Phylogenetic and functional diversity amongDrosophila-associated metagenome-assembled genomes DOI Creative Commons
Aaron A. Comeault, Alberto H. Orta,

David P. Fidler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract Host-associated microbial communities can mediate interactions between their hosts and biotic abiotic environments. While much work has been done to document how microbiomes vary across species environments, less is known about the functional consequences of this variation. Here, we test for variation among drosophilid-associated bacteria by conducting Oxford Nanopore long-read sequencing generating metagenome-assembled genomes (MAGs) from six drosophilid fly collected in association with ‘anthropogenic’ environments North America, Europe, Africa. Using phylogenetic analyses, find that flies harbor a diverse microbiome includes core members closely related genera Gilliamella , Orbus Entomomonas Dysgonomonas others. Comparisons publicly available bacterial show many these are associated phylogenetically insect gut microbiomes. annotations predicted secondary metabolite biosynthetic gene clusters, MAGs belonging different orders content functions including metabolic capacity they respond environmental stressors. Our results provide evidence wild functionally communities. These findings highlight need quantify abundance function insect-associated others on performance

Language: Английский

Ginsenoside CK Alleviates DSS-Induced IBD in Mice by Regulating Tryptophan Metabolism and Activating Aryl Hydrocarbon Receptor via Gut Microbiota Modulation DOI
Yuan Liu, Xue Bai,

Huanyan Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 9867 - 9879

Published: April 11, 2024

Dysbiosis of gut microbiota is believed to be associated with inflammatory bowel disease (IBD). Ginsenoside compound K (CK), the main metabolite Panax ginseng ginsenoside, has proven effective as an anti-inflammatory agent in IBD. However, mechanisms by which CK modulates ameliorate IBD remain poorly understood. Herein, demonstrated potential suppress release proinflammatory cytokines modulation. Notably, supplementation promoted restoration a harmonious balance microbiota, primarily enhancing populations Lactobacillus and Akkermansia. Furthermore, considerably elevated concentrations tryptophan metabolites derived from that could activate aryl hydrocarbon receptor. Overall, promising alleviative efficacy stemmed promotion growth production metabolites, suggesting should regarded prospective prebiotic for future.

Language: Английский

Citations

11
The interactions between traditional Chinese medicine and gut microbiota in cancers: Current status and future perspectives DOI Creative Commons

Xuejiao Wei,

Fei Wang, Peng Tan

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 203, P. 107148 - 107148

Published: March 22, 2024

The gut microbiota, known as the "forgotten organ" and "human second genome," comprises a complex microecosystem. It significantly influences development of various tumors, including colorectal, liver, stomach, breast, lung cancers, through both direct indirect mechanisms. These mechanisms include "gut-liver" axis, "lung-intestine" interactions with immune system. intestinal flora exhibits dual roles in cancer, promoting suppressing its progression. Traditional Chinese medicine (TCM) can alter cancer progression by regulating flora. modifies flora's composition structure, along levels endogenous metabolites, thus affecting barrier, system, overall body metabolism. actions contribute to TCM's significant antitumor effects. Moreover, microbiota metabolizes TCM components, enhancing their properties. Therefore, exploring interaction between offers novel perspective understanding This paper succinctly reviews association gastric, cancers. further examines current research on flora, focus efficacy. identifies limitations existing studies suggests recommendations, providing insights into drug effectiveness. Additionally, this aims guide future studies.

Language: Английский

Citations

8
Sedanolide alleviates DSS-induced colitis by modulating the intestinal FXR-SMPD3 pathway in mice DOI Creative Commons
Shengjie Li,

Aoxiang Zhuge,

Hui Chen

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Inflammatory bowel disease (IBD) is a global with limited therapy. It reported that sedanolide exerts anti-oxidative and anti-inflammatory effects as natural phthalide, but its on IBD remain unclear. In this study, we investigated the impacts of dextran sodium sulfate (DSS)-induced colitis in mice. The mice were administered or vehicle followed by DSS administration, after which symptoms, inflammation levels, intestinal barrier function evaluated. Transcriptome analysis, 16S rRNA sequencing, targeted metabolomics analysis bile acids lipids performed. Sedanolide protected from DSS-induced colitis, suppressed inflammation, restored weakened epithelial barrier, modified gut microbiota decreasing salt hydrolase (BSH)-expressing bacteria. downregulation BSH activity increased ratio conjugated/unconjugated (BAs), thereby inhibiting farnesoid X receptor (FXR) pathway. roles FXR pathway verified using an FXR-specific agonist (fexaramine) germ-free mice, respectively. Furthermore, identified key effector ceramide, regulated sphingomyelin phosphodiesterase 3 (SMPD3). protective ceramide (d18:1/16:0) against demonstrated vitro human cell line Caco-2. could reshape flora influence BA composition, thus FXR-SMPD3 to stimulate synthesis ultimately alleviated Overall, our research revealed indicated may be clinical treatment for colitis. Additionally, lipid was shown mediate sedanolide, providing new insight into associations between metabolites.

