Liquid Chromatography with Orbitrap High-Resolution Mass Spectrometry for the Determination of Glucose in Saliva Following Derivatization with Dibenzylamine DOI

Zekun Tang,

Huihui Wan,

Huaishuan Zhang

et al.

Analytical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Aug. 14, 2024

As an important circulating metabolite in the body, accurate determination of glucose body fluids is very for evaluation physiological status. However, liquid chromatography–mass spectrometry (LC–MS) methods present challenges due to poor ionization combined with weak chromatographic retention. Here, a method determining salivary using LC–MS after derivation by dibenzylamine described which 3-O-methyl-D-glucopyranose was used as internal standard. Saliva samples were prepared protein precipitation, and analytes separated on polar-copolymerized C18 reversed phase column. Detection took place high-resolution orbitrap mass positive mode. The calibration function linear from 0.35 50 ng/mL. recoveries 97.82 104.51% intraday assays 98.96 106.61% interday assays. precision 0.37% 1.43% 1.33% 1.43%. limits detection quantification saliva 0.2 0.6 derivatization stored at 4 °C 25 analyzed every two days five repeats. relative standard deviations 0.09% 0.27% °C. This employed healthy male college students exercise.

Language: Английский

Glucose Sensing in Saliva DOI Creative Commons
Jacopo Giaretta, Riccardo Zulli,

Theja Prabhakar

et al.

Advanced Sensor Research, Journal Year: 2024, Volume and Issue: 3(11)

Published: July 29, 2024

Abstract Glucose plays critical roles in many human body functions, above all as a source of energy. Abnormal levels glucose are correlated to different diseases, importantly including diabetes. As such, quantification fluids is essential for health monitoring. Blood tests and, more recently, portable interstitial fluid tests, currently represent the benchmarks detection. Inconvenient invasive methods such blood pose burdens on both patients and healthcare system. In this review, noninvasive approaches measure discussed, utilizing saliva an alternative conventional samples. Techniques explored with potential enhance accuracy their associated challenges discussed.

Language: Английский

Citations

5
Colorimetric sweat analysis using wearable hydrogel patch sensors for detection of chloride and glucose DOI

Tuqiang Li,

Xiaofeng Chen, Ying Fu

et al.

Analytical Methods, Journal Year: 2023, Volume and Issue: 15(43), P. 5855 - 5866

Published: Jan. 1, 2023

Portable hydrogel-based wearable patch sensors were facilely prepared for colorimetric, real-time, rapid sweat analysis.

Language: Английский

Citations

11
Dental caries and salivary alterations in patients with type 2 diabetes: A systematic review and meta-analysis DOI
Ge Zhou,

Xingyue Shu,

Youlin Long

et al.

Journal of Dentistry, Journal Year: 2024, Volume and Issue: 150, P. 105321 - 105321

Published: Aug. 30, 2024

Language: Английский

Citations

4
An enzyme-free self-cascade catalytic dual-read biosensor for noninvasive detection of glucose based on oxidase-mimic Fe-MOF DOI
Xiulin Wang, Ziyi Guo, Tao Lian

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126334 - 126334

Published: May 1, 2025

Language: Английский

Citations

0
Liquid Chromatography with Orbitrap High-Resolution Mass Spectrometry for the Determination of Glucose in Saliva Following Derivatization with Dibenzylamine DOI

Zekun Tang,

Huihui Wan,

Huaishuan Zhang

et al.

Analytical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Aug. 14, 2024

As an important circulating metabolite in the body, accurate determination of glucose body fluids is very for evaluation physiological status. However, liquid chromatography–mass spectrometry (LC–MS) methods present challenges due to poor ionization combined with weak chromatographic retention. Here, a method determining salivary using LC–MS after derivation by dibenzylamine described which 3-O-methyl-D-glucopyranose was used as internal standard. Saliva samples were prepared protein precipitation, and analytes separated on polar-copolymerized C18 reversed phase column. Detection took place high-resolution orbitrap mass positive mode. The calibration function linear from 0.35 50 ng/mL. recoveries 97.82 104.51% intraday assays 98.96 106.61% interday assays. precision 0.37% 1.43% 1.33% 1.43%. limits detection quantification saliva 0.2 0.6 derivatization stored at 4 °C 25 analyzed every two days five repeats. relative standard deviations 0.09% 0.27% °C. This employed healthy male college students exercise.

Language: Английский

Citations

0