Unspecific peroxygenases: The pot of gold at the end of the oxyfunctionalization rainbow?

Current Opinion in Green and Sustainable Chemistry, Journal Year: 2023, Volume and Issue: 41, P. 100786 - 100786

Published: Feb. 16, 2023

Fungal unspecific peroxygenases (UPOs) are among the most promising biocatalysts in synthetic chemistry. The kaleidoscope of oxyfunctionalization reactions that industrial and environmental relevance offer UPOs opportunity to provide solutions many processes. However, there important shortcomings must first be overcome order convert into applied biocatalysts, which include their general poor levels heterologous functional expression, presence unwanted peroxidase activity overoxidation reactions, as well oxidative inactivation provoked by co-substrate, hydrogen peroxide. specific engineering these proteins, they participate, is being used tackle principal challenges, with a view generate portfolio highly active, selective stable variants. In this review, we will discuss some recent developments true focusing on different trends currently followed fascinating field research.

Language: Английский

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Advancing the Enzymatic Removal of Antibiotics with Unspecific Peroxygenase and Vanadium Chloroperoxidase

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 115795 - 115795

Published: Feb. 1, 2025

Language: Английский

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Process intensification in continuous flow biocatalysis by up and downstream processing strategies

Current Opinion in Biotechnology, Journal Year: 2022, Volume and Issue: 78, P. 102835 - 102835

Published: Nov. 2, 2022

In this review, we focus on the holistic continuous enzymatic production and put special emphasis process intensification by up- downstream processing in flow biocatalysis. After a brief introduction, provide an overview of current examples enzyme immobilization as upstream for Thereafter, unit operations strategies, namely (i) liquid-liquid extraction, (ii) adsorptive processing, (iii) crystallization precipitation. Eventually, present our perspectives future trends research field.

Language: Английский

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From characterization to biocatalytic application of two peroxygenases from Collariella virescens and Daldinia caldariorum

Comptes Rendus Chimie, Journal Year: 2025, Volume and Issue: 28(G1), P. 141 - 150

Published: Feb. 21, 2025

Language: Английский

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A 3D printable synthetic hydrogel as an immobilization matrix for continuous synthesis with fungal peroxygenases

Reaction Chemistry & Engineering, Journal Year: 2023, Volume and Issue: 8(5), P. 984 - 988

Published: Jan. 1, 2023

Development of a novel immobilization strategy using synthetic 3D printable hydrogels for flow biocatalysis.

Language: Английский

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At-line monitoring of hydrogen peroxide released from its photocatalytic and continuous synthesis

Reaction Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 9(4), P. 777 - 781

Published: Jan. 1, 2024

Photocatalytic synthesis of H 2 O in a continuous operation set-up using nitrogen-doped carbon nanodots (N-CNDs) and simultaneous determination generated .

Language: Английский

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Unspecific peroxygenase immobilization in 3D-printed microfluidics: Towards tailor-made screening platforms

Catalysis Science & Technology, Journal Year: 2024, Volume and Issue: 14(22), P. 6496 - 6502

Published: Jan. 1, 2024

In the context of empowering biocatalysis, an easy-to-develop, reproducible, and easy-to-scale biocatalytic system is demonstrated as a microfluidic screening platform.

Language: Английский

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Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase

ChemSusChem, Journal Year: 2023, Volume and Issue: 16(23)

Published: June 26, 2023

Unspecific peroxygenases have attracted interest in synthetic chemistry, especially for the oxidative activation of C-H bonds, as they only require hydrogen peroxide (H2 O2 ) instead a cofactor. Due to their instability even small amounts H2 , different strategies like enzyme immobilization or situ production been developed improve stability these enzymes. While most studied separately, combination photocatalysis with immobilized enzymes was recently reported. To show advantages and limiting factors photobiocatalytic reaction, comparison is made between free Adjustment critical parameters such (i) substrate concentration, (ii) illumination wavelength (iii) light intensity results significantly increased stabilities variant. Moreover, under optimized conditions turnover number 334,500 reached.

Language: Английский

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Process intensification using immobilized enzymes

Physical Sciences Reviews, Journal Year: 2023, Volume and Issue: 9(8), P. 2737 - 2755

Published: May 31, 2023

Abstract The application potential of enzymes is undoubtedly very high. However, despite the large number different and enzyme activities, industrial processes comparatively small. particular challenge often lies in transferring promising laboratory to an scale. Here, required performance parameters, such as stability or productivity, must be achieved. On one hand, this can achieved by improving enzymes. other key indicators only using technical systems sense process intensification. In enzymatic processes, immobilization means choice enable processes. aim article outline most important methods summarize immobilized Finally, are compared a case study with unspecific peroxygenase.

Language: Английский

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Biocatalysis in microfluidic systems: an experimental basis for data science

Reaction Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 9(8), P. 2028 - 2033

Published: Jan. 1, 2024

Accelerated development of new biocatalytic processes using data science from designed microfluidic experiments, aimed at scale-down exposure enzyme variants to conditions mimicking those found in industrial processes.

Language: Английский

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