Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(48), P. 22067 - 22074
Published: Nov. 23, 2022
Terpene
cyclases
(TCs),
extraordinary
enzymes
that
create
the
structural
diversity
seen
in
terpene
natural
products,
are
traditionally
divided
into
two
classes,
class
I
and
II.
Although
mechanistic
features
of
TCs
well-known,
corresponding
details
II
counterparts
have
not
been
fully
characterized.
Here,
we
report
genome
mining
discovery
characterization
sesquiterpene
(STCs)
from
Streptomyces.
These
drimenyl
diphosphate
synthases
(DMSs)
first
STCs
shown
to
possess
β,γ-didomain
architecture.
High-resolution
X-ray
crystal
structures
DMS
Streptomyces
showdoensis
(SsDMS)
complex
with
both
a
farnesyl
Mg2+
unveiled
an
induced-fit
mechanism,
unprecedented
binding
mode,
finally
solving
one
lingering
questions
TC
enzymology.
This
study
supports
continued
for
novel
bacterial
provides
new
insights
canonical
will
lead
advances
engineering
synthetic
biology.
International Journal of Molecular Sciences,
Journal Year:
2021,
Volume and Issue:
22(11), P. 5710 - 5710
Published: May 27, 2021
Biodiversity
is
adversely
affected
by
the
growing
levels
of
synthetic
chemicals
released
into
environment
due
to
agricultural
activities.
This
has
been
driving
force
for
embracing
sustainable
agriculture.
Plant
secondary
metabolites
offer
promising
alternatives
protecting
plants
against
microbes,
feeding
herbivores,
and
weeds.
Terpenes
are
largest
among
PSMs
have
extensively
studied
their
potential
as
antimicrobial,
insecticidal,
weed
control
agents.
They
also
attract
natural
enemies
pests
beneficial
insects,
such
pollinators
dispersers.
However,
most
these
research
findings
shelved
fail
pass
beyond
laboratory
greenhouse
stages.
review
provides
an
overview
terpenes,
types,
biosynthesis,
roles
in
microbial
pathogens,
insect
pests,
weeds
rekindle
debate
on
using
terpenes
development
environmentally
friendly
biopesticides
herbicides.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(6), P. 5990 - 5990
Published: March 22, 2023
Plants
are
sessile
organisms
that
face
environmental
threats
throughout
their
life
cycle,
but
increasing
global
warming
poses
an
even
more
existential
threat.
Despite
these
unfavorable
circumstances,
plants
try
to
adapt
by
developing
a
variety
of
strategies
coordinated
plant
hormones,
resulting
in
stress-specific
phenotype.
In
this
context,
ethylene
and
jasmonates
(JAs)
present
fascinating
case
synergism
antagonism.
Here,
Ethylene
Insensitive
3/Ethylene
Insensitive-Like
Protein1
(EIN3/EIL1)
Jasmonate-Zim
Domain
(JAZs)-MYC2
the
JAs
signaling
pathways,
respectively,
appear
act
as
nodes
connecting
multiple
networks
regulate
stress
responses,
including
secondary
metabolites.
Secondary
metabolites
multifunctional
organic
compounds
play
crucial
roles
acclimation
plants.
exhibit
high
plasticity
metabolism,
which
allows
them
generate
near-infinite
chemical
diversity
through
structural
modifications,
likely
have
selective
adaptive
advantage,
especially
climate
change
challenges.
contrast,
domestication
crop
has
resulted
or
loss
phytochemicals,
making
significantly
vulnerable
stresses
over
time.
For
reason,
there
is
need
advance
our
understanding
underlying
mechanisms
hormones
respond
abiotic
stress.
This
knowledge
may
help
improve
adaptability
resilience
changing
climatic
conditions
without
compromising
yield
productivity.
Our
aim
review
was
provide
detailed
overview
responses
mediated
impact
on
Natural Product Reports,
Journal Year:
2020,
Volume and Issue:
38(5), P. 905 - 980
Published: Nov. 10, 2020
We
highlight
the
current
state
of
bacterial
terpenome,
emphasizing
discoveries,
structures,
biosynthetic
pathways,
and
biological
activities
these
terpenoid
natural
products.
Natural Product Reports,
Journal Year:
2021,
Volume and Issue:
39(2), P. 249 - 272
Published: Oct. 9, 2021
Terpenoids
have
diverse
bioecological
roles
in
all
kingdoms
of
life.
Here
we
discuss
the
evolution
and
ecological
functions
microbial
terpenoids
their
possible
applications.
Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(11), P. 4739 - 4745
Published: March 8, 2022
We
report
enantioselective
one-carbon
ring
expansion
of
aziridines
to
make
azetidines
as
a
new-to-nature
activity
engineered
"carbene
transferase"
enzymes.
A
laboratory-evolved
variant
cytochrome
P450BM3,
P411-AzetS,
not
only
exerts
unparalleled
stereocontrol
(99:1
er)
over
[1,2]-Stevens
rearrangement
but
also
overrides
the
inherent
reactivity
aziridinium
ylides,
cheletropic
extrusion
olefins,
perform
rearrangement.
By
controlling
fate
highly
reactive
ylide
intermediates,
these
evolvable
biocatalysts
promote
transformation
which
cannot
currently
be
performed
using
other
catalyst
classes.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(32), P. 18094 - 18103
Published: Aug. 4, 2023
Chiral
oxygenated
aliphatic
moieties
are
recurrent
in
biological
and
pharmaceutically
relevant
molecules
constitute
one
of
the
most
versatile
types
functionalities
for
further
elaboration.
Herein
we
report
a
protocol
straightforward
general
access
to
chiral
γ-lactones
via
enantioselective
oxidation
strong
nonactivated
primary
secondary
C(sp3)–H
bonds
readily
available
carboxylic
acids.
The
key
enabling
aspect
is
use
robust
sterically
encumbered
manganese
catalysts
that
provide
outstanding
enantioselectivities
(up
>99.9%)
yields
96%)
employing
hydrogen
peroxide
as
oxidant.
resulting
immediate
interest
preparation
inter
alia
natural
products
recyclable
polymeric
materials.
Natural Product Reports,
Journal Year:
2023,
Volume and Issue:
41(3), P. 402 - 433
Published: Dec. 18, 2023
This
review
offers
an
overview
of
the
canonical
and
noncanonical
class
II
terpene
cyclases,
including
sesquiterpene,
diterpene,
triterpene,
meroterpenoid
cyclases.
It
delves
into
their
sequences,
structures,
mechanisms,
engineering
studies.
