Inhibition of calcium imbalance protects hepatocytes from vanadium exposure-induced inflammation by mediating mitochondrial-associated endoplasmic reticulum membranes in ducks

Poultry Science, Journal Year: 2023, Volume and Issue: 102(12), P. 103013 - 103013

Published: Aug. 18, 2023

Vanadium (V) is an essential mineral element in animals, but excessive V can lead to many diseases, affecting the health of humans and animals. However, molecular crosstalk between mitochondria-associated endoplasmic reticulum membranes (MAMs) inflammation under exposure still at exploratory stage. This study was conducted determine MAMs ducks. In this study, duck hepatocytes were treated with NaVO3 (0 μM, 100 200 μM) 2-aminoethyl diphenyl borate (2-APB) (IP3R inhibitor) alone or combination for 24 h. The data showed that exposure-induced cell vacuolization, enlarged intercellular space, decreased density viability. Meanwhile, hydrogen peroxide (H2O2), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS) levels upregulated treatment. addition, could a marked reduction structure, destruction membrane structure overload intracellular Ca2+ mitochondrial Ca2+. Moreover, treatment resulted notable upregulation MAMs-relevant factors (IP3R, Mfn2, Grp75, MCU, VDAC1) downregulated IL-18, IL-1β, lactate dehydrogenase (LDH) supernatant. Additionally, it also significantly elevated inflammation-relevant (NLRP3, ASC, caspase-1, MAVS, TXNIP). inhibition IP3R expression attenuated V-induced variations above indicators. Collectively, our results revealed maintenance calcium homeostasis protect from injury via MAMs.

Language: Английский

---

AQP1 Deficiency Drives Phthalate-Induced Epithelial Barrier Disruption through Intestinal Inflammation

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(27), P. 15334 - 15344

Published: June 25, 2024

Di-2-ethylhexyl phthalate (DEHP) is frequently used as a plasticizer to enhance the plasticity and durability of agricultural products, which pose adverse effects human health environment. Aquaporin 1 (AQP1) main water transport channel protein involved in maintenance intestinal integrity. However, impact DEHP exposure on gut its potential mechanisms remain elusive. Here, we determined that induced compromised duodenum structure, was concomitant with mitochondrial structural injury epithelial cells. Importantly, caused inflammatory cell damage strong response accompanied by activating TLR4/MyD88/NF-κB signaling pathway. Mechanistically, directly inhibits expression AQP1 thus leads an response, ultimately disrupting integrity barrier function. Collectively, our findings uncover role phthalate-induced disorders, could be promising therapeutic approach for treating patients disorders or diseases.

Language: Английский

---

Mitochondrial DNA Stress-Mediated Health Risk to Dibutyl Phthalate Contamination on Zebrafish (Danio rerio) at Early Life Stage

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(18), P. 7731 - 7742

Published: April 25, 2024

Plastics contaminations are found globally and fit the exposure profile of planetary boundary threat. The plasticizer dibutyl phthalate (DBP) leaching has occurred poses a great threat to human health ecosystem for decades, its toxic mechanism needs further comprehensive elucidation. In this study, environmentally relevant levels DBP were used exposure, developmental process, oxidative stress, mitochondrial ultrastructure function, DNA (mtDNA) instability release, mtDNA-cyclic GMP-AMP synthase-stimulator interferon genes (cGAS-STING) signaling pathway with inflammatory responses measured in zebrafish at early life stage. Results showed that caused impairments heart rate, hatching body length, mortality embryo. Additionally, elevated stress damaged function induced damage mtDNA mutations replication, transcription, methylation. stressed leaked into cytosol activated cGAS-STING inflammation, which ameliorated by co-treatment reactive oxygen species (ROS) scavenger, inhibitors cGAS or STING. Furthermore, larval results suggest DBP-induced toxicity energy disorder inflammation involved defects impaired swimming capability. These enhance interpretation stress-mediated risk environmental contaminants contribute scrutiny toxicants.

Language: Английский

---

SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Phthalates exposure is a major public health concern due to the accumulation in environment and associated with levels of testosterone reduction, leading adverse pregnancy outcomes. However, relationship between phthalate-induced level decline ferroptosis remains poorly defined.

