Fluorescent-based nanosensors for selective detection of a wide range of biological macromolecules: A comprehensive review

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 206, P. 115 - 147

Published: Feb. 26, 2022

Thanks to their unique attributes, such as good sensitivity, selectivity, high surface-to-volume ratio, and versatile optical electronic properties, fluorescent-based bioprobes have been used create highly sensitive nanobiosensors detect various biological chemical agents. These sensors are superior other analytical instrumentation techniques like gas chromatography, high-performance liquid capillary electrophoresis for being biodegradable, eco-friendly, more economical, operational, cost-effective. Moreover, several reports also highlighted application in the early detection of biomarkers associated with drug-induced organ damage liver, kidney, or lungs. In present work, we comprehensively overviewed electrochemical that employ nanomaterials (nanoparticles/colloids quantum dots, carbon nanoscaled metal-organic frameworks, etc.) a variety macromolecules based on fluorescent emission spectra. addition, most important mechanisms methods sense amino acids, protein, peptides, enzymes, carbohydrates, neurotransmitters, nucleic vitamins, ions, metals, electrolytes, blood gases, drugs (i.e., anti-inflammatory agents antibiotics), toxins, alkaloids, antioxidants, cancer biomarkers, urinary metabolites urea, uric acid, creatinine), pathogenic microorganisms were outlined compared terms selectivity sensitivity. Altogether, small dimensions capability these nanosensors sensitive, label-free, real-time sensing chemical, biological, pharmaceutical could be array-based screening in-vitro in-vivo diagnostics. Although nanoprobes widely applied determining macromolecules, unfortunately, they many challenges limitations. Efforts must made minimize limitations utilizing an emphasis commercial developments. We believe current review can foster wider incorporation nanomedicine will particular interest researchers working fluorescence technology, material chemistry, coordination polymers, related research areas.

Language: Английский

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Coumarin-based near-infrared fluorogenic probes: Recent advances, challenges and future perspectives

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 480, P. 215020 - 215020

Published: Jan. 25, 2023

Language: Английский

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Multi-Signal Fluorescent Probe for Simultaneous Differentiation and Imaging of Hg2+, Cys, Hcy, and GSH in Living Cells and Zebrafish

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137427 - 137427

Published: Jan. 1, 2025

Language: Английский

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Red and Near-Infrared Fluorescent Probe for Distinguishing Cysteine and Homocysteine through Single-Wavelength Excitation with Distinctly Dual Emissions

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(12), P. 5006 - 5013

Published: March 16, 2022

Small-molecule biothiols, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), participate in various pathological physiological processes. It is still a challenge to simultaneously distinguish Cys Hcy because of their similar structures reactivities, as well the interference from high intramolecular concentration GSH. Herein, novel fluorescent probe, CySI, based on cyanine thioester was developed differentiate through single-wavelength excitation two distinctly separated emission channels. The probe exhibited turn-on fluorescence response at both 625 nm (the red channel) 740 near-infrared but only showed no With aid built-in self-calibration single dual emissions, simultaneous discriminative determinations were realized CySI excellent selectivity toward with fast response. This further exploited visualize exogenous cells channels under one excitation. Moreover, it could efficiently target mitochondria applied monitor endogenous fluctuations independently channel.

Language: Английский

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Mucus-Inspired Dynamic Hydrogels: Synthesis and Future Perspectives

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(44), P. 20137 - 20152

Published: Sept. 8, 2022

Mucus hydrogels at biointerfaces are crucial for protecting against foreign pathogens and the biological functions of underlying cells. Since mucus can bind to host both viruses bacteria, establishing a synthetic model system that emulate properties native be synthesized large scale would revolutionize mucus-related research is essential understanding pathways many infectious diseases. The synthesis such biofunctional in laboratory highly challenging, owing their complex chemical compositions specific interactions occur throughout gel network. In this perspective, we discuss basic structures diverse physicochemical responsible unique hydrogels. We scrutinize different approaches preparing mucus-inspired hydrogels, with examples. also recent what it reveals about challenges must addressed opportunities considered achieve desirable de novo

Language: Английский

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Near-infrared colorimetric and ratiometric fluorescent probe for dual-channel imaging of mitochondria and cysteine in the oxidative stress model

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 475, P. 146397 - 146397

Published: Oct. 2, 2023

Language: Английский

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Selenium: From fluorescent probes to biomedical application

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 493, P. 215278 - 215278

Published: June 24, 2023

Language: Английский

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A near-infrared self-assembled micellar nanoprobe for highly effective detection of cysteine in vitro and in vivo

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 382, P. 133564 - 133564

Published: Feb. 23, 2023

Language: Английский

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Accurate Detection of Hcy in Human Serum and Two-Photon Visualization of Atherosclerosis Using a Highly Specific Fluorescent Probe

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(24), P. 9173 - 9181

Published: June 6, 2023

As an important metabolic intermediate of sulfur-containing amino acids in human body, homocysteine (Hcy) is regarded as independent risk factor for atherosclerotic cardiovascular disease. Therefore, real-time monitoring the fluctuation Hcy level great importance early diagnosis well treatment atherosclerosis. Herein, a new two-photon (TP) fluorescent probe (RH-2) was developed via hydrogen bond-assisted strategy, which had high specificity detecting over cysteine (Cys) and glutathione (GSH) solution, cells, tissue. Probe RH-2 applied to quantitative determination serum successfully. Moreover, fluorescence (TPF) imaging abnormal expression aortic vessels liver model mice were fulfilled by RH-2. can be served potential tool understand function atherosclerosis, supplying clinical promise atherosclerosis (AS).

Language: Английский

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An activated near-infrared fluorescent probe with large Stokes shift for discrimination of bio-thiols

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 414, P. 135994 - 135994

Published: May 18, 2024

Language: Английский

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