International Journal of Nanomedicine,
Journal Year:
2023,
Volume and Issue:
Volume 18, P. 4121 - 4142
Published: July 1, 2023
Introduction:
Currently,
conventional
treatments
of
hepatocellular
carcinoma
(HCC)
are
not
selective
enough
for
tumor
tissue
and
lead
to
multidrug
resistance
drug
toxicity.
Although
sorafenib
(SOR)
is
the
standard
first-line
systemic
therapy
approved
clinical
treatment
HCC,
its
poor
aqueous
solubility
rapid
clearance
result
in
low
absorption
efficiency
severely
limit
use
local
treatment.
Methods:
Herein,
we
present
synthesis
biodegradable
polymeric
Poly
(D,
L-Lactide-co-glycolide)
(PLGA)
particles
loaded
with
SOR
(PS)
by
emulsion-solvent
evaporation
process.
The
carefully
characterized
focusing
on
particle
size,
surface
charge,
morphology,
loading
content,
encapsulation
efficiency,
vitro
stability,
release
behaviour
tested
HepG2
cells.
Additionally,
PLGA
have
been
coupled
side
emitting
optical
fibers
(
se
OF)
integrated
a
microfluidic
device
light-triggered
release.
Results:
PS
size
248
nm,
tunable
charge
uniform
spherical
shape
without
aggregation.
shows
89.7%
highest
(8.9%)
between
SOR-loaded
formulations.
Treating
cells
containing
at
7.5
μM
their
viability
dampened
40%,
30%
17%
after
48,
129
168
hours
incubation,
respectively.
Conclusion:
high
sustained
profile
cellular
uptake
corroborate
enhanced
cytotoxicity
effect
HepG2.
With
prospect
developing
biomedical
tools
control
spatial
temporal
drugs,
successfully
demonstrated
potentiality
OF
carriers.
Our
prototypical
system
paves
way
new
devices
integrating
microfluidics,
fibers,
advanced
carriers
capable
deliver
minimally
invasive
locoregional
cancer
treatments.
Graphical
Abstract:
Keywords:
poly(lactic-co-glycolic
acid),
sorafenib,
emulsion
solvent
technique,
carcinoma,
fiber,
microfluidics
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 5, 2025
Abstract
Itaconate
(IA)
is
an
endogenous
metabolite
and
a
potent
regulator
of
the
innate
immune
system.
Its
use
in
immunomodulatory
therapies
has
faced
limitations
due
to
inherent
challenges
achieving
controlled
delivery
requirements
for
high
extracellular
concentrations
achieve
internalization
highly
polar
small
molecule
its
intracellular
therapeutic
activity.
Microparticle
(MP)-based
strategies
are
promising
approach
metabolites
through
macrophage
phagocytosis
subsequent
polymer
degradation-based
delivery.
Toward
goal
IA,
degradable
polyester
polymer-(poly(itaconate-co-dodecanediol))
based
IA
microparticles
(IA-MPs)
were
generated
using
emulsion
method,
forming
micron-scale
(∼
1.5
µm)
microspheres.
IA-MPs
characterized
with
respect
their
material
properties
release
kinetics
inform
particle
fabrication.
Treatment
murine
bone
marrow-derived
macrophages
optimized
concentration
0.1
mg/million
cells
enabled
phagocytosis-mediated
low
levels
cytotoxicity.
Flow
cytometry
demonstrated
IA-MP-specific
regulation
IA-sensitive
inflammatory
targets.
Metabolic
analyses
that
IA-MP
inhibited
oxidative
metabolism
induced
glycolytic
reliance,
consistent
established
mechanism
IA-associated
inhibition
succinate
dehydrogenase.
This
development
IA-based
provides
basis
additional
innovative
metabolite-based
microparticle
drug
systems
treatment
disease.
Applied Sciences,
Journal Year:
2021,
Volume and Issue:
11(9), P. 4305 - 4305
Published: May 10, 2021
Alzheimer’s
disease
(AD)
is
a
chronic
neurodegenerative
disorder
that
accounts
for
about
60%
of
all
diagnosed
cases
dementia
worldwide.
Although
there
are
currently
several
drugs
marketed
its
treatment,
none
capable
slowing
down
or
stopping
the
progression
AD.
The
role
blood-brain
barrier
(BBB)
plays
key
in
design
successful
treatment
this
disease.