Language: Английский

---

TNF/TNFR1 Signaling Mediates DEHP-Induced Hepatocyte Pyroptosis via the GSDMD–mtROS Axis

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Di(2-ethylhexyl) phthalate (DEHP), which is widely used in agricultural plastics, accumulates humans and animals through the food chain over time, resulting liver toxicity. Recent studies have reported that pyroptosis mitochondrial damage are closely related to a variety of diseases, but specific mechanism still unclear. To address this issue, vitro vivo hepatotoxicity models were established. The results demonstrated exposure DEHP caused buildup MEHP livers, altered metabolite composition, pyroptosis-like changes hepatocytes. After treatment, REDOX homeostasis was unbalanced, reactive oxygen species (mtROS) overproduced. activates mediated by TNF/TNFR1 signaling upregulates perforating protein GSDMD-N destroy membrane Above all, study elucidates potential involvement signaling-mediated confirms regulation helpful maintaining normal function.

Language: Английский

---

Cadmium induced Fak -mediated anoikis activation in kidney via nuclear receptors (AHR/CAR/PXR)-mediated xenobiotic detoxification pathway

Journal of Inorganic Biochemistry, Journal Year: 2021, Volume and Issue: 227, P. 111682 - 111682

Published: Dec. 2, 2021

Language: Английский

---

The novel role of the aquaporin water channel in lycopene preventing DEHP-induced renal ionic homeostasis disturbance in mice

Ecotoxicology and Environmental Safety, Journal Year: 2021, Volume and Issue: 226, P. 112836 - 112836

Published: Oct. 1, 2021

Di-(2-ethylhexyl) phthalate (DEHP), an extensively used plasticizer, can cause environmental pollution and organ injury. Lycopene (LYC) is a natural carotene that has the potential to prevent chronic diseases. To reveal effect of DEHP and/or LYC on kidney, male mice were treated with (5 mg/kg) (500 mg/kg or 1000 by gavage for 28 days. The study indicated caused glomerular atrophy, tubular expansion, disappearance mitochondrial membrane, cristae rupture. exposure increase expression aquaporin (AQP) subunits activity Ca2+-Mg2+-ATPase decrease Na+-K+-ATPase, which results in ion disorder. However, relieve kidney injury regulating ATPase, ATPase subunits, AQP subunit expression. was target antagonizing disturbance DEHP-induced renal damage.

Language: Английский

---

Disrupted microbiota-barrier-immune interaction in phthalates-mediated barrier defect in the duodenum

Chemosphere, Journal Year: 2022, Volume and Issue: 308, P. 136275 - 136275

Published: Sept. 1, 2022

Language: Английский

---

Heme-oxygenase-1 as a target for phthalate-induced cardiomyocytes ferroptosis

Environmental Pollution, Journal Year: 2022, Volume and Issue: 317, P. 120717 - 120717

Published: Nov. 21, 2022

Language: Английский

---

Lycopene regulates the mitochondrial unfolded protein response to prevent DEHP-induced cardiac mitochondrial damage in mice

Food & Function, Journal Year: 2022, Volume and Issue: 13(8), P. 4527 - 4536

Published: Jan. 1, 2022

Lycopene (LYC), as a kind of carotene, has antioxidant effects. Di(2-ethylhexyl) phthalate (DEHP) was used to improve the flexibility plastics. However, potential role LYC in DEHP induced cardiac injury mice remains unclear. Therefore, aim this study investigate and mechanism injury. Male ICR were treated with (500 or 1000 mg per kg BW day) and/or (5 for 28 days. The results histopathology ultrastructure showed that relieved decrease mitochondrial volume density myocardial fibre disorder by DEHP. Subsequently, attenuated DEHP-induced damage, unfolded protein response (UPRmt) activation, nuclear factor erythroid 2-related 2 (Nrf2) mediated oxidative stress heat shock (HSR) activation regulates UPRmt prevent damage. Thus, provided new evidence target treatment preventing disease.

Language: Английский

---