Process intensification using immobilized enzymes DOI

Sera Bolat,

Raphael Greifenstein, Matthias Franzreb

et al.

Physical Sciences Reviews, Journal Year: 2023, Volume and Issue: 9(8), P. 2737 - 2755

Published: May 31, 2023

Abstract The application potential of enzymes is undoubtedly very high. However, despite the large number different and enzyme activities, industrial processes comparatively small. particular challenge often lies in transferring promising laboratory to an scale. Here, required performance parameters, such as stability or productivity, must be achieved. On one hand, this can achieved by improving enzymes. other key indicators only using technical systems sense process intensification. In enzymatic processes, immobilization means choice enable processes. aim article outline most important methods summarize immobilized Finally, are compared a case study with unspecific peroxygenase.

Language: Английский

3D-Printed Hydrogel for Diverse Applications: A Review DOI Creative Commons
Arpana Agrawal, Chaudhery Mustansar Hussain

Gels, Journal Year: 2023, Volume and Issue: 9(12), P. 960 - 960

Published: Dec. 7, 2023

Hydrogels have emerged as a versatile and promising class of materials in the field 3D printing, offering unique properties suitable for various applications. This review delves into intersection hydrogels exploring current research, technological advancements, future directions. It starts with an overview hydrogel basics, including composition properties, details used printing. The explores diverse printing methods hydrogels, discussing their advantages limitations. emphasizes integration 3D-printed biomedical engineering, showcasing its role tissue regenerative medicine, drug delivery. Beyond healthcare, it also examines applications food, cosmetics, electronics industries. Challenges like resolution limitations scalability are addressed. predicts trends material development, techniques, novel

Language: Английский

Citations

30
3D printed and stimulus responsive drug delivery systems based on synthetic polyelectrolyte hydrogels manufactured via digital light processing DOI Creative Commons
Sonja Vaupel, R. Vinh Mau, Selin Kara

et al.

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(28), P. 6547 - 6559

Published: Jan. 1, 2023

Combining the best of both worlds: properties synthetic hydrogels and possibility complex structures 3D printing, obtaining an adjustable, flexible, sustainable drug delivery system.

Language: Английский

Citations

15
3D printing for flow biocatalysis DOI Creative Commons
Elena Gkantzou, Marie Weinhart, Selin Kara

et al.

RSC Sustainability, Journal Year: 2023, Volume and Issue: 1(7), P. 1672 - 1685

Published: Jan. 1, 2023

3D printing has been elaborated in all stages of a continuous flow biocatalytic process, from the reactor to support material for biocatalyst confinement or peripheral accessories that can establish highly controlled process.

Language: Английский

Citations

13
Functionalized Chitosan and Alginate Composite Hydrogel-Immobilized Laccase with Sustainable Biocatalysts for the Effective Removal of Organic Pollutant Bisphenol A DOI Open Access
Hong Zhang, Xin Zhang, Lei Wang

et al.

Catalysts, Journal Year: 2024, Volume and Issue: 14(5), P. 304 - 304

Published: May 3, 2024

The immobilization of enzymes is an important strategy to improve their stability and reusability. Enzyme technology has broad application prospects in biotechnology, biochemistry, environmental remediation, other fields. In this study, composites chitosan (CS) sodium alginate (SA) with Cu2+ forming a double-network crosslinked structure hydrogels were prepared used for the laccase. Fourier infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy tests revealed that laccase molecules immobilized on composite hydrogel surface by covalent bonding method. Compared free laccase, pH, temperature, storage markedly improved. addition, could be easily separated from reaction system reused, it maintained 81.6% its initial viability after six cycles use. Bisphenol A (BPA) polluted water was efficiently degraded using factors affecting degradation efficiency analyzed. Under optimal conditions, BPA removal greater than 82%, addition small amount ABTS had significant effect degradation, rate up 99.1%. Experimental results indicated laccases enormous potential actual industrial applications.

Language: Английский

Citations

4
Protocell Flow Reactors for Enzyme and Whole‐Cell Mediated Biocatalysis DOI Creative Commons

Huan Ma,

Xiayi Liu, Angela H. Nobbs

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(31)

Published: May 19, 2024

The design and construction of continuous flow biochemical reactors comprising immobilized biocatalysts have generated great interest in the efficient synthesis value-added chemicals. Living cells use compartmentalization reaction-diffusion processes for spatiotemporal regulation biocatalytic reactions, implementing these strategies into can offer new opportunities reactor application. Herein, fabrication protocell-based enzyme whole-cell mediated biocatalysis is demonstrated. Semipermeable membranized coacervate vesicles are employed as model protocells that spontaneously sequester enzymes or accumulate living bacteria to produce embodied microreactors capable single- multiple-step catalytic reactions. By packing millions enzyme/bacteria-containing a glass column, facile, cost-effective, modular methodology performing oxidoreductase, peroxidase lipolytic enzyme-mediated L-DOPA synthesis, glycolysis under conditions, It shown protocell-nested bacterial exhibit enhanced activities stability deleterious operating conditions compared with their non-encapsulated counterparts. These results provide step toward engineering based on cell-like microscale agents development green sustainable industrial bioprocessing.

