A fluorescence aptamer sensor utilizing WS2 nanosheets for sensitive detection of patulin: enhanced specificity and wide applicability DOI
Guoxin Qin, Huiling Li, Jie He

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(28), P. 4873 - 4879

Published: Jan. 1, 2024

A tungsten disulfide (WS2) nanosheet-based aptamer sensor was developed to detect patulin (PAT). The 5'-end of the PAT modified with a cyanine 3 (Cy3) fluorophore, which self-assembled on WS2 nanosheets. interaction between Cy3 fluorophore at and nanosheets resulted in reduced fluorescence (FL) intensity due resonance energy transfer (FRET). introduction into this sensing system led hybridization aptamer, forming G-quadruplex/PAT complex low affinity for nanosheet surface. This increased distance nanosheets, inhibiting FRET producing strong FL signal. Under optimal experimental conditions, demonstrated an excellent linear correlation concentrations ranging from 0.5 40.0 ng mL-1, it achieved detection limit (S/N = 3) 0.23 mL-1. offers enhanced specificity has potential broad application detecting other toxins by substituting sequence recognition aptamer.

Language: Английский

Bimetallic Fe3O4@Co3O4/CN as a Nanozyme with Dual Enzyme-Mimic Activities for the Colorimetric Determination of Mercury(II) DOI Creative Commons

Yanyan Xing,

Pingping He,

Deyong Wang

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(6), P. 104 - 104

Published: June 7, 2024

Colorimetric biosensor-based nanozymes have received considerable attention in various fields thanks to the advantages of simple preparation, good stability, and regulable catalytic activity nanozymes. In this study, a bimetallic nanozyme Fe3O4@Co3O4/CN was prepared via high-temperature calcination Fe3O4-PVP@ZIF-67. The material retained its skeletal structure before calcination, which prevented aggregation nanoparticles exposed more active sites nanozyme, substantially enhancing intrinsic dual enzyme-mimetic activities, including peroxidase- oxidase-like activities. particular, with catalyzed colorless tetramethylbenzidine (TMB) become blue oxTMB oxygen. Reducing glutathione (GSH) could inhibit above oxidation reaction. contrast, respect existence mercury(II), GSH bound mercury(II) due strong affinity between -SH, thus eliminating inhibition restoring signal. A effective colorimetric sensor fabricated detect based on principles. proposed measurement had linear range 0.1–15 μM limit detection (LOD) 0.017 μM. It shown that established sensing system be successfully applied water samples, proved promising candidate for biosensing application.

Language: Английский

Citations

3
Tunable Multi-Enzyme Activities of Platinum Nanoclusters for Enhanced Specificity and Sensitivity in Biosensing DOI

Jing Yang,

Jiayi Zhu,

Ruishu Xu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 283, P. 127173 - 127173

Published: Nov. 6, 2024

Language: Английский

Citations

3
Functional Evolution of Gold Nanoclusters under Light‐Induction: From Fluorescence to Multi‐Enzyme Mimetic Transition DOI Open Access
Jing Yang,

Ruishu Xu,

Haiyan Li

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Gold nanoclusters (AuNCs) exhibit unique physicochemical properties but their functional transitions under external stimuli remain underexplored. This study reveals that ultraviolet (UV) irradiation triggers structural evolution in AuNCs, causing fluorescence loss while activating dual enzyme-mimetic activities. UV-induced particle enlargement and surface restructuring endow AuNCs with peroxidase-like (POD-like) laccase-like functionalities. The POD-like activity enables sensitive glucose detection human serum, the capability facilitates simultaneous degradation of phenolic pollutants wastewater. These light-responsive transformations demonstrate AuNCs' dynamic adaptability, bridging intrinsic catalytic properties. findings elucidate photo-regulation mechanisms nanomaterial functionality, proposing a novel strategy for designing multifunctional nanosystems applicable biosensing environmental remediation. work advances development adaptive nanomaterials programmable features biomedical ecological applications.

Language: Английский

Citations

0
A fluorescence aptamer sensor utilizing WS2 nanosheets for sensitive detection of patulin: enhanced specificity and wide applicability DOI
Guoxin Qin, Huiling Li, Jie He

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(28), P. 4873 - 4879

Published: Jan. 1, 2024

A tungsten disulfide (WS2) nanosheet-based aptamer sensor was developed to detect patulin (PAT). The 5'-end of the PAT modified with a cyanine 3 (Cy3) fluorophore, which self-assembled on WS2 nanosheets. interaction between Cy3 fluorophore at and nanosheets resulted in reduced fluorescence (FL) intensity due resonance energy transfer (FRET). introduction into this sensing system led hybridization aptamer, forming G-quadruplex/PAT complex low affinity for nanosheet surface. This increased distance nanosheets, inhibiting FRET producing strong FL signal. Under optimal experimental conditions, demonstrated an excellent linear correlation concentrations ranging from 0.5 40.0 ng mL-1, it achieved detection limit (S/N = 3) 0.23 mL-1. offers enhanced specificity has potential broad application detecting other toxins by substituting sequence recognition aptamer.

Language: Английский

Citations

0