Dual-channel structured illumination super-resolution quantitative fluorescence resonance energy transfer imaging DOI Open Access
Zewei Luo, Ge Wu, Zhi Chen

et al.

Acta Physica Sinica, Journal Year: 2023, Volume and Issue: 72(20), P. 208701 - 208701

Published: Jan. 1, 2023

The Structured illumination (SI)-based super resolution fluorescence resonance energy transfer (SR-FRET) imaging technique, known as SISR-FRET, enables the investigation of molecular structures and functions in cellular organelles by resolving sub-diffraction FRET signals within living cells. microscopy offers unique advantages for quantitatively detecting dynamic interactions spatial distribution biomolecules conventional is limited diffraction limit, it can only capture average behavior these events limits microscopy. SISR-FRET performs sequential linear reconstruction three-channel SIM images followed quantitative analysis using a common localization mask-based filtering approach. This two-step process ensures fidelity reconstructed SR-FRET while effectively removing false-positive caused artifacts. However, slow speed resulting from switching excitation-emission channels its application fast scenarios. To address this issue, study proposes dual-channel structured super-resolution system method. By incorporating an registration module into pathway, channel multiplexing are achieved. Combining image algorithm with sub-pixel correction, technique enhances temporal 3.5 times preserving analysis. Experimental results obtained multi-color to perform cells expressing mitochondria outer membrane standard plasmids. These experiments validate improved quantification In summary, research presents novel It overcomes limitations realizing channels. proposed maintaining FRET. validation demonstrates increased accuracy advancement contributes organelles, providing valuable insights intricate mechanisms

Language: Английский

Fecal Microbiota Transplantation in Liver Cirrhosis DOI Creative Commons
Adrian Boicean, Victoria Bîrluțiu, Cristian Ichim

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(11), P. 2930 - 2930

Published: Oct. 30, 2023

The human gastrointestinal tract houses a diverse array of probiotic and pathogenic bacteria any alterations in this microbial composition can exert significant influence on an individual’s well-being. It is well-established that imbalances the gut microbiota play pivotal role development liver diseases. In light this, new adjuvant therapy for diseases could be regulating intestinal microbiota. Through fecal transplantation, patients whose microbiomes are compromised treated with stool from healthy donors attempt to restore normal microbiome alleviate their symptoms. A review cross-sectional studies case reports suggests transplants may offer effective treatment chronic Adding potential emerging therapy, recent research has indicated transplantation holds promise as therapeutic approach specifically cirrhosis. By introducing range beneficial microorganisms into gut, innovative aims address often observed cirrhotic patients. While further validation still required, these preliminary findings highlight impact novel targeted method managing We aimed summarize current state understanding regarding procedure, cirrhosis, well explain its clinical application future potential.

Language: Английский

Citations

29
Fluorescence Resonance Energy Transfer Measurements in Polymer Science: A Review DOI Creative Commons

Sara Valdez,

Mark Robertson, Zhe Qiang

et al.

Macromolecular Rapid Communications, Journal Year: 2022, Volume and Issue: 43(24)

Published: June 11, 2022

Fluorescence resonance energy transfer (FRET) is a non-invasive characterization method for studying molecular structures and dynamics, providing high spatial resolution at nanometer scale. Over the past decades, FRET-based measurements are developed widely implemented in synthetic polymer systems understanding detecting variety of nanoscale phenomena, enabling significant advances science. In this review, basic principles fluorescence FRET briefly discussed. Several representative research areas highlighted, where spectroscopy imaging can be employed to reveal morphology kinetics. These examples include micelle formation stability, guest molecule release from host, characterizing supramolecular assembly, composite interfaces, determining chain conformations their diffusion Finally, perspective on opportunities provided further allowing greater contributions exciting area.

Language: Английский

Citations

28
Upconversion luminescent sensor for endogenous H2O2 detection in cells based on the inner filter effect of coated silver layer DOI
Zhuo Wang,

Yale Hong,

Jiabei Li

et al.

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 376, P. 132936 - 132936

Published: Nov. 1, 2022

Language: Английский

Citations

22
Principles of Resonance Energy Transfer DOI Creative Commons

Ágnes Szabó,

János Szöllősi, Péter Nagy

et al.

