Published: Jan. 1, 2024
Language: Английский
Environment International, Journal Year: 2024, Volume and Issue: 184, P. 108415 - 108415
Published: Jan. 8, 2024
An increasing number of harmful environmental factors are causing serious impacts on human health, and there is an urgent need to accurately identify the toxic effects mechanisms these factors. However, traditional toxicity test methods (e.g., animal models cell lines) often fail provide accurate results. Fortunately, organoids differentiated from stem cells can more accurately, sensitively specifically reflect body. They also suitable for specific studies frequently used in toxicology nowadays. As a combination organ-on-a-chip technology, organoids-on-a-chip has great potential toxicology. It controllable physicochemical microenvironment not easy be contaminated. higher homogeneity size shape organoids. In addition, it achieve vascularization exchange nutrients metabolic wastes time. Multi-organoids-chip simulate interactions different organs. These advantages facilitate better function maturity organoids, which make up shortcomings common certain extent. This review firstly discussed limitations testing platforms, leading introduction new platforms: organoids-on-a-chip. Next, applications were summarized prospected. Since platforms have been sufficiently considered previous literature, we particularly emphasized them. Finally, this opportunities challenges faced by organoids-on-a-chip, with expectation that readers will gain deeper understanding their value field
Language: Английский
Citations
18
Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(1)
Published: Jan. 1, 2024
Language: Английский
Citations
12
Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)
Published: March 5, 2024
Tumor organoids, especially patient-derived organoids (PDOs) exhibit marked similarities in histopathological morphology, genomic alterations, and specific marker expression profiles to those of primary tumour tissues. They are applied various fields including drug screening, gene editing, identification oncogenes. However, CAR-T therapy the treatment solid tumours is still at an exploratory stage. Tumour offer unique advantages over other preclinical models commonly used for research, which preservation biological characteristics tissue critical study early-stage therapies. Although some investigators have this co-culture model validate newly targeted cells, optimise existing cells explore combination strategies, there untapped potential today. This review introduces current status application organoid cell recent years commented on limitations co-cultivation model. Meanwhile, we compared with two pre-clinical research. Eventually, combined new progress technologies, optimization suggestions were proposed from five perspectives: preserving or reconstructing tumor microenvironment, systematization, vascularization, standardized culture procedures, expanding resource library, aimed assisting related researchers better utilize models.
Language: Английский
Citations
11
Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18
Published: July 9, 2024
In 2013, M. Lancaster described the first protocol to obtain human brain organoids. These organoids, usually generated from human-induced pluripotent stem cells, can mimic three-dimensional structure of brain. While they recapitulate salient developmental stages brain, their use investigate onset and mechanisms neurodegenerative diseases still faces crucial limitations. this review, we aim highlight these limitations, which hinder organoids becoming reliable models study such as Alzheimer’s disease (AD), Parkinson’s (PD), amyotrophic lateral sclerosis (ALS). Specifically, will describe structural biological impediments, including lack an aging footprint, angiogenesis, myelination, inclusion functional immunocompetent microglia—all important factors in neurodegeneration AD, PD, ALS. Additionally, discuss technical limitations for monitoring microanatomy electrophysiology parallel, propose solutions overcome current thereby making a more tool model neurodegeneration.
Language: Английский
Citations
10
MedComm, Journal Year: 2024, Volume and Issue: 5(10)
Published: Sept. 21, 2024
Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions specific organs. Although field organoids has experienced exponential growth, driven by advances in artificial intelligence, gene editing, bioinstrumentation, a comprehensive overview organoid applications remains necessary. This review offers detailed exploration historical origins characteristics various types, their applications-including disease modeling, drug toxicity efficacy assessments, precision medicine, regenerative medicine-as well as current challenges future directions research. have proven instrumental elucidating genetic cell fate hereditary diseases, infectious metabolic disorders, malignancies, study processes such embryonic development, molecular mechanisms, host-microbe interactions. Furthermore, integration technology with intelligence microfluidics significantly advanced large-scale, rapid, cost-effective thereby propelling progress medicine. Finally, advent high-performance materials, three-dimensional printing technology, also gaining prominence Our insights predictions aim to provide valuable guidance researchers support continued advancement this rapidly developing field.
Language: Английский
Citations
8
Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(21)
Published: Dec. 22, 2023
Organoids have emerged as major technological breakthroughs and novel organ models that revolutionized biomedical research by recapitulating the key structural functional complexities of their in vivo counterparts. The combination organoid systems microfluidic technologies has opened new frontiers engineering offers great opportunities to address current challenges existing broaden applications. In this review, features organoids, including origins, development, design principles, limitations, are described. Then recent progress integrating organoids into is highlighted, involving microarrays for high-throughput manipulation, microreactors hydrogel scaffold fabrication, chips culture. nascent microfluidics lie ahead accelerate disease studies, drug screening, regenerative medicine also discussed. Finally, future perspectives development advanced platforms modified building with higher fidelity standardization envisioned.
Language: Английский
Citations
15
Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 92, P. 103253 - 103253
Published: Jan. 14, 2025
Cerebral organoids pioneered in replicating complex brain tissue architectures vitro, offering a vast potential for human disease modeling. They enable the vitro study of physiological and pathophysiological mechanisms various neurological diseases disorders. The trajectory technological advancements organoid generation engineering over past decade indicates that technology might, future, mature into indispensable solutions at horizon personalized regenerative medicine. In this review, we highlight recent advances as models discuss some challenges opportunities future research rapidly evolving field.
Language: Английский
Citations
0
Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101323 - 101323
Published: April 1, 2025
Language: Английский
Citations
0
Nanomaterials, Journal Year: 2025, Volume and Issue: 15(6), P. 434 - 434
Published: March 12, 2025
Microfluidic technology has gained widespread application in the field of biomedical research due to its exceptional sensitivity and high specificity. Particularly when combined with nanomaterials, synergy between two significantly advanced fields such as precision medicine, drug delivery, disease detection, treatment. This article aims provide an overview latest achievements microfluidic nanomaterials detection It delves into applications detecting blood parameters, cardiovascular markers, neurological tumor markers. Special emphasis is placed on their roles treatment, including models vessels, blood–brain barrier, lung chips, tumors. The development emerging medical technologies, particularly skin interactive devices imaging, also introduced. Additionally, challenges future prospects current clinical are discussed. In summary, play indispensable role With continuous advancement technology, will become even more profound extensive.
Language: Английский
Citations
0