Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
Patients
with
epidermal
growth
factor
receptor
mutant
nonsmall
cell
lung
cancer
(NSCLC)
often
fail
to
treat
gefitinib
because
of
secondary
drug
resistance.
The
development
tumor
resistance
is
closely
related
variations
in
metabolism.
Single-cell
metabolomics
analysis
can
provide
unique
information
about
Herein,
we
constructed
a
platform
study
the
cells
based
on
single-cell
(sSRTC-scM).
A
gefitinib-resistant
NSCLC
line
(PC9GR)
was
by
increasing
dose
step
step.
metabolic
profiles
parental
PC9
and
PC9GR
different
levels
were
detected
intact
living-cell
electrolaunching
ionization
mass
spectrometry
at
level.
data
analyzed
statistical
methods
such
as
t-SNE,
variance,
volcano
plot,
heat
map,
pathway
analysis.
Using
this
platform,
found
that
fingerprints
evaluate
degrees.
continue
be
altered
increase
We
revealed
19
markers
variance
clarified
glycerophospholipid
changed
significantly.
In
addition,
levels,
heterogeneity
metabolism
became
greater
number
weak
gradually
decreased.
This
phenomenon
utilized
illustrate
degrees
cells.
provides
diagnostic
for
evaluating
tumors
gives
new
insight
into
overcoming
tumors.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(4), P. 2312 - 2320
Published: Jan. 18, 2023
Cell
cycle
is
a
significant
factor
toward
cellular
heterogeneity,
so
cell
discrimination
precise
measurement
on
the
top
of
single-cell
analysis.
Single-cell
analysis
based
organic
mass
spectrometry
has
received
great
attention
for
its
unique
ability
to
profile
metabolome,
but
influence
metabolome
heterogeneity
been
overlooked
until
now
due
lack
compatible
method.
Here,
we
report
robust
protocol
combination
three
small
molecular
indicators,
consisting
two
labels
(Hoechst
and
docetaxel)
one
endogenous
compound
[phosphocholine
(34:1)],
discriminate
single
cells
at
different
stages
in
real
time
by
cytometry.
More
than
6000
HeLa
were
acquired
an
improved
cytometry
system
build
differentiation
model.
The
model
successfully
discriminated
cells,
SCC7,
Hep
G2
G0/G1,
S,
G2/M
with
larger
85%
sensitivity
89%
specificity.
Along
discrimination,
obvious
amino
acids,
nucleotides,
energy
metabolic
intermediates,
phospholipids
was
observed
among
this
protocol,
further
demonstrating
necessity
research
potential
more
compounds
discrimination.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(23), P. 8990 - 8997
Published: June 1, 2023
Nitric
oxide
(NO)
is
a
highly
reactive
signaling
molecule
involved
in
diverse
biological
processes.
Simultaneous
profiling
of
NO
and
associated
metabolic
fingerprints
single
assay
allows
more
accurate
assessments
cell
states
offers
the
possibility
to
better
understand
its
exact
roles.
Herein,
multiplexing
LC–MS
workflow
was
established
for
simultaneous
detection
intracellular
various
metabolites
based
on
novel
"iridium
signature"
mass
spectrometric
probe
(Ir-MSP841).
This
Ir-MSP841
can
convert
liable
stable
permanently
charged
triazole
product
(Ir-TP852),
enabling
direct
MS
NO.
191/193Ir-signature
probe-based
approach
endowed
with
overwhelming
advantages
interference-free,
high
quantitative
accuracy,
great
sensitivity
(limit
down
0.14
nM).
It
also
reveals
good
linearity
over
wide
concentration
range
12.5–500
nM
has
been
successfully
employed
exploring
release
behaviors
three
representative
donors
cells.
Meanwhile,
results
reveal
that
varying
concentrations
distinct
effects
cellular
metabolites.
study
provides
robust,
sensitive,
versatile
method
numerous
run
expands
applications
biomedical
research.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(27), P. 10221 - 10230
Published: June 26, 2023
Spatial
metabolomic
analysis
of
individual
tumor
spheroids
can
help
investigate
metabolic
rearrangements
in
different
cellular
regions
a
spheroid.
In
this
work,
nanocapillary-based
electrospray
ionization
mass
spectroscopy
(ESI-MS)
method
is
established
that
could
realize
the
spatial
sampling
components
single
living
spheroid
and
subsequent
MS
for
study.
During
penetration
nanocapillary
into
sampling,
"wound
surface"
at
outer
layer
takes
only
0.1%
whole
area
maximally
maintains
activity
inside
analysis.
Using
ESI-MS
analysis,
activities
inner
(upper
lower)
layers
are
revealed,
giving
full
investigation
heterogeneity
one
first
time.
addition,
between
two-dimensional
(2D)-cultured
cells
show
obvious
differences,
which
suggests
more
frequent
cell-cell
cell-extracellular
environment
interactions
during
culture
This
observation
not
establishes
powerful
tool
situ
but
also
provides
molecular
information
to
elucidate
three-dimensional
(3D)-cultured
cell
model.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
Patients
with
epidermal
growth
factor
receptor
mutant
nonsmall
cell
lung
cancer
(NSCLC)
often
fail
to
treat
gefitinib
because
of
secondary
drug
resistance.
The
development
tumor
resistance
is
closely
related
variations
in
metabolism.
Single-cell
metabolomics
analysis
can
provide
unique
information
about
Herein,
we
constructed
a
platform
study
the
cells
based
on
single-cell
(sSRTC-scM).
A
gefitinib-resistant
NSCLC
line
(PC9GR)
was
by
increasing
dose
step
step.
metabolic
profiles
parental
PC9
and
PC9GR
different
levels
were
detected
intact
living-cell
electrolaunching
ionization
mass
spectrometry
at
level.
data
analyzed
statistical
methods
such
as
t-SNE,
variance,
volcano
plot,
heat
map,
pathway
analysis.
Using
this
platform,
found
that
fingerprints
evaluate
degrees.
continue
be
altered
increase
We
revealed
19
markers
variance
clarified
glycerophospholipid
changed
significantly.
In
addition,
levels,
heterogeneity
metabolism
became
greater
number
weak
gradually
decreased.
This
phenomenon
utilized
illustrate
degrees
cells.
provides
diagnostic
for
evaluating
tumors
gives
new
insight
into
overcoming
tumors.
