bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2019,
Volume and Issue:
unknown
Published: June 7, 2019
Abstract
The
methylation
profile
of
circulating
cell-free
DNA
(cfDNA)
in
blood
can
be
exploited
to
detect
and
diagnose
cancer
other
tissue
pathologies
is
therefore
great
diagnostic
interest.
There
an
urgent
need
for
a
cost-effective
genome-wide
profiling
method
that
simple,
robust
automatable
works
on
highly
fragmented
cfDNA.
We
report
novel
sample
preparation
reduced
representation
bisulfite
sequencing
(RRBS),
rigorously
designed
customized
minute
amounts
DNA.
Our
particular
cfDNA
from
plasma.
It
performant
methodology
(termed
cf-RRBS)
which
enables
clinical
epigenomics
studies.
Pharmacy,
Journal Year:
2025,
Volume and Issue:
13(1), P. 7 - 7
Published: Jan. 22, 2025
The
complexities
surrounding
the
use
of
medications,
substance
abuse,
and
recreational
plants
are
multifaceted
warrant
a
comprehensive
examination.
This
review
highlights
consumption
chemical
substances
in
excess
or
for
non-medical
purposes,
obtained
through
legal
prescriptions,
over-the-counter
purchases,
illicit
means,
with
an
emphasis
on
predictive
role
stressors
individual-level
variables
development
disorders,
as
well
influence
regulatory
environment
patterns
consumption.
Additionally,
alarming
escalation
mortality
rate
associated
drug
opioid
overdoses
is
also
underscored.
prescription
medications
can
lead
to
significant
health
risks,
particularly
when
combined
other
substances;
therefore,
need
interventions
preventive
measures
address
abuse
among
various
populations
imperative.
Furthermore,
novel
insights
addiction,
exploring
neurobiological
mechanisms
underlying
discussing
treatment
approaches
elucidated.
Advancements
technology
detecting
highlighted,
displaying
innovative
tools
more
effective
identification
monitoring.
In
conclusion,
reveal
landscape
marked
by
overlapping
motivations
implications.
distinction
between
medical
critical
understanding
user
behavior
addressing
public
concerns.
Cambridge University Press eBooks,
Journal Year:
2020,
Volume and Issue:
unknown
Published: Aug. 13, 2020
This
multidisciplinary
volume
features
many
of
the
world's
leading
experts
infant
development,
who
synthesize
their
research
on
learning
and
behaviour,
while
integrating
perspectives
across
neuroscience,
socio-cultural
context,
policy.
It
offers
an
unparalleled
overview
development
foundational
areas
such
as
prenatal
brain
epigenetics,
physical
growth,
nutrition,
cognition,
language,
attachment,
risk.
The
chapters
present
theoretical
empirical
depth
rigor
specific
domains
highlighting
reciprocal
connections
among
brain,
behavior,
social-cultural
context.
handbook
simultaneously
educates,
enriches,
encourages.
educates
through
detailed
reviews
innovative
methods
foundations
enriches
by
considering
contexts
culture,
cutting-edge
establishes
agenda
for
future
policy,
highlights
findings
application
advanced
students,
researchers,
practitioners,
policy-makers
with
interests
in
understanding
promoting
development.
American Journal of Medical Genetics Part B Neuropsychiatric Genetics,
Journal Year:
2020,
Volume and Issue:
186(3), P. 173 - 182
Published: Aug. 17, 2020
Abstract
Cannabis
use
is
highly
prevalent
and
associated
with
adverse
beneficial
effects.
To
better
understand
the
full
spectrum
of
health
consequences,
biomarkers
that
accurately
classify
cannabis
are
needed.
DNA
methylation
(DNAm)
an
excellent
candidate,
yet
no
blood‐based
epigenome‐wide
association
studies
(EWAS)
in
humans
exist.
We
conducted
EWAS
lifetime
(ever
vs.
never)
using
DNAm
data
from
a
case–cohort
study
within
Sister
Study,
prospective
cohort
women
at
risk
developing
breast
cancer
(Discovery
N
=
1,730
[855
ever
users];
Replication
853
[392
users]).
identified
replicated
cg15973234
(CEMIP):
combined
p
3.3
×
10
−8
.
found
overlap
between
published
cis‐meQTLs
reported
use‐associated
single
nucleotide
polymorphism
(SNPs;
<
.05),
suggesting
observed
difference
was
driven
by
exposure.
also
developed
multi‐CpG
classifier
penalized
regression
top
CpGs.
The
resulting
50‐CpG
produced
area
under
curve
(AUC)
0.74
(95%
CI
[0.72,
0.76],
2.00
−5
)
discovery
sample
AUC
0.54
([0.51,
0.57],
2.87
−2
replication
sample.
Our
findings
provide
evidence
use.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2019,
Volume and Issue:
unknown
Published: June 7, 2019
Abstract
The
methylation
profile
of
circulating
cell-free
DNA
(cfDNA)
in
blood
can
be
exploited
to
detect
and
diagnose
cancer
other
tissue
pathologies
is
therefore
great
diagnostic
interest.
There
an
urgent
need
for
a
cost-effective
genome-wide
profiling
method
that
simple,
robust
automatable
works
on
highly
fragmented
cfDNA.
We
report
novel
sample
preparation
reduced
representation
bisulfite
sequencing
(RRBS),
rigorously
designed
customized
minute
amounts
DNA.
Our
particular
cfDNA
from
plasma.
It
performant
methodology
(termed
cf-RRBS)
which
enables
clinical
epigenomics
studies.
