Suitability of aircraft wastewater for pathogen detection and public health surveillance DOI Creative Commons
Davey L. Jones, Jennifer M. Rhymes, Matthew J. Wade

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 856, P. 159162 - 159162

Published: Oct. 3, 2022

International air travel is now widely recognised as one of the primary mechanisms responsible for transnational movement and global spread SARS-CoV-2. Monitoring viral load novel lineages within human-derived wastewater collected from aircraft at transport hubs has been proposed an effective way to monitor importation frequency pathogens. The success this approach, however, highly dependent on bathroom defecation habits passengers during their journey. In study UK adults (n = 2103), we quantified likelihood prior departure, upon arrival both short- long-haul flights. results were then used assess capturing signal infected individuals hubs. To obtain a representative cross-section population, survey was stratified by geographical region, gender, age, parenting status, social class. We found that individual's defecate short-haul flights (< 6 h in duration) low 13 % total), but higher 36 %; > duration). This behaviour pattern among males younger age groups. maximum departure 39 %). Based known SARS-CoV-2 faecal shedding rates (30–60 %) equal probability being (71 inbound flights) (29 %), estimate likely capture ca. 8–14 cases entering UK. Monte Carlo simulations predicted would be present 14 62 under current pandemic conditions. conclude alone insufficient effectively all transboundary entries faecal-borne pathogens can form part wider strategy public heath surveillance national borders.

Language: Английский

Wastewater surveillance to infer COVID-19 transmission: A systematic review DOI Creative Commons
Shimoni Shah, Sylvia Xiao Wei Gwee,

Jamie Qiao Xin Ng

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 804, P. 150060 - 150060

Published: Sept. 8, 2021

Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.

Language: Английский

Citations

179
Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes DOI Creative Commons
Matthew J. Wade, Anna Lo Jacomo, Elena Armenise

et al.

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 424, P. 127456 - 127456

Published: Oct. 9, 2021

The COVID-19 pandemic has put unprecedented pressure on public health resources around the world. From adversity, opportunities have arisen to measure state and dynamics of human disease at a scale not seen before. In United Kingdom, evidence that wastewater could be used monitor SARS-CoV-2 virus prompted development National surveillance programmes. pace this work proven unique in monitoring national level, demonstrating importance wastewater-based epidemiology (WBE) for protection. Beyond COVID-19, it can provide additional value informing range biological chemical markers health. A discussion measurement uncertainty associated with wastewater, focusing lessons-learned from UK programmes is presented, showing sources impacting quality interpretation data decision-making, are varied complex. While some factors remain poorly understood, we present approaches taken by manage mitigate more tractable uncertainty. This provides platform integrate management into WBE activities as part global One Health initiatives beyond pandemic.

Language: Английский

Citations

170
SARS-CoV-2 Wastewater Surveillance for Public Health Action DOI Creative Commons

Jill S. McClary-Gutierrez,

Mia Mattioli,

Perrine Marcenac

et al.

Emerging infectious diseases, Journal Year: 2021, Volume and Issue: 27(9), P. 1 - 8

Published: Aug. 19, 2021

Abstract Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has garnered extensive public attention during the disease pandemic as a proposed complement to existing systems. Over past year, methods detection and quantification of SARS-CoV-2 viral RNA in untreated sewage have advanced, concentrations wastewater been shown correlate with trends reported cases. Despite promise surveillance, these measurements translate into useful health tools, bridging communication knowledge gaps between researchers responders is needed. We describe key uses, barriers, applicability supporting decisions actions, including establishing ethics consideration monitoring. Although assess community infections not new idea, might be initiating event make this emerging tool sustainable nationwide system, provided that barriers are addressed.

Language: Английский

Citations

116
An analysis of 45 large-scale wastewater sites in England to estimate SARS-CoV-2 community prevalence DOI Creative Commons
Mario Morvan, Anna Lo Jacomo, Célia Souque

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: July 25, 2022

Accurate surveillance of the COVID-19 pandemic can be weakened by under-reporting cases, particularly due to asymptomatic or pre-symptomatic infections, resulting in bias. Quantification SARS-CoV-2 RNA wastewater used infer infection prevalence, but uncertainty sensitivity and considerable variability has meant that accurate measurement remains elusive. Here, we use data from 45 sewage sites England, covering 31% population, estimate prevalence within 1.1% estimates representative surveys (with 95% confidence). Using machine learning phenomenological models, show differences between sampled sites, flow rate, influence estimation require careful interpretation. We find signals appear 4-5 days earlier comparison clinical testing are coincident with suggesting a leading indicator for symptomatic viral infections. Surveillance viruses complements strengthens surveillance, significant implications public health.

