A Serum Metabolomics Study Based on LC-MS: Chemosensitization Effects of Rauvolfia Vomitoria Afzel. Combined with 5-Fluorouracil on Colorectal Cancer Mice DOI
Zhifan Wang,

Wen-Ru Kong,

Ning Wang

et al.

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Colorectal cancer (CRC) is one of the malignant tumors with high incidence, and mainly treated by chemotherapy at present. However, during CRC treatment, long-term use traditional chemotherapeutic drugs will reduce sensitivity chemotherapy. Our previous studies have shown that Rauvolfia vomitoria total alkaloids (RVA) played an important role in 5-fluorouracil (5-FU)chemosensitization therapy, but its intervention mechanism has not been clarified completely metabolic level. Therefore, this study, LC-MS based metabolomics was employed to explore 5-FU chemosensitization induced combination RVA conventional 5-FUTo evaluate effects RVA, serum samples were collected from control group, treatment low-dose high-dose combined group respectively. Serum analyzed HPLC-Q-Exactive Orbitrap/MS, metabolites identified HMDB database. After multivariate statistical analysis identification, 33 differential between among which glycerophospholipid pathway pentose glucuronate interconversions mostly affected. These results suggest may sensitize drug exert anti-tumor activity through influencing glycerophosphplipid metabolism cell energy metabolism. Metabolomics provided a new insight into estimate therapeutic effect dissection potential mechanisms Chinese medicine treating colorectal cancer.

Language: Английский

Metabolic Profiling of Aromatic Compounds DOI Creative Commons
Alisa Pautova

Metabolites, Journal Year: 2024, Volume and Issue: 14(2), P. 107 - 107

Published: Feb. 5, 2024

Metabolic profiling is a powerful modern tool in searching for novel biomarkers and indicators of normal or pathological processes the body [...]

Language: Английский

Citations

1
Tadalafil increases the antitumor activity of 5-FU through inhibiting PRMT5-mediated glycolysis and cell proliferation in colorectal cancer DOI Creative Commons
Yao Shen, Pan Zhao,

Kewei Dong

et al.

Cancer & Metabolism, Journal Year: 2022, Volume and Issue: 10(1)

Published: Dec. 6, 2022

Protein arginine methyltransferase 5 (PRMT5) is upregulated in multiple tumors and plays a pivotal role cancer cell proliferation. However, the of PRMT5 colorectal remains poorly understood. We detected expression level glycolytic enzymes using online databases lines by immunohistochemical staining, quantitative real-time polymerase chain reaction (qRT-PCR), western blotting. And MTT colony formation assays were conducted to investigate Then, we evaluated ECAR OCR levels biological energy analyzer status cancer, transcriptional regulation was dual luciferase reporter assay ChIP assay. Finally, efficacy combined treatment tadalafil 5-FU verified. highly expressed tissues compared with their normal counterparts correlated poor prognosis CRC patients. demonstrated that knockdown or loss function attenuated viability cells, while overexpression promoted Mechanistically, enhanced glycolysis through transcriptionally activating LDHA expression. In addition, inhibitor, tadalafil, rendered cells sensitive antitumor agent vitro vivo. Our data indicates proliferation partially may be potential target for therapy.

Language: Английский

Citations

7
Abrupt Dietary Change and Gradual Dietary Transition Impact Diarrheal Symptoms, Fecal Fermentation Characteristics, Microbiota, and Metabolic Profile in Healthy Puppies DOI Creative Commons

