Integrative Analysis of miRNA Expression Profiles Reveals Distinct and Common Molecular Mechanisms Underlying Broad Diagnostic Groups of Severe Mental Disorders DOI Creative Commons
Jérémie Poschmann, Pierre Solomon, Lalit Kaurani

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 3, 2024

Abstract Micro RNAs (miRNAs) play a crucial role as regulators of various biological processes and have been implicated in the pathogenesis mental disorders such schizophrenia bipolar disorders. In this study, we investigate expression patterns miRNAs PsyCourse Study (n=1,786), contrasting three broad diagnostic groups: Psychotic (Schizophrenia-spectrum disorders), Affective (Bipolar Disorder I, II recurrent Depression), neurotypic healthy individuals. Through comprehensive analyses, including differential miRNA expression, transcriptome-wide association study (TWAS), predictive modelling, identified multiple unique to groups well shared by both. Furthermore, performed integrative analysis identify target genes dysregulated elucidate their potential roles psychosis. Our findings reveal significant alterations miR-584-3p miR-99b-5p across studied groups, highlighting molecular correlates. Additionally, TWAS discovered previously known novel genetically confirming relevance etiology groups. Importantly, factors putative mechanisms underlying these were uncovered through integration miRNA-target gene interactions. This investigation provides valuable insights into underpinnings severe disorders, shedding light on complex regulatory networks involving miRNAs.

Language: Английский

Identifying the differentially expressed peripheral blood microRNAs in psychiatric disorders: a systematic review and meta-analysis DOI Creative Commons
Xiaoyan Liu,

Liying Dong,

Zhaowei Jiang

et al.

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: May 17, 2024

Background Evidence has suggested that microRNAs (miRNAs) may play an important role in the pathogenesis of psychiatric disorders (PDs), but results remain inconclusive. We aimed to identify specific differentially expressed miRNAs and their overlapping miRNA expression profiles schizophrenia (SZ), major depression disorder (MDD), bipolar (BD), three PDs. Methods The literatures up September 30, 2023 related peripheral blood PDs were searched screened from multiple databases. differences levels between groups illustrated by standardized mean difference (SMD) 95% confidence interval (95% CI). Results In total, 30 included meta-analysis, including 16 for SZ, 12 MDD, 2 BD, each was reported more than 3 independent studies. Compared with control group, miR-181b-5p, miR-34a-5p, miR-195-5p, miR-30e-5p, miR-7-5p, miR-132-3p, miR-212-3p, miR-206, miR-92a-3p miR-137-3p upregulated while miR-134-5p, miR-107 miR-99b-5p downregulated. miR-124-3p, miR-139-5p, miR-182-5p, miR-221-3p, miR-34a-5p miR-93-5p upregulated, miR-144-5p miR-135a-5p However, we failed statistically BD. Interestingly, miR-132-3p both SZ MDD. Conclusions Our study identified 13 9 among which MDD systematically analyzing qualified These be used as potential biomarkers diagnosis future. Systematic Review Registration http://www.crd.york.ac.uk/PROSPERO , identifier CRD42023486982.

Language: Английский

Citations

5
Unraveling the potential of neuroinflammation and autophagy in schizophrenia DOI

Hongxia Tao,

Cheng‐Xiang Wang, Chuan Zou

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 997, P. 177469 - 177469

Published: March 5, 2025

Language: Английский

Citations

0
Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion DOI Creative Commons
Junyong Wu, Hai Huang, W. Xu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Severe acute pancreatitis (SAP) is a critical inflammatory disease with high morbidity and mortality. Current treatments focused on symptomatic relief but failed to prevent inflammation progression in cellular level. In order develop an SAP-targeting drug delivery system alleviate SAP level illustrate its mechanism, we explored the use of proanthocyanidin (PYD) pentoxifylline (PTX) loaded into macrophage membrane-coated self-assembly nanoparticles (FePTX@CM NPs) for targeted treatment. The combination application these two drugs was innovative aid. We developed NPs by strategy cell membrane coating. Its particle size zeta potential were measured dynamic light scatter (DLS). morphology observed transmission electron microscopy (TEM). And encapsulation efficiency evaluated nano-flow cytometry. total protein profile determined via Coomassie brilliant blue. explore mechanism our against animal levels. Bioinformatics approaches, TEM, immunofluorescent assay co-immunoprecipitation performed comprehensively explain specific anti-SAP FePTX@CM NPs. After inflammation-driven targeting, PYD inhibited pancreatic amylase lipase release suppressing mitochondrial reactive oxygen species (mtROS)/Golgi stress, while PTX prevented SAP-associated PANoptosis inhibiting Zbp1 signal pathway. protection effect biomimetic worked from different aspects symptoms relative cells. demonstrated effective pancreas reduced systemic especially pro-inflammatory recruitment activation, minimized tissue damage mouse models, offering promising therapeutic clinical management.