Language: Английский

Citations

8
Microbial assistance in nano-carrier development: Innovative strategies in drug delivery DOI
Tejasvi Pandey, Vivek Pandey

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 95, P. 105607 - 105607

Published: March 22, 2024

Language: Английский

Citations

5
Analysis of intestinal bacterial carboxylesterase-mediated metabolites and the potential antitumour molecular mechanism of angoroside C DOI

Jian‐Ye Song,

Hui Xiang, Ru Feng

et al.

Journal of Asian Natural Products Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: Jan. 10, 2025

Angoroside C (AgrC) is a compound with many pharmacological properties. However, its antitumour potential has not been well studied. The low bioavailability of AgrC suggests strong link to gut bacteria. Therefore, we identified and quantified four metabolites in microbiota. Molecular docking inhibitor-based experiments demonstrated that carboxylesterase played key role metabolism. Both inhibited the viability CT-26 cells, targets were further explored. Additionally, significantly increased levels propionic, butyric, valeric isovaleric acids. This provides new insight for effects AgrC.

Language: Английский

Citations

0
Insights into the gut microbiota characteristics between the organic and traditional feeding chickens based on amplicon and metagenomic sequencing DOI Creative Commons
Wen‐Zhou Zhang, Jian Xiao,

Siqi Ding

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 23, 2025

Intestinal microorganisms play a crucial role in chicken health and production performance, especially the research of traditional organic feeding methods. The intestinal contents feed chickens were analyzed by 16S rRNA gene metagenome technology. results showed that microbial diversity was significantly higher than chickens, key microorganisms, such as Enterococcus , more abundant chickens. functional analysis revealed significant difference metabolic function between them. present study provides new insights into gut microbiota characteristics based on amplicon metagenomic sequencing. Our are helpful to fully illustrate effects different methods can offer scientific basis for management.

Language: Английский

Citations

0
Metabolic insights into gut microbiota in the pharmacology of natural medicines DOI
Zixin Chen,

Junchi Zhou,

Xiao Zheng

et al.

Chinese Journal of Natural Medicines, Journal Year: 2025, Volume and Issue: 23(2), P. 158 - 168

Published: Feb. 1, 2025

Language: Английский

Citations

0
A Comparative Study on Gut Microbiota and Metabolomics in Atlantic Salmon Salmo salar at Different Growth Stages DOI
Bowen Wang, X. Cao, Wenhao Ren

et al.

Journal of Ocean University of China, Journal Year: 2025, Volume and Issue: 24(2), P. 404 - 416

Published: March 10, 2025

Language: Английский

Citations

0
Progress on the mechanism of intestinal microbiota against colorectal cancer DOI Creative Commons
Guoqiang Xing,

Yu Cui,

Zhiyue Guo

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 28, 2025

The intestinal microbiota plays a crucial role in the occurrence and development of colorectal cancer, its anti - cancer mechanism has become research hotspot. This article comprehensively expounds on molecular mechanisms including aspects such as immune regulation, activation carcinogenic signaling pathways (it should be noted that it is more reasonable to "inhibition pathways"), metabolite mediated effects, maintenance barrier function. At same time, explores roles potential intervention methods probiotic supplementation therapy, immunotherapy, fecal transplantation. In addition, analyzes impact flora therapeutic efficacy cancer. existing results are summarized, future directions prospected, with aim providing new theoretical bases treatment ideas for prevention

Language: Английский

Citations

0
Gut Microbiome-Derived Metabolism Screen System Reveals Dehydrogenation Metabolism of Isorhynchophylline DOI
Lin Yang,

Zheng‐Meng Jiang,

Xin Liu

et al.

Journal of Natural Products, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Plant-derived natural products are commonly consumed orally and can be structurally modified by the gut microbiota, potentially altering their biological activity. However, high-throughput studies on microbial metabolism remain limited. This study applied an in vitro screening system for microbiome-derived using liquid chromatography-mass spectrometry to analyze of 200 human successfully annotated 118 metabolized microbiota. For first time, metabolic reactions isorhynchophylline wilforgine intestine were documented. Notably, a substantial increase plasma concentration isocorynoxeine was observed microbiota-colonized rats following administration compared those without colonization, suggesting that dehydrogenation microbiota also occurs vivo. 16S rRNA gene sequencing further revealed increased abundance Klebsiella Escherichia-Shigella administration, these genera may influence its transformation or pharmacological

Language: Английский

Citations

0