Current Protocols, Journal Year: 2022, Volume and Issue: 2(12)

Published: Dec. 1, 2022

Abstract This unit describes the basic principles of Förster resonance energy transfer (FRET). Beginning with a brief summary history FRET applications, theory is introduced in detail using figures to explain all important parameters process. After listing various approaches for measuring efficiency, several pieces advice are given on choosing appropriate instrumentation. The concludes discussion limitations measurements followed by few examples latest including new developments such as spectral flow cytometric FRET, single‐molecule and combinations super‐resolution or lifetime imaging microscopy molecular dynamics simulations. © 2022 Authors. Current Protocols published Wiley Periodicals LLC. article was corrected 20 December 2022. See end full text details.

Language: Английский

Citations

19
Nanoparticles for super-resolution microscopy: intracellular delivery and molecular targeting DOI Creative Commons
Sumit Kumar Pramanik,

Sreejesh Sreedharan,

Rajeshwari Tiwari

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(24), P. 9882 - 9916

Published: Jan. 1, 2022

Following an overview of the approaches and techniques used to acheive super-resolution microscopy, this review presents advantages supplied by nanoparticle based probes for these applications.

Language: Английский

Citations

18
Keratin: A potential driver of tumor metastasis DOI
Yu‐Chuan Li,

Yiming Sun,

Kun Yu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141752 - 141752

Published: March 1, 2025

Language: Английский

Citations

0
Super‐Resolved FRET Imaging by Confocal Fluorescence‐Lifetime Single‐Molecule Localization Microscopy DOI Creative Commons
Cecilia Zaza,

Germán Chiarelli,

Ludovit Zweifel

et al.

Small Methods, Journal Year: 2023, Volume and Issue: 7(7)

Published: May 2, 2023

Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging Lifetime Imaging Microscopy (FLIM) enable visualization spatial distribution molecular functional states. However, conventional FLIM provide average information over an ensemble molecules within a diffraction-limited volume, which limits information, accuracy, dynamic range observed signals. Here, approach to obtain super-resolved based on single-molecule localization microscopy using early prototype commercial time-resolved confocal microscope is demonstrated. DNA Points Accumulation in Nanoscale Topography with fluorogenic probes provides suitable combination background reduction binding kinetics compatible scanning speed usual microscopes. A single laser used excite donor, broad detection band employed retrieve both donor acceptor emission, events detected from lifetime information.

Language: Английский

Citations

9
On the pixel selection criterion for the calculation of the Pearson's correlation coefficient in fluorescence microscopy DOI Creative Commons
Sergio G. López, Sebastian Samwald, Sally Jones

et al.

Journal of Microscopy, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 13, 2024

Abstract Colocalisation microscopy analysis provides an intuitive and straightforward way of determining if two biomolecules occupy the same diffraction‐limited volume. A popular colocalisation coefficient, Pearson's correlation coefficient (PCC), can be calculated using different pixel selection criteria: PCC ALL includes all image pixels, OR only pixels exceeding intensity thresholds for either one detection channels, AND both channels. Our results show that depends on foreground to background ratio, producing values influenced by factors unrelated biomolecular association. focuses areas with highest intensities in which allows it detect low levels colocalisation, but makes inappropriate evaluating spatial cooccurrence between signals. produces signal proportionality sometimes overemphasise lack latter. Overall, excels at detecting a balanced quantification coincidence, risks misinterpretation yet avoids segmentation challenges. Awareness their distinct properties should inform appropriate application aim accurately representing underlying biology.

Language: Английский

Citations

3
A novel upconversion nanoprobe-based approach for microalgae concentration determination DOI
Hong Wang,

Limin Wei,

Fangyun Xin

et al.

New Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Polymer-free upconversion nanoprobes are adopted as reliable sensors for the quantitative determination of microalgae concentration based on inner filter effect.

Language: Английский

Citations

0
Advancing super-resolution microscopy with metal complexes: functional imaging agents for nanoscale visualization DOI

Nur Aininie Yusoh,

Martin R. Gill, Xiaohe Tian

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights recent advancements in the use of metal complexes as functional imaging agents for nanoscale via super-resolution microscopy.

Language: Английский

Citations

0