Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease DOI Creative Commons
Tim Kaden, Raquel Alonso‐Román, Johannes Stallhofer

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Inflammatory bowel disease (IBD) is an idiopathic gastrointestinal with drastically increasing incidence rates. Due to its multifactorial etiology, a precise investigation of the pathogenesis extremely difficult. Although reductionist cell culture models and more complex in animals have clarified understanding individual mechanisms contributing factors IBD past, it remains challenging bridge research clinical practice. Conventional 2D cannot replicate host–microbiota interactions stable long‐term microbial culture. Further, extrapolating data from animal patients due genetic environmental diversity leading differences immune responses. Human intestine organ‐on‐chip (OoC) emerged as alternative vitro model approach investigate IBD. OoC not only recapitulate human intestinal microenvironment accurately than cultures yet may also be advantageous for identification important disease‐driving pharmacological interventions targets possibility emulating different complexities. The predispositions biological hallmarks focusing on at mucosal barrier are elucidated here. Additionally, potential OoCs explore microbiota‐related therapies personalized medicine treatment discussed.

Language: Английский

Integrating machine learning and biosensors in microfluidic devices: A review DOI Creative Commons
Gianni Antonelli, Joanna Filippi, Michele D’Orazio

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116632 - 116632

Published: Aug. 3, 2024

Microfluidic devices are increasingly widespread in the literature, being applied to numerous exciting applications, from chemical research Point-of-Care devices, passing through drug development and clinical scenarios. Setting up these microenvironments, however, introduces necessity of locally controlling variables involved phenomena under investigation. For this reason, literature has deeply explored possibility introducing sensing elements investigate physical quantities biochemical concentration inside microfluidic devices. Biosensors, particularly, well known for their high accuracy, selectivity, responsiveness. However, signals could be challenging interpret must carefully analysed carry out correct information. In addition, proper data analysis been demonstrated even increase biosensors' mentioned qualities. To regard, machine learning algorithms undoubtedly among most suitable approaches undertake job, automatically highlighting biosensor signals' characteristics at best. Interestingly, it was also benefit themselves, a new paradigm that is starting name "intelligent microfluidics", ideally closing benefic interaction disciplines. This review aims demonstrate advantages triad microfluidics-biosensors-machine learning, which still little used but great perspective. After briefly describing single entities, different sections will benefits dual interactions, applications where reviewed employed.

Language: Английский

Citations

12
Developing organs‐on‐chips for biomedical applications DOI Creative Commons
Lingyu Sun, Hanxu Chen, Dongyu Xu

et al.

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(2)

Published: May 21, 2024

In recent years, organs-on-chips have been arousing great interest for their bionic and stable construction of crucial human organs in vitro. Compared with traditional animal models two-dimensional cell models, could not only overcome the limitations species difference poor predict ability but also be capable reappearing complex cell-cell interaction, tissue interface, biofluid other physiological conditions humans. Therefore, regarded as promising powerful tools diverse fields such biology, chemistry, medicine so on. this perspective, we present a review biomedical applications. After introducing key elements manufacturing craft organs-on-chips, intend to cut-edging applications fields, incorporating biological analysis, drug development, robotics Finally, emphasis is focused on perspectives organs-on-chips.

Language: Английский

Citations

10
Bioinspired Stimuli-Responsive Materials for Soft Actuators DOI Creative Commons
Zhongbao Wang, Yixin Chen, Yuan Ma

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(3), P. 128 - 128

Published: Feb. 21, 2024

Biological species can walk, swim, fly, jump, and climb with fast response speeds motion complexity. These remarkable functions are accomplished by means of soft actuation organisms, which commonly composed muscle tissue systems. To achieve the creation their biomimetic artificial counterparts, various stimuli-responsive materials have been synthesized developed in recent decades. They respond to external stimuli form structural or morphological transformations actively passively converting input energy into mechanical energy. core element actuators for typical smart devices like robots, muscles, intelligent sensors nanogenerators. Significant progress has made development bioinspired materials. However, these not comprehensively summarized specific mechanisms literature. In this review, we will discuss advances that instrumental actuators. Firstly, different principles discussed, including fluidic, electrical, thermal, magnetic, light, chemical stimuli. We further summarize state-of-the-art explore advantages disadvantages using electroactive polymers, magnetic composites, photo-thermal responsive shape memory alloys other Finally, provide a critical outlook on field emphasize challenges process implementation industries.

