Adducins regulate morphology and fate of neural progenitors during neocortical neurogenesis DOI Creative Commons

Chiara Ossola,

Nikola Cokorac,

Stefania Faletti

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Summary The evolutionary expansion of the mammalian neocortex is mediated by an increase in proliferative capacity neural progenitor cells. However, molecular machinery controlling proliferation apical and basal progenitors during neocortical development still poorly understood. Here we show that three actin-associated morpho-regulatory adducins (ADD1-3), underlie abundance developing mouse ferret vivo human cortical organoids. Over expression embryonic increases number protrusions progenitors, leading to their neuronal output. Conversely, knock-out ADD1 organoids, which also leads down-regulation other adducins, results reduced aberrant neurogenesis. Hence, fate are key cellular features underlying progression

Language: Английский

Canonical and non-canonical PRC1 differentially contribute to regulation of neural stem cell fate DOI Creative Commons
Janine Hoffmann, Theresa M. Schütze, Annika Kolodziejczyk

et al.

Life Science Alliance, Journal Year: 2025, Volume and Issue: 8(4), P. e202403006 - e202403006

Published: Feb. 11, 2025

Neocortex development is characterized by sequential phases of neural progenitor cell (NPC) expansion, neurogenesis, and gliogenesis. Polycomb-mediated epigenetic mechanisms are known to play important roles in regulating the lineage potential NPCs during development. The composition Polycomb repressive complex 1 (PRC1) highly diverse mammals was hypothesized contribute context-specific regulation fate. Here, we have performed a side-by-side comparison role canonical PRC1.2/1.4 non-canonical PRC1.3/1.5, all which expressed developing neocortex, NSC proliferation differentiation. We found that deletion Pcgf2/4 NSCs led strong reduction altered fate, both neurogenic gliogenic phase, whereas Pcgf3/5 played minor role. Mechanistically, genes encoding stem factors were bound PRC1 differentially upon deletion. Thus, rather than different subcomplexes contributing development, more significant proliferative, neurogenic, compared with PRC1.

Language: Английский

Citations

1
Indirect neurogenesis in space and time DOI
Stefan Thor

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(8), P. 519 - 534

Published: July 1, 2024

Language: Английский

Citations

4
Epigenetic and metabolic regulation of developmental timing in neocortex evolution DOI Creative Commons

Matilde Aquilino,

Nora Ditzer, Takashi Namba

et al.

Trends in Neurosciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

The human brain is characterized by impressive cognitive abilities. neocortex the seat of higher cognition, and expansion a hallmark evolution. While developmental programs are similar in different species, timing transitions capacity neural progenitor cells (NPCs) to proliferate differ, contributing increased production neurons during cortical development. Here, we review epigenetic regulation corticogenesis, focusing mostly on humans while building knowledge from studies mice. We discuss metabolic-epigenetic interplay as potential mechanism integrate extracellular signals into chromatin. Moreover, synthesize current understanding how metabolic deregulation can cause neurodevelopmental disorders. Finally, outline be investigated using organoid models.

Language: Английский

Citations

0
Epigenome Profiling Identifies H3K27me3 Regulation of Extra-Cellular Matrix Composition in Human Corticogenesis DOI
Nora Ditzer,

Ezgi Senoglu,

Theresa M. Schütze

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

2
Canonical and non-canonical PRC1 differentially contribute to the regulation of neural stem cell fate DOI Creative Commons
Janine Hoffmann, Theresa M. Schütze, Annika Kolodziejczyk

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 7, 2024

Abstract Neocortex development is characterized by sequential phases of neural progenitor cell (NPC) expansion, neurogenesis and gliogenesis. Polycomb-mediated epigenetic mechanisms are known to play important roles in regulating the lineage potential NPCs during development. The composition Polycomb Repressive Complex 1 (PRC1) highly diverse mammals was hypothesized contribute context-specific regulation fate. Here, we have performed side-by-side comparison role canonical PRC1.2/1.4 non-canonical PRC1.3/1.5, all which expressed developing neocortex, NSC proliferation differentiation. We found that deletion Pcgf2/4 NSCs led a strong reduction altered fate, both neurogenic gliogenic phase, whereas Pcgf3/5 played minor role. Mechanistically, genes encoding stem factors were bound PRC1 differentially upon deletion. Thus, rather than different sub-complexes contributing development, more significant proliferative, compared PRC1.

Language: Английский

Citations

1
Epi-regulate my brain: unlocking mechanisms of brain growth evolution DOI Creative Commons
Virginia Fernández, Vı́ctor Borrell

The EMBO Journal, Journal Year: 2024, Volume and Issue: 43(8), P. 1385 - 1387

Published: March 25, 2024

Humans and other great apes stand out among mammals for the big size advanced folding of their brains' cerebral cortex. This cortex expansion during evolution has been linked to acquisition higher cognitive abilities results from gene regulatory changes fetal development. New research in The EMBO Journal implicates growth factor EPIREGULIN epigenetic regulation its expression evolutionary

Language: Английский

Citations

0
Adducins regulate morphology and fate of neural progenitors during neocortical neurogenesis DOI Creative Commons

Chiara Ossola,

Nikola Cokorac,

Stefania Faletti

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Summary The evolutionary expansion of the mammalian neocortex is mediated by an increase in proliferative capacity neural progenitor cells. However, molecular machinery controlling proliferation apical and basal progenitors during neocortical development still poorly understood. Here we show that three actin-associated morpho-regulatory adducins (ADD1-3), underlie abundance developing mouse ferret vivo human cortical organoids. Over expression embryonic increases number protrusions progenitors, leading to their neuronal output. Conversely, knock-out ADD1 organoids, which also leads down-regulation other adducins, results reduced aberrant neurogenesis. Hence, fate are key cellular features underlying progression

Language: Английский

Citations

0