3D-bioprinted cancer-on-a-chip: level-up organotypic in vitro models

Trends in biotechnology, Journal Year: 2021, Volume and Issue: 40(4), P. 432 - 447

Published: Sept. 20, 2021

Language: Английский

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Blood–brain barrier (BBB)-on-a-chip: a promising breakthrough in brain disease research

Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(19), P. 3579 - 3602

Published: Jan. 1, 2022

The blood-brain barrier (BBB) represents a key challenge in developing brain-penetrating therapeutic molecules. BBB dysfunction is also associated with the onset and progression of various brain diseases. BBB-on-a-chip (μBBB), an organ-on-chip technology, has emerged as powerful vitro platform that closely mimics human microenvironments. While μBBB technology seen wide application study cancer, its utility other disease models ("μBBB+") less appreciated. Based on advances evolution for diseases over last decade, we propose concept "μBBB+" system summarize major promising applications pathological studies, personalized medical research, drug development, multi-organ-on-chip approaches. We believe such sophisticated highly tunable further advancement understanding

Language: Английский

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Engineering Organ-on-a-Chip to Accelerate Translational Research

Micromachines, Journal Year: 2022, Volume and Issue: 13(8), P. 1200 - 1200

Published: July 28, 2022

We guide the use of organ-on-chip technology in tissue engineering applications. Organ-on-chip is a form microengineered cell culture platform that elaborates in-vivo like organ or microenvironments. The consists microfluidic channels, chambers, and stimulus sources emulate microenvironment. These platforms are typically engraved into an oxygen-permeable transparent material. Fabrication these materials requires microfabrication strategies, including soft lithography, 3D printing, injection molding. Here we provide overview what platform, where it can be used, composed of, how fabricated, operated. In connection with this topic, also introduce recent applications, different organs modeled on microscale using technology.

Language: Английский

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PBPK Modeling on Organs-on-Chips: An Overview of Recent Advancements

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: April 14, 2022

Organ-on-a-chip (OoC) is a new and promising technology, which aims to improve the efficiency of drug development realize personalized medicine by simulating in vivo environment vitro . Physiologically based pharmacokinetic (PBPK) modeling believed have advantage better reflecting absorption, distribution, metabolism excretion process drugs than traditional compartmental or non-compartmental models. The combination PBPK organ-on-a-chip provide strong tool for potential replace animal testing. This article provides recent technology including model construction, parameter estimation validation strategies. Application on Organ-on-a-Chip has been emphasized, considerable progress made. OoC would become an essential part development, other fields.

Language: Английский

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Induced pluripotent stem cell-based organ-on-a-chip as personalized drug screening tools: A focus on neurodegenerative disorders

Journal of Tissue Engineering, Journal Year: 2022, Volume and Issue: 13

Published: Jan. 1, 2022

The Organ-on-a-Chip (OoC) technology shows great potential to revolutionize the drugs development pipeline by mimicking physiological environment and functions of human organs. translational value OoC is further enhanced when combined with patient-specific induced pluripotent stem cells (iPSCs) develop more realistic disease models, paving way for a new generation patient-on-a-chip devices. iPSCs differentiation capacity leads invaluable improvements in personalized medicine. Moreover, connection single-OoC into multi-OoC or body-on-a-chip allows investigate drug pharmacodynamic pharmacokinetics through study multi-organs cross-talks. need breakthrough thanks this particularly relevant within field neurodegenerative diseases, where number patients increasing successful rate discovery worryingly low. In review we discuss current iPSC-based as screening models their implication therapies disorders.

Language: Английский

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Liver-on-a-chip devices: the pros and cons of complexity

AJP Gastrointestinal and Liver Physiology, Journal Year: 2022, Volume and Issue: 323(3), P. G188 - G204

Published: July 12, 2022

Physiologically relevant and broadly applicable liver cell culture platforms are of great importance in both drug development disease modeling. Organ-on-a-chip systems offer a promising alternative to conventional, static two-dimensional (2-D) cultures, providing much-needed cues such as perfusion, shear stress, three-dimensional (3-D) cell-cell communication. However, devices cover broad range complexity manufacture implementation. In this review, we summarize the key features human that should be reflected physiologically liver-on-a-chip model. We also discuss different material properties producing recent current progress field, highlighting types at levels complexity.