Pinfeng Liao,

Kang Yang,

Hongcan Huang

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(8), P. 1300 - 1300

Published: April 11, 2023

Dietary changes are inevitable for pets, yet little is known about the impact of different dietary change methods on gastrointestinal response. The current comparative study evaluated effects diarrheal symptoms, fecal fermentation characteristics, microbiota, and metabolic profile healthy puppies. A total 13 beagle puppies were randomly divided into two groups; in abrupt (AC) group given 260 g a chicken- duck-based extruded diet (CD)daily one-week transition period, whereas gradual (GT) fed according to ratio salmon-based (SA) CD diets with difference 40 per day seven consecutive days. Serum samples collected D7, D0 D7. results indicated that GT reduced incidence diarrhea throughout trial period. had no influence serum inflammatory factors or SCFAs, but isovaleric acid was significantly after GT. Meanwhile, 16S rRNA sequencing showed microbiota changed changes. Compared bacterial AC, relative abundances beneficial bacteria (i.e., Turicibacter Faecalibacterium) feces increased Additionally, both AC caused amino metabolism, while also altered lipid metabolism. histamine spermine concentrations, decreased concentrations metabolites such as 5-hydroxyindoleacetic serotonin. Our findings most likely rate by modulating composition metabolism gut microbiota.

Language: Английский

Citations

3
The role of serum acylcarnitine profiling for the detection of multiple solid tumors in humans DOI Creative Commons

Longjunyu Wu,

Chunhua Ye,

Qingchun Yao

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 10(1), P. e23867 - e23867

Published: Dec. 19, 2023

Metabolic reprogramming is an essential hallmark of cancer. Several studies have reported the dysregulation acylcarnitine (ACar) metabolism in tumor cells, suggesting that changes blood ACar may be related to growth. Accordingly, this study aimed understand alteration serum profiles various solid tumors and explore potential differential ACars as diagnostic biomarkers. A series 69 relatively abundant were identified via untargeted analysis. Then, targeted metabolomics was used describe metabolic alterations between normal controls patients with six types tumors. The results suggested correlated their carbon chain length saturation. had highly similar profiles, indicating fatty acid oxidation (FAO) pathways. Moreover, receiver operating curve analysis showed 16 (C8-C14) high capability towards studied Specifically, area under 10:2 isomer2 12:2 greater than 0.95. In conclusion, marked decrease levels medium- long-chain (C8-C18) suggests they FAO-based pathways, which could afford a common target for cancer therapy. Additionally, biomarkers diagnosing

Language: Английский

Citations

3
Fano Enhancement of SERS for Rapid Early Diagnosis of Colorectal Cancer DOI Creative Commons
Tianxun Gong,

Zhenjiang Wei,

Libin Huang

et al.

Nanoscale Advances, Journal Year: 2024, Volume and Issue: 6(23), P. 5949 - 5959

Published: Jan. 1, 2024

Patients benefit greatly from early detection of colorectal cancer, but present diagnostic procedures have high costs, low sensitivity, and specificity. However, it is still difficult to develop a strategy that can effectively detect cancer using high-throughput blood analysis. Fano resonance-boosted SERS platform label-free serum creates an effective system at the point care. We obtained 220 high-quality spectral datasets 88 healthy volunteers 132 patients with cancer. The biomarker detected in was further evaluated 100 tissues adjacent normal intestinal collected West China Biobanks, Hospital, Sichuan University. results showed 97 out paired samples, biomarkers were successfully platform. This demonstrates resonance-based highly for diagnosing

Language: Английский

Citations

0
A metabolomic and proteomic study to elucidate the molecular mechanisms of immunotherapy resistance in patients with oesophageal squamous cell carcinoma DOI Open Access
Lijuan Gao, Yongshun Chen

Biomedical Reports, Journal Year: 2023, Volume and Issue: 18(5)