Nanosized
particles
have
been
proposed
as
suitable
drug
delivery
systems
to
overcome
BBB
with
purpose
increasing
bioavailability
brain.
Biodegradable
poly
(lactic-co-glycolic
acid)
nanoparticles
(PLGA-NPs)
particularly
regarded
promising
they
can
be
surface-tailored
functionalized
molecules
site-specific
targeting.
In
review,
thorough
discussion
most
recent
functionalization
strategies
based
on
PLGA-NPs
AD
and
their
mechanisms
action
provided,
together
description
pathogenesis
brain
European Polymer Journal,
Journal Year:
2022,
Volume and Issue:
173, P. 111307 - 111307
Published: May 25, 2022
The
design
of
polymeric
nanoparticles
(PNPs)
for
delivery
and
controlled
release
drugs
has
been
increasing
in
the
last
decades,
where
materials
such
as
biocompatible
degradable
polyesters
have
advantages.
However,
it
is
necessary
to
control
modulate
size
(NPs)
prevent
uptake
clearance
by
reticuloendothelial
system
achieve
selective
on
a
pharmaceutical
target.
In
this
mediation,
modulation
particle
(PS)
polydispersity
index
(PDI)
one
most
sought
parameters.
work,
study
external
parameters
power,
time,
cycles
sonication
preparation
with
polylactic
acid
(PLA)
simple
emulsion-solvent
evaporation
(SE-SE)
technique
using
ultrasound
was
carried
out.
A
Box-Behnken
method
used
improve
optimize
conditions
obtaining
nanoformulation,
which
allowed
response
surfaces
figure
out
effect
PS,
PDI,
ζ-Potential.
This
showed
that
main
influential
parameter
PDI
followed
time
sonication,
finally,
number
cycles.
statistical
model
prediction
variables
obtain
diameters
between
110
240
nm,
0.09–0.32,
ζ-Potential
remained
relatively
constant
each
experiment
an
average
value
−21.0
mV.
adequate
preparing
PNPs
are
20%
power
employed,
20
s
two
pause
10
pulse
when
PLA
136
kDa
used.
optimized
NPs
were
144.5
±
0.5
nm
0.1347
0.0064,
respectively.
Additionally,
can
develop
desired
PS
nanoformulations
excellent
homogeneity.
International Journal of Nanomedicine,
Journal Year:
2023,
Volume and Issue:
Volume 18, P. 4121 - 4142
Published: July 1, 2023
Introduction:
Currently,
conventional
treatments
of
hepatocellular
carcinoma
(HCC)
are
not
selective
enough
for
tumor
tissue
and
lead
to
multidrug
resistance
drug
toxicity.
Although
sorafenib
(SOR)
is
the
standard
first-line
systemic
therapy
approved
clinical
treatment
HCC,
its
poor
aqueous
solubility
rapid
clearance
result
in
low
absorption
efficiency
severely
limit
use
local
treatment.
Methods:
Herein,
we
present
synthesis
biodegradable
polymeric
Poly
(D,
L-Lactide-co-glycolide)
(PLGA)
particles
loaded
with
SOR
(PS)
by
emulsion-solvent
evaporation
process.
The
carefully
characterized
focusing
on
particle
size,
surface
charge,
morphology,
loading
content,
encapsulation
efficiency,
vitro
stability,
release
behaviour
tested
HepG2
cells.
Additionally,
PLGA
have
been
coupled
side
emitting
optical
fibers
(
se
OF)
integrated
a
microfluidic
device
light-triggered
release.
Results:
PS
size
248
nm,
tunable
charge
uniform
spherical
shape
without
aggregation.
shows
89.7%
highest
(8.9%)
between
SOR-loaded
formulations.
Treating
cells
containing
at
7.5
μM
their
viability
dampened
40%,
30%
17%
after
48,
129
168
hours
incubation,
respectively.
Conclusion:
high
sustained
profile
cellular
uptake
corroborate
enhanced
cytotoxicity
effect
HepG2.
With
prospect
developing
biomedical
tools
control
spatial
temporal
drugs,
successfully
demonstrated
potentiality
OF
carriers.
Our
prototypical
system
paves
way
new
devices
integrating
microfluidics,
fibers,
advanced
carriers
capable
deliver
minimally
invasive
locoregional
cancer
treatments.
Graphical
Abstract:
Keywords:
poly(lactic-co-glycolic
acid),
sorafenib,
emulsion
solvent
technique,
carcinoma,
fiber,
microfluidics
