and isolating circulating tumor cells (CTCs) have attracted significant interest due to their important role in early cancer diagnosis prognosis, allowing for minimally invasive approaches providing vital information about metastasis at the cellular level. This review comprehensively summarizes recent developments microfluidic devices CTC enrichment isolation. The advantages limitations of several are discussed, design specifications highlighted. We also developed a set methodologies rules label-free microfluidics such as spiral, deterministic lateral displacement (DLD) dielectrophoresis (DEP) allow researchers develop systematically effectively, promoting rapid research on design, fabrication, experimentation.

Language: Английский

Citations

1
The Potential of cfDNA as Biomarker: Opportunities and Challenges for Neurodegenerative Diseases DOI Creative Commons
Şeyma Aydın, Selçuk Özdemir, Ahmet Adıgüzel

et al.

Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(1)

Published: March 13, 2025

Abstract Neurodegenerative disorders, including Alzheimer’s disease (AD), Parkinson’s (PD), multiple sclerosis (MS), and amyotrophic lateral (ALS), are characterized by the progressive gradual degeneration of neurons. The prevalence rates these disorders rise significantly with age. As life spans continue to increase in many countries, number cases is expected grow foreseeable future. Early precise diagnosis, along appropriate surveillance, continues pose a challenge. high heterogeneity neurodegenerative diseases calls for more accurate definitive biomarkers improve clinical therapy. Cell-free DNA (cfDNA), fragmented released into bodily fluids via apoptosis, necrosis, or active secretion, has emerged as promising non-invasive diagnostic tool various diseases. cfDNA can serve an indicator ongoing cellular damage mortality, neuronal loss, may provide valuable insights processes, progression, therapeutic responses. This review will first cover key aspects then examine recent advances its potential use biomarker disorders.

Language: Английский

Citations

1
Magnetic particles-integrated CRISPR/Cas systems for biosensing DOI
Zhilong Wang,

Luyu Wei,

Yiping Chen

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 171, P. 117525 - 117525

Published: Jan. 4, 2024

Language: Английский

Citations

6
Colloidal quantum dots-modified electrochemical sensor for high-sensitive extracellular vesicle detection DOI
Jing Huang, Tucan Chen, Yunong Zhao

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 487, P. 150616 - 150616

Published: March 21, 2024

Language: Английский

Citations

6
Paper-Based Analytical Devices for Cancer Liquid Biopsy DOI
Sima Singh, Ada Raucci,

Wanda Cimmino

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(9), P. 3698 - 3706

Published: Feb. 20, 2024

Liquid biopsies have caused a significant revolution in cancer diagnosis, and the use of point care (PoC) platforms has potential to bring liquid biopsy-based detection closer patients. These provide rapid on-site analysis by reducing time between sample collection results output. The aim this tutorial content is readers an in-depth understanding regarding choice ideal sensing platform suitable for specific cancer-related biomarkers.

Language: Английский

Citations

5
Aptamer biorecognition and gold nanoshell-mediated fluorescence quenching for glycoprotein analysis and breast cancer diagnosis DOI
Xingjie Wu,

Hong Liu,

Jialin Chen

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 201, P. 110719 - 110719

Published: May 8, 2024

Language: Английский

Citations

5
High-Efficiency Enzyme Assay and Screening of Enzyme-Inhibiting Nanomaterials Using Capillary Electrophoresis with Hierarchically Porous Metal–Organic Framework-Based Immobilized Enzyme Microreactor DOI

Meiting Yan,

Dan Wang,

Hongyan Liao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17300 - 17309

Published: Oct. 16, 2024

Enzyme-inhibiting nanomaterials have significant potential for regulating enzyme activity. However, a universal and efficient method systematically screening evaluating the inhibitory effects of various on drug target enzymes has not been established. While integrated technique immobilized microreactor (IMER) with capillary electrophoresis (CE) serves as an effective tool analysis, it still faces challenges such low loadability, unsatisfactory stability, limited applicability. Herein, hierarchical porous metal–organic frameworks (HP-MOFs) were explored high-performance immobilization carriers stationary phases to develop novel HP-MOFs-based IMER-CE microanalysis system online assay systematic enzyme-inhibiting nanomaterials. As proof-of-concept demonstration, model xanthine oxidase (XOD) was HP-UiO-66-NH2 coated capillary, serving durable IMER XOD-inhibiting The hierarchically micro- mesoporous structure superior loadability as-prepared HP-UiO-66-NH2–IMER intensively characterized, followed by evaluation separation performance column kinetics XOD. Compared microporous UiO-66-NH2–IMER, HP-UiO-66-NH2–IMER-CE showed improvements in loading, maximum reaction rate, repeatability, long-term stability. Furthermore, established effectively employed screen XOD activity nanomaterials, revealing that graphene oxide, single wall carbon nanotube three other exhibited potentials. can be easily expanded modifying types holds accelerate identification rational design

Language: Английский

Citations

5
Electrochemical Biosensors for Cancer Diagnosis: Multitarget Analysis to Present Molecular Characteristics of Tumor Heterogeneity DOI Creative Commons
Ya Cao, Jianan Xia, Lijuan Li

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(12), P. 4655 - 4672

Published: Dec. 11, 2024

Electrochemical biosensors are gaining attention as powerful tools in cancer diagnosis, particularly liquid biopsy, due to their high efficiency, rapid response, exceptional sensitivity, and specificity. However, the complexity of intra- intertumor heterogeneity, with variations genetic protein expression profiles epigenetic modifications, makes electrochemical susceptible false-positive or false-negative diagnostic outcomes. To address this challenge, there is growing interest simultaneously analyzing multiple biomarkers reveal molecular characteristics tumor heterogeneity for precise diagnosis. In Perspective, we highlight recent advancements utilizing a specific emphasis on multitarget analysis including tumor-associated nucleic acids, markers, extracellular vesicles, cells. These hold significant promise improving precision early diagnosis monitoring, well potentially offering new insights into personalized management.

Language: Английский

Citations

5