Language: Английский

Citations

8
Gut microbiota in health and disease: advances and future prospects DOI Creative Commons
Y J Zhang, Hong Wang, Yingpeng Sang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(12)

Published: Nov. 20, 2024

Abstract The gut microbiota plays a critical role in maintaining human health, influencing wide range of physiological processes, including immune regulation, metabolism, and neurological function. Recent studies have shown that imbalances composition can contribute to the onset progression various diseases, such as metabolic disorders (e.g., obesity diabetes) neurodegenerative conditions Alzheimer's Parkinson's). These are often accompanied by chronic inflammation dysregulated responses, which closely linked specific forms cell death, pyroptosis ferroptosis. Pathogenic bacteria trigger these death pathways through toxin release, while probiotics been found mitigate effects modulating responses. Despite insights, precise mechanisms influences diseases remain insufficiently understood. This review consolidates recent findings on impact immune‐mediated inflammation‐associated conditions. It also identifies gaps current research explores potential advanced technologies, organ‐on‐chip models microbiome–gut–organ axis, for deepening our understanding. Emerging tools, single‐bacterium omics spatial metabolomics, discussed their promise elucidating microbiota's disease development.

Language: Английский

Citations

8
Phage lysins for intestinal microbiome modulation: current challenges and enabling techniques DOI Creative Commons

Iris Pottie,

Roberto Vázquez, Tom Van de Wiele

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Aug. 6, 2024

The importance of the microbiota in intestinal tract for human health has been increasingly recognized. In this perspective, microbiome modulation, a targeted alteration microbial composition, gained interest. Phage lysins, peptidoglycan-degrading enzymes encoded by bacteriophages, are promising new class antibiotics currently under clinical development treating bacterial infections. Due to their high specificity, lysins considered microbiome-friendly. This review explores opportunities and challenges using as modulators. First, specificity endolysins, which can be further modulated protein engineering or delivery methods, is discussed. Next, obstacles possible solutions assess microbiome-friendliness considered. Finally, lysin discussed, including methods such particle-based probiotic vehicles. Mapping hurdles developing modulators identifying ways overcome these help development. way, application innovative antimicrobial agents expanded, thereby taking full advantage characteristics.

Language: Английский

Citations

5
Developing 3D bioprinting for organs-on-chips DOI
Zhuhao Wu, Rui Liu, Ning Shao

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Organs-on-chips (OoCs) can be directly fabricated by 3D bioprinting techniques, which enhance the structural and functional fidelity of organ models broaden applications OoCs.

Language: Английский

Citations

0
Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights DOI
Weifeng Hu, Yushen Wang,

Junlei Han

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review discusses advancements in organ-on-a-chip models, emphasizing gut, liver, and integrated gut–liver systems. It covers their applications disease modeling, toxicity testing, drug screening, as well future prospects.

Language: Английский

Citations

0
Host-microbe-cancer interactions on-a-chip DOI Creative Commons

Mauricio G. C. Sousa,

Danielle S.K. Brasino, Madeline Krieger

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 31, 2025

The tumor microbiota has emerged as a pivotal contributor to variety of cancers, impacting disease development, progression, and therapeutic resistance. Due the complexity microenvironment, reproducing interactions between microbes, cells, immune system remains great challenge for both in vitro vivo studies. To this end, significant progress been made toward leveraging tumor-on-a-chip model systems replicate critical hallmarks native vitro. These microfluidic platforms offer ability mimic essential components including controllable fluid flow conditions, manipulable extracellular matrix dynamics, intricate 3D multi-cellular communication. primary objective review is discuss recent challenges advances engineering host-microbiota on-a-chip. Ultimately, overcoming these obstacles will help us gain deeper insights into tumor-microbe enhance avenues developing more effective cancer therapies.

Language: Английский

Citations

0
Biomimetic culture substrates for modelling homeostatic intestinal epithelium in vitro DOI Creative Commons

Sorosh Abdollahi,

Bahareh Zarin,

Maryam Vatani

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 3, 2025

The increasing interest in utilizing three-dimensional (3D) vitro models with innovative biomaterials to engineer functional tissues arises from the limitations of conventional cell culture methods accurately reproducing complex physiological conditions living organisms. This study presents a strategy for replicating intricate microenvironment intestine by cultivating intestinal cells within bioinspired 3D interfaces that recapitulate villus-crypt architecture and tissue arrangement intestine. Intestinal cultured on these biomimetic substrates exhibited phenotypes differentiation characteristics resembling intestinal-specific types, effectively tissue. Notably, proliferation were achieved 72-120 h-significantly faster than several weeks required bioengineered materials, which often pose risks necrosis or cross-contamination. Additionally, differentiated villi-crypts mimicking bio-interfaces exhibit higher production natural antimicrobial peptides, resulting reduced pathogenic infection compared control samples. Furthermore, our method stands out simplicity fabrication, eliminating need cleanroom procedures microfabrication techniques.

Language: Английский

Citations

0
Bioengineered human gut-on-a-chip for advancing non-clinical pharmaco-toxicology DOI
Yong Cheol Shin, Nam Than,

Soo Jin Park

et al.

Expert Opinion on Drug Metabolism & Toxicology, Journal Year: 2024, Volume and Issue: 20(7), P. 593 - 606

Published: June 8, 2024

There is a growing need for alternative models to advance current non-clinical experimental because they often fail accurately predict drug responses in human clinical trials. Human organ-on-a-chip have emerged as promising approaches advancing the predictability of behaviors and responses.

Language: Английский

Citations

3