Bioresource Technology,
Journal Year:
2023,
Volume and Issue:
381, P. 129144 - 129144
Published: May 11, 2023
This
study
reports
a
biocatalytic
system
of
immobilized
laccase
and
3D
printed
open-structure
biopolymer
scaffoldings.
The
scaffoldings
were
computer-designed
using
polylactide
(PLA)
filament.
immobilization
onto
the
PLA
scaffolds
optimized
with
regard
to
pH,
enzyme
concentration,
time.
Laccase
resulted
in
small
reduction
reactivity
(in
terms
Michaelis
constant
maximum
reaction
rate)
but
led
significant
improvement
chemical
thermal
stability.
After
20
days
storage,
free
showed
80%
35%
retention
initial
enzymatic
activity,
respectively.
on
achieved
10%
removal
estrogens
from
real
wastewater
as
compared
reusability
potential.
Results
here
are
promising
also
highlight
need
for
further
improve
activity
reusability.
Biotechnology Advances,
Journal Year:
2023,
Volume and Issue:
68, P. 108215 - 108215
Published: July 18, 2023
The
increasing
worries
by
the
inadequate
use
of
energy
and
preservation
nature
are
promoting
an
interest
in
production
biolubricants.
After
discussing
necessity
producing
biolubricants,
this
review
focuses
on
these
interesting
molecules
through
lipases,
different
possibilities
(esterification
free
fatty
acids,
hydroesterification
or
transesterification
oils
fats,
biodiesel
with
more
adequate
alcohols,
estolides
production,
modification
acids).
utilization
discarded
substrates
has
special
due
to
double
positive
ecological
impact
(e.g.,
oil
distillated,
overused
oils).
Pros
cons
all
possibilities,
together
general
considerations
optimize
processes
will
be
outlined.
Some
overcome
some
problems
detected
compounds
also
discussed.
International Journal of Biological Macromolecules,
Journal Year:
2023,
Volume and Issue:
248, P. 125853 - 125853
Published: July 17, 2023
Lipase
B
from
Candida
antarctica
(CALB)
and
lipase
Thermomyces
lanuginosus
(TLL)
have
been
immobilized
on
octyl
agarose
at
low
loading
a
exceeding
the
maximum
support
capacity.
Then,
enzymes
treated
with
glutaraldehyde
inactivated
pH
7
in
Tris-HCl,
sodium
phosphate
HEPES,
giving
different
stabilities.
Stabilization
(depending
buffer)
of
highly
loaded
biocatalysts
was
found,
very
likely
as
consequence
detected
intermolecular
crosslinkings.
This
did
not
occur
for
lowly
biocatalysts.
Next,
were
chemically
aminated
then
glutaraldehyde.
In
case
TLL,
intramolecular
crosslinkings
(visible
by
apparent
reduction
protein
size)
increased
enzyme
stability
biocatalysts,
an
effect
that
further
due
to
Using
CALB,
less
intense,
stabilization
lower,
even
though
quite
intense
biocatalyst.
The
depended
inactivation
buffer.
interactions
between
inactivating
buffer
effects
chemical
modifications
suggest
modification
studies
must
be
performed
under
target
conditions.