Language: Английский

Citations

4
Facile Multilayer Deposition of Polyethylenimine and κ-Carrageenan on a 3D-Printed Lattice for Cellulase Immobilization DOI

Adrian Chiong Yuh Tiong,

Inn Shi Tan, Henry Chee Yew Foo

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(25), P. 9403 - 9414

Published: June 11, 2024

Technological developments for cellulase immobilization aim to address the challenges of costly cellulose hydrolysis. Herein, dissimilarly charged polyethylenimine (PEI) and κ-carrageenan (κ-Ca) were deposited layer-by-layer on a modular 3D-printed lattice immobilization, an enzyme loading improvement 54.9% was achieved after deposition 3 PEI/κ-Ca bilayers. Microscopy analyses revealed that layers increased average surface roughness by 65.4%, implying improved availability potential binding sites. Thermal stability reusability cellulase-loaded determined. They demonstrated activity retention 79.43% at 70 °C reaction temperature 83.97% ten enzymatic hydrolysis cycles with carboxymethylcellulose as substrate. Moreover, exhibited 64% higher turnover frequency than loaded nanosupports. Utilizing in macroalgae cellulosic residue yielded saccharification yield 44.91% 48 h. The synergisms between PEI κ-Ca improve stability, depicted from operational studies where E-GA-PkG showed ∼60% better free pH 7 using MCR Furthermore, flexibility prototyping complex geometries via 3D printing allows further intuition into more efficient reactor designs integrated biorefineries.

Language: Английский

Citations

2
Bioconversion of starch to Cyclodextrin using Cyclodextrin glycosyltransferase immobilized on metal organic framework DOI
Babatunde A. Ogunbadejo, Sulaiman Al‐Zuhair

Biocatalysis and Agricultural Biotechnology, Journal Year: 2023, Volume and Issue: 53, P. 102878 - 102878

Published: Oct. 1, 2023

Language: Английский

Citations

5
Functionalized corn stalk carboxymethyl cellulose and mesoporous silica SBA-15 composite hydrogel-immobilized lipase for the resolution of racemic ibuprofen ethyl ester DOI Creative Commons
Hong Zhang, Wenbin Liu, Lei Wang

et al.

Green Chemistry Letters and Reviews, Journal Year: 2024, Volume and Issue: 17(1)

Published: April 22, 2024

The immobilization of enzymes is an important strategy to improve their stability and reusability. However, it often comes with a trade off reduced biological activity catalytic efficiency. In this study, composite hydrogel based on corn stalk carboxymethyl cellulose, acrylic acid, mesoporous silica SBA-15 was developed as support for the lipase from Pseudomonas S.P. (PSL). surface morphology functional properties hydrogels were investigated by scanning electron microscopy, Fourier transform infrared, X-ray diffraction simultaneous thermal analyzer, respectively. materials had large number mesopores macropores present in wall sheet. network structure that diffusion resistance substrate improved accessibility enzyme. immobilized PSL exhibited high enantioselectivity (E value: 88.2) enzyme (316.8 µmol/g/min) preparation (S)-ibuprofen enzymatic hydrolytic resolution racemic ibuprofen ethyl ester. This prepared novel promising supporting matrix enzymes.

Language: Английский

Citations

1
Process intensification using immobilized enzymes DOI

Sera Bolat,

Raphael Greifenstein, Matthias Franzreb

et al.

Physical Sciences Reviews, Journal Year: 2023, Volume and Issue: 9(8), P. 2737 - 2755

Published: May 31, 2023

Abstract The application potential of enzymes is undoubtedly very high. However, despite the large number different and enzyme activities, industrial processes comparatively small. particular challenge often lies in transferring promising laboratory to an scale. Here, required performance parameters, such as stability or productivity, must be achieved. On one hand, this can achieved by improving enzymes. other key indicators only using technical systems sense process intensification. In enzymatic processes, immobilization means choice enable processes. aim article outline most important methods summarize immobilized Finally, are compared a case study with unspecific peroxygenase.

Language: Английский

Citations

1