An Overview of Crosslinked Enzyme Aggregates: Concept of Development and Trends of Applications DOI

Oumaima Maroua Bouguerra,

Roswanira Abdul Wahab, Fahrul Huyop

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: 196(9), P. 5711 - 5739

Published: Jan. 5, 2024

Language: Английский

Fundamentals of bio-electrochemical sensing DOI Creative Commons
Mahesh M. Shanbhag, G. Manasa, Ronald J. Mascarenhas

et al.

Chemical Engineering Journal Advances, Journal Year: 2023, Volume and Issue: 16, P. 100516 - 100516

Published: June 7, 2023

The utilization of bio-functionalities such as biorecognition or catalysis derived them their name biosensors. Bio-electrochemical sensing is a new discipline that combines the advantages biological detection and electrochemical transduction. sensors are devices use materials enzymes, antibodies, DNA, cells receptors to detect target analytes in variety samples. Electrodes convert interactions into electrical impulses, which can be studied using various techniques. have demonstrated significant promise for clinical diagnostics, environmental monitoring, food safety, biotechnology. Biosensors received numerous applications recent years because they fast, simple, inexpensive practical applications. In this article, we cover basic principles, design strategies, immobilization regeneration techniques, along with bio-electrochemical sensors. Finally, article discusses rationale developing biosensors context bio-receptors applied.

Language: Английский

Citations

121

Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits DOI Open Access
Jnanada Shrikant Joshi, S. V. Homburg, Andrea Ehrmann

et al.

Polymers, Journal Year: 2022, Volume and Issue: 14(6), P. 1267 - 1267

Published: March 21, 2022

Atomic force microscopy (AFM) is one of the microscopic techniques with highest lateral resolution. It can usually be applied in air or even liquids, enabling investigation a broader range samples than scanning electron (SEM), which mostly performed vacuum. Since it works by following sample surface based on between tip and sample, interactions have to taken into account, making AFM irregular complicated, but other hand allows measurements more physical parameters pure topography. This especially important for biopolymers hydrogels used tissue engineering biotechnological applications, where elastic properties, charges influence mammalian cell adhesion growth as well many effects. review gives an overview modes relevant investigations shows several examples recent focusing polysaccharides chitosan, alginate, carrageenan different hydrogels, depicting also spectrum materials are reported literature.

Language: Английский

Citations

74

Acetylcholinesterase biosensors for electrochemical detection of neurotoxic pesticides and acetylcholine neurotransmitter: A literature review DOI
Vahulabaranan Rajagopalan,

Swethaa Venkataraman,

Devi Sri Rajendran

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 227, P. 115724 - 115724

Published: March 21, 2023

Language: Английский

Citations

58

Cellulase immobilization to enhance enzymatic hydrolysis of lignocellulosic biomass: An all-inclusive review DOI
Chaozhong Xu,

Shanshan Tong,

Liqun Sun

et al.

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 321, P. 121319 - 121319

Published: Aug. 22, 2023

Language: Английский

Citations

56

Recent advances in the biocatalytic mitigation of emerging pollutants: A comprehensive review DOI
Bernard Chukwuemeka Ekeoma,

Leonard Nnamdi Ekeoma,

Mohammad Yusuf

et al.

Journal of Biotechnology, Journal Year: 2023, Volume and Issue: 369, P. 14 - 34

Published: May 10, 2023

Language: Английский

Citations

49

Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications DOI Creative Commons
Antônio Luthierre Gama Cavalcante, Dayana Nascimento Dari, Francisco Izaias da Silva Aires

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(25), P. 17946 - 17988

Published: Jan. 1, 2024

Enzymes are widely used in biofuels, food, and pharmaceuticals.

Language: Английский

Citations

41

A Review on Lipases: Sources, Assays, Immobilization Techniques on Nanomaterials and Applications DOI
Yosri A. Fahim, Ahmed M. El‐Khawaga, Reem M. Sallam

et al.

BioNanoScience, Journal Year: 2024, Volume and Issue: 14(2), P. 1780 - 1797

Published: Feb. 17, 2024

Language: Английский

Citations

22

Covalent immobilization: A review from an enzyme perspective DOI Creative Commons

Theja Prabhakar,

Jacopo Giaretta, Riccardo Zulli

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158054 - 158054

Published: Nov. 1, 2024

Language: Английский

Citations

20

Immobilization: the promising technique to protect and increase the efficiency of microorganisms to remove contaminants DOI
Aya A. Najim, Ahmed Y. Radeef,

Ibrahim al‐Doori

et al.

Journal of Chemical Technology & Biotechnology, Journal Year: 2024, Volume and Issue: 99(8), P. 1707 - 1733

Published: March 8, 2024

Abstract Biodegradation of pollutants is one the most economical methods for their removal and usually accompanied by no production toxic by‐products. In general, this approach favored over others because it offers reduced expenses potential complete mineralization. order to enhance viability longevity bioremediation agents within polluted areas, becomes necessary immobilize cells. Cell immobilization refers procedure confining intact cells specific areas a device or material, without compromising essential biological functions. A wide variety carriers approaches have been used restriction various Immobilization techniques, such as microencapsulation, opened up new possibilities in biotechnology facilitating development artificial organs, cell therapies drug delivery systems. Researchers found promising outcomes applications through microorganisms. This enhances stability, reusability catalytic efficiency, making valuable strategy biocatalysis, other biotechnological processes. Notably, use immobilized microorganisms has led significant improvements pollutants, with some studies achieving 100% efficiency. When comparing degradation between free same time period, results demonstrated that achieved efficiency >21% more than microbial consortia. The primary objective review give an overview key scientific aspects related using cells, particular focus on techniques entrap © 2024 Society Chemical Industry (SCI).

Language: Английский

Citations

19

Aspects of Electrochemical Biosensors Using Affinity Assays DOI Creative Commons

Thor Pedersen,

Leonid Gurevich, Nils E. Magnusson

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 166 - 166

Published: March 4, 2025

In recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification proteins, nucleic acids, small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across variety fields, including medicine, food safety, quality/environmental control. State-of-the-art biomarker detection methods predominantly rely on standard immunoassay techniques, requiring specialized laboratory equipment trained personnel. This impedes broad commercial implementation biosensors in, e.g., Point-of-Care (PoC) settings where ease operation, portability, cost-efficiency are prioritized. Small, robust electrochemical promising alternative for analyzing in complex samples within PoC environments. Therefore, creating designing optimized sensing surfaces, immobilization strategies, efficient signal generation improving biosensor systems, which turn can real-world impact. present paper, we reviewed common electrode types geometries used approaches, discussed advantages drawbacks different methods, presented labeling strategies enhancement.

Language: Английский

Citations

3