Published: April 6, 2023

Systemic chemotherapy, the standard first-line treatment option for patients with advanced oesophageal squamous cell carcinoma (OSCC), results in a median survival of ~1 year. Immune checkpoint inhibitors are breakthrough oncology option; however, most OSCC develop primary and acquired resistance to programmed death receptor-1 (PD-1) monoclonal antibody, severely affecting their prognosis. Therefore, there is an urgent need investigate molecular mechanism underlying treatment. The present study aimed explore PD-1 antibody. Plasma samples were collected from treated immunotherapy, who achieved pathological response/partial response (CR/PR) or stable disease/progressive disease (SD/PD) after fourth cycle. TM-widely targeted metabolomics, widely lipidomics, DIA proteomics assays performed. Differential metabolites screened based on fold change (FC) ≥1.5 ≤0.67 VIP ≥1; differential proteins FC >1.5 <0.67 P<0.05. identified annotated mapped using Kyoto Encyclopedia Genes Genomes (KEGG) pathway databases. Gene Ontology KEGG A correlation network diagram was drawn expressed (Pearson coefficient) r>0.80 Finally, 197 113 screened, respectively, CR/PR SD/PD groups. enrichment analysis revealed that all these enriched cholesterol metabolism NF-κB phospholipase D signalling pathways. first demonstrate inhibitor may be attributed activation. This finding suggests targeting pathways promising novel therapeutic approach which improve prognosis undergoing immunotherapy.

Language: Английский

Citations

1
The ratio of PKM1/PKM2 is the key factor affecting the glucose metabolism and biological function of colorectal cancer cells DOI Open Access
Liang Ma, Xue Zhang,

Yan Liu

et al.

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(7), P. 3522 - 3535

Published: July 1, 2024

Despite evidence suggesting a significant role of pyruvate kinase muscle isozyme (PKM) in cancer development, its particular function colorectal (CRC) remains unclear. This study aimed to elucidate the specific and mechanism PKM isoforms, PKM1 PKM2, progression CRC.

Language: Английский

Citations

0
Emerging role of non-coding RNAs in glucose metabolic reprogramming and chemoresistance in colorectal cancer DOI Creative Commons
Shushan Yan, Shufeng Wang, Xinyi Wang

et al.

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: Aug. 1, 2022

Metabolic reprogramming plays a critical role in colorectal cancer (CRC). It contributes to CRC by shaping metabolic phenotypes and causing uncontrolled proliferation of cells. Glucose is common carcinogenesis progression. Growing evidence has implicated the modifying effects non-coding RNAs (ncRNAs) glucose chemoresistance CRC. In this review, we have summarized currently published studies investigating ncRNAs alterations Elucidating interplay between provides insight into exploring novel biomarkers for diagnosis prognosis prediction

Language: Английский

Citations

2
Recent Progress in Mass Spectrometry-based Metabolomics for Colorectal Cancer DOI

Chulei Xiao,

Quan Chi, Xian Wang

et al.

Chemical Research in Chinese Universities, Journal Year: 2022, Volume and Issue: 38(4), P. 886 - 893

Published: May 23, 2022

Language: Английский

Citations

1
Discovery of Synergistic Drug Combinations for Colorectal Cancer Driven by Tumor Barcode Derived from Metabolomics “Big Data” DOI Creative Commons

Bo Lv,

Ruijie Xu,

Xinrui Xing

et al.

Metabolites, Journal Year: 2022, Volume and Issue: 12(6), P. 494 - 494

Published: May 30, 2022

The accumulation of cancer metabolomics data in the past decade provides exceptional opportunities for deeper investigations into metabolism. However, integrating a large amount heterogeneous to draw full picture metabolic reprogramming and discover oncometabolites certain cancers remains challenging. In this study, tumor barcode constructed based upon existing "big data" using Bayesian vote-counting method is proposed identify colorectal (CRC). Specifically, panel CRC was generated from 39 clinical studies with 3202 blood samples (1332 vs. 1870 controls) 990 tissue (495 495 controls). Next, an oncometabolite-protein network by combining its involved proteins/enzymes. effect anti-cancer drugs or drug combinations then mapped random walk restart process. Utilizing network, potential Irinotecan (CPT-11)-sensitizing agents treatment were discovered forest Xgboost. Finally, compound named MK-2206 highlighted synergy CPT-11 validated on two cell lines. To summarize, we demonstrate present study that data"-based barcodes subsequent analyses are potentially useful combination discovery repositioning.

Language: Английский

Citations

1