Language: Английский

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Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(11), P. 2301 - 2301

Published: Oct. 26, 2022

There is an urgent need for predictive in vitro models to improve disease modeling and drug target identification validation, especially neurological disorders. Cerebral organoids, as alternative methods vivo studies, appear now powerful tools decipher complex biological processes thanks their ability recapitulate many features of the human brain. Combining these innovative with microfluidic technologies, referred brain organoids-on-chips, allows us model microenvironment several neuronal cell types 3D. Thus, this platform opens new avenues create a relevant approach preclinical applications neuroscience. The transfer pharmaceutical industry discovery stages adoption by scientific community requires proposition microphysiological systems allowing generation reproducible cerebral organoids high quality terms structural functional maturation, compatibility automation high-throughput screening. In review, we will focus on promising advantages how combination can enhance reproducibility models. Then, finish explaining why organoids-on-chips could be considered platforms pharmacological applications.

Language: Английский

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Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: April 27, 2023

Microfluidics is an interdisciplinary field that encompasses both science and engineering, which aims to design fabricate devices capable of manipulating extremely low volumes fluids on a microscale level. The central objective microfluidics provide high precision accuracy while using minimal reagents equipment. benefits this approach include greater control over experimental conditions, faster analysis, improved reproducibility. Microfluidic devices, also known as labs-on-a-chip (LOCs), have emerged potential instruments for optimizing operations decreasing costs in various industries, including pharmaceutical, medical, food, cosmetics. However, the price conventional prototypes LOCs generated clean room facilities, has increased demand inexpensive alternatives. Polymers, paper, hydrogels are some materials can be utilized create microfluidic covered article. In addition, we highlighted different manufacturing techniques, such soft lithography, laser plotting, 3D printing, suitable creating LOCs. selection fabrication techniques will depend specific requirements applications each individual LOC. This article comprehensive overview numerous alternatives development low-cost service industries pharmaceuticals, chemicals, biomedicine.

Language: Английский

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Advances of microfluidic lung chips for assessing atmospheric pollutants exposure

Environment International, Journal Year: 2023, Volume and Issue: 172, P. 107801 - 107801

Published: Feb. 1, 2023

Atmospheric pollutants, including particulate matters, nanoparticles, bioaerosols, and some chemicals, have posed serious threats to the environment human's health. The lungs are responsible organs for providing interface between circulatory system external environment, where pollutant particles can deposit or penetrate into bloodstream circulation. Conventional studies decipher mechanism underlying air pollution human health quite limited, due lack of reliable models that reproduce in vivo features lung tissues after pollutants exposure. In past decade, advanced near-to-native chips, combining cell biology with bioengineered technology, present a new strategy atmospheric assessment narrow gap 2D culture animal models. this review, key artificial chips cutting-edge technologies chip manufacture introduced. recent progresses exposure summarized highlighted. We further discuss current challenges future opportunities development their potential utilities associated toxicity testing drug screening.

Language: Английский

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Compound Absorption in Polymer Devices Impairs the Translatability of Preclinical Safety Assessments

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(11)

Published: Dec. 6, 2023

Organotypic and microphysiological systems (MPS) that can emulate the molecular phenotype function of human tissues, such as liver, are increasingly used in preclinical drug development. However, despite their improved predictivity, development success rates have remained low with most compounds failing clinical phases promising data. Here, it is tested whether absorption small molecules to polymers commonly for MPS fabrication impact pharmacological toxicological assessments contribute high failure rates. To this end, identical devices fabricated from eight different prototypic physicochemical properties analyzed. It found overall primarily driven by compound hydrophobicity number rotatable bonds. differ >1000-fold between polydimethyl siloxane (PDMS) being absorptive, whereas polytetrafluoroethylene (PTFE) thiol-ene epoxy (TEE) absorbed least. Strikingly, organotypic primary liver cultures successfully flagged hydrophobic hepatotoxins lowly absorbing TEE at therapeutically relevant concentrations, isogenic PDMS resistant, resulting false negative safety signals. Combined, these results guide selection materials facilitate assays translatability.

Language: Английский

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