Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond DOI
Michael D. Parkins,

Bonita E. Lee,

Nicole Acosta

et al.

Clinical Microbiology Reviews, Journal Year: 2023, Volume and Issue: 37(1)

Published: Dec. 14, 2023

SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.

Language: Английский

Wastewater SARS-CoV-2 monitoring as a community-level COVID-19 trend tracker and variants in Ohio, United States DOI Open Access

Yuehan Ai,

Angela Davis, Dan Jones

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 801, P. 149757 - 149757

Published: Aug. 19, 2021

Language: Английский

Citations

156
Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK DOI Open Access
Luke S. Hillary, Kata Farkas, Kathryn H. Maher

et al.

Water Research, Journal Year: 2021, Volume and Issue: 200, P. 117214 - 117214

Published: May 8, 2021

Language: Английский

Citations

150
Challenges in Measuring the Recovery of SARS-CoV-2 from Wastewater DOI Creative Commons
Rose S. Kantor, Kara L. Nelson, Hannah Greenwald

et al.

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(6), P. 3514 - 3519

Published: March 3, 2021

Wastewater-based epidemiology is an emerging tool for tracking the spread of SARS-CoV-2 through populations. However, many factors influence recovery and quantification from wastewater, complicating data interpretation. Specifically, these may differentially affect measured virus concentration, depending on laboratory methods used to perform test. Many laboratories add a proxy wastewater samples determine losses associated with concentration extraction viral RNA. While measuring important process control, in this piece, we describe caveats limitations interpretation including that it typically does not account during RNA extraction. We recommend reporting directly alongside efficiency, rather than attempting correct efficiency. Even though ability compare concentrations different sampling locations determined using limited, (uncorrected recovery) can be useful public health response.

Language: Английский

Citations

137
Wastewater monitoring outperforms case numbers as a tool to track COVID-19 incidence dynamics when test positivity rates are high DOI Creative Commons
Xavier Fernández-Cassi, Andreas Scheidegger, Carola Bänziger

et al.

Water Research, Journal Year: 2021, Volume and Issue: 200, P. 117252 - 117252

Published: May 17, 2021

Wastewater-based epidemiology (WBE) has been shown to coincide with, or anticipate, confirmed COVID-19 case numbers. During periods with high test positivity rates, however, numbers may be underreported, whereas wastewater does not suffer from this limitation. Here we investigated how the dynamics of new infections estimated based on monitoring cases compare true incidence dynamics. We focused first pandemic wave in Switzerland (February April, 2020), when ranged up 26%. SARS-CoV-2 RNA loads were determined 2–4 times per week three Swiss treatment plants (Lugano, Lausanne and Zurich). Wastewater data combined a shedding load distribution an infection-to-case confirmation delay distribution, respectively, estimate infection Finally, estimates compared reference by validated compartmental model. Incidence found better track timing shape peak cases. In contrast, confirmations provided subsequent decline infections. Under regime high-test WBE thus provides critical information that is complementary clinical monitor trajectory.

Language: Английский

Citations

134
Wastewater Surveillance for SARS-CoV-2 on College Campuses: Initial Efforts, Lessons Learned, and Research Needs DOI Open Access
Sasha Harris-Lovett, Kara L. Nelson, Paloma I. Beamer

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2021, Volume and Issue: 18(9), P. 4455 - 4455

Published: April 22, 2021

Wastewater surveillance for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging approach to help identify risk of a disease (COVID-19) outbreak. This tool can contribute public health at both community (wastewater treatment system) and institutional (e.g., colleges, prisons, nursing homes) scales. paper explores successes, challenges, lessons learned from initial wastewater efforts colleges university systems inform future research, development implementation. We present experiences 25 college in United States that monitored campus SARS-CoV-2 during fall 2020 academic period. describe broad range approaches, findings, resources, impacts these efforts. These institutions size, social political geographies, include private institutions. Our analysis suggests monitoring requires consideration local information needs, sewage infrastructure, resources sampling analysis, dynamics, approaches interpretation communication results, follow-up actions. Most reported learning process experimentation, evaluation, adaptation was key progress. ongoing collaboration among diverse stakeholders including decision-makers, researchers, faculty, facilities staff, students, members.