Language: Английский

Citations

90
Comparison of virus concentration methods and RNA extraction methods for SARS-CoV-2 wastewater surveillance DOI

Xiawan Zheng,

Yu Deng, Xiaoqing Xu

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 824, P. 153687 - 153687

Published: Feb. 5, 2022

Language: Английский

Citations

85
Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review DOI Open Access
Xander Bertels,

Phaedra Demeyer,

Siel Van den Bogaert

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 820, P. 153290 - 153290

Published: Jan. 20, 2022

Language: Английский

Citations

83
Detection and quantification of SARS-CoV-2 RNA in wastewater influent in relation to reported COVID-19 incidence in Finland DOI Creative Commons
Ananda Tiwari, Anssi Lipponen, Anna-Maria Hokajärvi

et al.

Water Research, Journal Year: 2022, Volume and Issue: 215, P. 118220 - 118220

Published: Feb. 23, 2022

Wastewater-based surveillance is a cost-effective concept for monitoring COVID-19 pandemics at population level. Here, SARS-CoV-2 RNA was monitored from total of 693 wastewater (WW) influent samples 28 treatment plants (WWTP, N = 21-42 per WWTP) in Finland August 2020 to May 2021, covering WW ca. 3.3 million inhabitants (∼ 60% the Finnish population). Quantity fragments 24 h-composite determined by using ultrafiltration method followed nucleic acid extraction and CDC N2 RT-qPCR assay. signals each WWTP were compared over time numbers confirmed cases (14-day case incidence rate) sewer network area. Over 10-month period with an extensive number samples, detection rate 79% (including 6% uncertain results, i.e., amplified only one out four, two original ten-fold diluted replicates), while 24% all exhibited gene copy above quantification limit. The range varied 33% 10% results) Pietarsaari 100% Espoo. Only six positive SARS-COV-2 when reported preceding 14 days zero. Overall, 14-day 7.0, 18, 36 100 000 persons within area probability detect 50%, 75% 95%, respectively. required significantly more cases: 75%, 95% 110, 152, 223 cases, respectively, persons. Multiple linear regression relationship between quantified 15 WWTPs (overall R

Language: Английский

Citations

76
COVID-19 surveillance in wastewater: An epidemiological tool for the monitoring of SARS-CoV-2 DOI Creative Commons
Sajida Maryam, Ihtisham Ul Haq, Galal Yahya

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 5, 2023

The coronavirus disease 2019 (COVID-19) pandemic has prompted a lot of questions globally regarding the range information about virus’s possible routes transmission, diagnostics, and therapeutic tools. Worldwide studies have pointed out importance monitoring early surveillance techniques based on identification viral RNA in wastewater. These indicated presence severe acute respiratory syndrome 2 (SARS-CoV-2) human feces, which is shed via excreta including mucus, saliva, sputum. Subsequently, they get dumped into wastewater, their wastewater provides possibility using it as tool to help prevent eradicate virus. Its still done many regions worldwide serves an “warning signal”; however, limitations also been identified.

Language: Английский

Citations

43
A comparison of precipitation and filtration-based SARS-CoV-2 recovery methods and the influence of temperature, turbidity, and surfactant load in urban wastewater DOI Creative Commons
Jessica L. Kevill, Cameron Pellett, Kata Farkas

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 808, P. 151916 - 151916

Published: Nov. 24, 2021

Wastewater-based epidemiology (WBE) has become a complimentary surveillance tool during the SARS-CoV-2 pandemic. Viral concentration methods from wastewater are still being optimised and compared, whilst viral recovery under different characteristics storage temperatures remains poorly understood. Using urban samples, we tested three methods; polyethylene glycol precipitation (PEG), ammonium sulphate (AS), CP select™ InnovaPrep® (IP) ultrafiltration. We found no major difference in faecal indicator virus (crAssphage) samples (n = 46) using these methods, PEG slightly (albeit non-significantly), outperformed AS IP for detection, as higher genome copies per litre (gc/l) was recorded larger proportion of samples. Next generation sequencing 8 paired revealed non-significant differences quality data between IP, though better less variable. A controlled experiment assessed impact suspended solids (turbidity; 0-400 NTU), surfactant load (0-200 mg/l), temperature (5-20 °C) on methods. recoveries were >20% with <10% turbid additional 0-18% 0-5% IP. Turbidity sample combined had significant effect (p > 0.05), negative correlates < 0.001 p 0.05, respectively). In conclusion, our results show that choice methodology small crAssphage within this study. contrast, turbidity, temperature, did affect recovery, highlighting need careful consideration used when working

Language: Английский

Citations

67
Prevalence and circulation patterns of SARS-CoV-2 variants in European sewage mirror clinical data of 54 European cities DOI
Shelesh Agrawal, Laura Orschler, Selina Schubert

et al.

Water Research, Journal Year: 2022, Volume and Issue: 214, P. 118162 - 118162

Published: Feb. 5, 2022

Language: Английский

Citations

65