High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 14, 2024

Abstract Cytosine base editors (CBEs) and adenine (ABEs) enable precise C-to-T A-to-G edits. Recently, ABE8e, derived from TadA-8e, enhances edits in mammalian cells plants. Interestingly, TadA-8e can also be evolved to confer editing. This study compares engineered CBEs rice tomato cells, identifying TadCBEa, TadCBEd, TadCBEd_V106W as efficient with high purity a narrow editing window. A dual editor, TadDE, promotes simultaneous Multiplexed TadCBEa TadDE is demonstrated transgenic rice, no off-target effects detected by whole genome transcriptome sequencing, indicating specificity. Finally, two crop engineering applications using are shown: introducing herbicide resistance alleles OsALS creating synonymous mutations OsSPL14 resist OsMIR156 -mediated degradation. Together, this presents editor valuable additions the plant toolbox.

Language: Английский

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Crop Improvement: Comparison of Transgenesis and Gene Editing

Horticulturae, Journal Year: 2024, Volume and Issue: 10(1), P. 57 - 57

Published: Jan. 6, 2024

The development and improvement of molecular biology methods have led to the creation new technologies that make it possible modify plant genomes by transferring integrating into genomes’ heterologous genes from various expression systems (genetic engineering), as well inducing knockouts one or more target interest (genomic editing). genome-editing is a milestone in modern breeding certainly relies on knowledge developed for transgenesis. This review will discuss issues related advantages disadvantages both improving economically valuable traits important crops.

Language: Английский

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Genome editing in food and agriculture: from regulations to consumer perspectives

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103127 - 103127

Published: April 2, 2024

Language: Английский

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Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 1, 2025

Language: Английский

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CRISPR–Cas applications in agriculture and plant research

Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

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Unconventional sources of vegetable proteins: technological properties

Current Opinion in Food Science, Journal Year: 2024, Volume and Issue: 57, P. 101150 - 101150

Published: March 6, 2024

Language: Английский

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Engineering plants using diverse CRISPR-associated proteins and deregulation of genome-edited crops

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 42(5), P. 560 - 574

Published: Nov. 21, 2023

Language: Английский

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The evolving landscape of global regulations on genome-edited crops

Journal of Plant Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 32(4), P. 831 - 845

Published: Nov. 23, 2023

Language: Английский

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Transforming plant‐based alternatives by harnessing precision fermentation for next‐generation ingredients

Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Global levels of critical food insecurity continue to rise as the projected population is expected reach approximately 10 billion in 2050. Meeting growing demands for protein, improved nutritional quality, and environmental sustainability requires transitioning from animal-sourced products. Plant-based products have emerged most prominent major alternative solution toward improving current production system; however, plant-based come with their flaws. Generating flavorful, nutritious, high-protein derived entirely plant sources typically lacks consumer acceptance. However, field precision fermentation within systems offers a substantial enhancing revolutionizing derive more textural analogs. Precision entails genetically modifying microorganisms such yeast, bacteria, microalgae, fungi, generate specific proteins, carbohydrates, lipids, vitamins, flavonoids. closing flavor gap between traditional animal-based This process provides controlled key ingredients that may improve final product's safety, sustainability, sensory value. Currently, well-known example fermented integrated into foods heme protein burgers, but there even greater potential. review highlights through microbial enhance organoleptic quality alternatives. © 2025 Society Chemical Industry.

Language: Английский

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Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 889 - 889

Published: March 12, 2025

Recent years have witnessed remarkable progress in plant biology, driven largely by the rapid evolution of CRISPR/Cas-based genome editing (GE) technologies. These tools, including versatile CRISPR/Cas systems and their derivatives, such as base editors prime editors, significantly enhanced universality, efficiency, convenience functional genomics, genetics, molecular breeding. However, traditional genetic transformation methods are essential for obtaining GE plants. depend on tissue culture procedures, which time-consuming, labor-intensive, genotype-dependent, challenging to regenerate. Here, we systematically outline current advancements simplifying GE, focusing optimization process through developmental regulators, development planta methods, establishment nanomaterial- viral vector-based delivery platforms. We also discuss critical challenges future directions achieving genotype-independent, culture-free GE.

Language: Английский

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