Language: Английский

Citations

129
SARS-CoV-2 Wastewater Surveillance for Public Health Action DOI Creative Commons

Jill S. McClary-Gutierrez,

Mia Mattioli,

Perrine Marcenac

et al.

Emerging infectious diseases, Journal Year: 2021, Volume and Issue: 27(9), P. 1 - 8

Published: Aug. 19, 2021

Abstract Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has garnered extensive public attention during the disease pandemic as a proposed complement to existing systems. Over past year, methods detection and quantification of SARS-CoV-2 viral RNA in untreated sewage have advanced, concentrations wastewater been shown correlate with trends reported cases. Despite promise surveillance, these measurements translate into useful health tools, bridging communication knowledge gaps between researchers responders is needed. We describe key uses, barriers, applicability supporting decisions actions, including establishing ethics consideration monitoring. Although assess community infections not new idea, might be initiating event make this emerging tool sustainable nationwide system, provided that barriers are addressed.

Language: Английский

Citations

116
Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak DOI Creative Commons
Élisabeth Mercier, Patrick M. D’Aoust, Ocean Thakali

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Sept. 22, 2022

Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance locate monitor influenza-like cases outbreaks mitigate hospitalizations deaths. Currently, integration of is the only reliable method for reporting types subtypes warn emergent strains. The utility wastewater (WWS) during COVID-19 as a less resource intensive replacement or complement has been predicated on analyzing viral fragments in wastewater. We show here that virus targets stable partitions favorably solids fraction. By quantifying, typing, subtyping municipal primary sludge community outbreak, we forecasted citywide flu outbreak with 17-day lead time provided population-level near real-time feasibility WWS at neighbourhood levels real using minimal resources infrastructure.

Language: Английский

Citations

110
Comparison of approaches to quantify SARS-CoV-2 in wastewater using RT-qPCR: Results and implications from a collaborative inter-laboratory study in Canada DOI Open Access
Alex H. S. Chik, Melissa B. Glier, Mark R. Servos

et al.

Journal of Environmental Sciences, Journal Year: 2021, Volume and Issue: 107, P. 218 - 229

Published: March 6, 2021

Language: Английский

Citations

107
Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater DOI Creative Commons
Warish Ahmed, Wendy Smith, Suzanne Metcalfe

et al.

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(11), P. 1871 - 1880

Published: Jan. 28, 2022

We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low-prevalence COVID-19 locations Queensland, Australia, using CDC N1 N2 assays. The assay limit (ALOD), inhibition rates, performance characteristics each assay, along with positivity rates RT-qPCR RT-dPCR platforms, were evaluated by seeding known concentrations exogenous wastewater. ALODs approximately 2-5 times lower than those RT-qPCR. During sample processing, endogenous (n = 96) 24) wastewater samples separated, was extracted from both eluates pellets (solids). platform demonstrated a rate significantly greater that assays eluate (N1, p 0.0029; N2, 0.0003) pellet 0.0015; 0.0067) samples. results also indicated analysis wastewater, including may further increase sensitivity RT-dPCR.

Language: Английский

Citations

98
A wastewater-based epidemic model for SARS-CoV-2 with application to three Canadian cities DOI Creative Commons
Shokoofeh Nourbakhsh,

Aamir Fazil,

Michael Li

et al.

Epidemics, Journal Year: 2022, Volume and Issue: 39, P. 100560 - 100560

Published: April 9, 2022

The COVID-19 pandemic has stimulated wastewater-based surveillance, allowing public health to track the epidemic by monitoring concentration of genetic fingerprints SARS-CoV-2 shed in wastewater infected individuals. Wastewater-based surveillance for is still its infancy. In particular, quantitative link between clinical cases observed through traditional and signals from viral concentrations developing hampers interpretation data actionable public-health decisions. We present a modelling framework that includes both transmission at population level fate RNA particles sewage system after faecal shedding persons population. Using our mechanistic representation combined clinical/wastewater system, we perform exploratory simulations quantify effect effectiveness, interventions vaccination on discordance signals. also apply model three Canadian cities provide wastewater-informed estimates actual prevalence, effective reproduction number incidence forecasts. find paired with this model, can complement supporting estimation key epidemiological metrics hence better triangulate state an using alternative source.

Language: Английский

Citations

92