Language: Английский

Citations

0
A role for astrocytic miR-129-5p in frontotemporal dementia DOI Creative Commons
Lalit Kaurani,

Ranjit Pradhan,

Sophie Schröder

et al.

Translational Psychiatry, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 11, 2025

Abstract Frontotemporal dementia is a debilitating neurodegenerative disorder characterized by frontal and temporal lobe degeneration, resulting in behavioral changes, language difficulties, cognitive decline. In this study, smallRNA sequencing was conducted on postmortem brain tissues obtained from the of FTD patients with GRN , MAPT or C9ORF72 mutations. Our analysis identified miR-129-5p as consistently deregulated across all analyzed mutation conditions regions. Functional investigations in-vitro models revealed novel role astrocytes, where its loss led to neuroinflammation impaired neuronal support functions, including reduced glutamate uptake. Depletion astrocytes also resulted spines altered network activity cell culture system. These findings highlight potential therapeutic target diseases sheds light pathogenesis.

Language: Английский

Citations

0
Integrative analysis of miRNA expression profiles reveals distinct and common molecular mechanisms underlying broad diagnostic groups of severe mental disorders DOI
Pierre Solomon, Lalit Kaurani, Monika Budde

et al.

Molecular Psychiatry, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0
A role for astrocytic miR-129-5p in Frontotemporal Dementia DOI Creative Commons
Lalit Kaurani,

Ranjit Pradhan,

Sophie Schröder

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 19, 2024

Abstract Frontotemporal dementia is a debilitating neurodegenerative disorder characterized by frontal and temporal lobe degeneration, resulting in behavioral changes, language difficulties, cognitive decline. In this study, smallRNA sequencing was conducted on postmortem brain tissues obtained from FTD patients with GRN , MAPT or C9ORF72 mutations, focusing the lobes. Our analysis identified miR-129-5p as consistently deregulated across all mutation conditions regions. Functional investigations revealed novel role of astrocytes, where its loss led to neuroinflammation impaired neuronal support functions, including reduced glutamate uptake. Depletion astrocytes resulted spines altered network activity. These findings highlight potential therapeutic target diseases also sheds light pathogenesis.

Language: Английский

Citations

2
Integrative Analysis of miRNA Expression Profiles Reveals Distinct and Common Molecular Mechanisms Underlying Broad Diagnostic Groups of Severe Mental Disorders DOI Creative Commons
Jérémie Poschmann, Pierre Solomon, Lalit Kaurani

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 3, 2024

Abstract Micro RNAs (miRNAs) play a crucial role as regulators of various biological processes and have been implicated in the pathogenesis mental disorders such schizophrenia bipolar disorders. In this study, we investigate expression patterns miRNAs PsyCourse Study (n=1,786), contrasting three broad diagnostic groups: Psychotic (Schizophrenia-spectrum disorders), Affective (Bipolar Disorder I, II recurrent Depression), neurotypic healthy individuals. Through comprehensive analyses, including differential miRNA expression, transcriptome-wide association study (TWAS), predictive modelling, identified multiple unique to groups well shared by both. Furthermore, performed integrative analysis identify target genes dysregulated elucidate their potential roles psychosis. Our findings reveal significant alterations miR-584-3p miR-99b-5p across studied groups, highlighting molecular correlates. Additionally, TWAS discovered previously known novel genetically confirming relevance etiology groups. Importantly, factors putative mechanisms underlying these were uncovered through integration miRNA-target gene interactions. This investigation provides valuable insights into underpinnings severe disorders, shedding light on complex regulatory networks involving miRNAs.

Language: Английский

Citations

0