Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: April 26, 2024
Abstract
Toll-like
receptor
9
(TLR-9)
is
a
protein
that
helps
our
immune
system
identify
specific
DNA
types.
Upon
detection,
CpG
oligodeoxynucleotides
signal
the
to
generate
cytokines,
essential
proteins
contribute
body’s
defence
against
infectious
diseases.
Native
phosphodiester
type
B
ODNs
induce
only
Interleukin-6
with
no
effect
on
interferon-α.
We
prepared
silicon
quantum
dots
containing
different
surface
charges,
such
as
positive,
negative,
and
neutral,
using
amine,
acrylate-modified
Plouronic
F-127,
F-127.
Then,
class
are
loaded
of
SiQDs.
The
uptake
varies
based
charge;
positively
charged
SiQDs
demonstrate
higher
adsorption
compared
negative
neutral
charges.
level
cytokine
production
in
peripheral
blood
mononuclear
cells
was
found
be
associated
charge
prior
binding
ODNs.
Significantly
levels
IL-6
IFN-α
induction
were
observed
negatively
This
observation
strongly
supports
notion
effectively
regulates
induction.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(8), P. 931 - 931
Published: Aug. 1, 2024
Synthetic
oligodeoxynucleotides
(ODNs)
containing
unmethylated
cytosine-phosphate-guanine
(CpG)
motifs
(CpG-ODNs)
are
ligand
molecules
for
Toll-like
receptor
9
(TLR9),
which
is
expressed
by
odontoblasts
in
vitro
and
dental
pulp
cells.
This
study
determined
the
effects
of
CpG-ODNs
on
pulpal
immunomodulatory
response
repair
following
injury.
Briefly,
upper
right
first
molars
three-week-old
mice
were
extracted,
immersed
Type
A
(D35)
or
B
(K3)
CpG-ODN
solutions
(0.1
0.8
mM)
30
min,
then
replanted.
Pulpal
healing
activity
assessed
hematoxylin-eosin
AZAN
staining,
as
well
immunohistochemistry.
One
week
operation,
inflammatory
reactions
occurred
all
experimental
groups;
however,
re-revascularization
newly
formed
hard
tissue
deposition
observed
chamber
groups
at
2.
positive
trend
expression
immune
cell
markers
was
toward
0.1
mM.
Our
data
suggest
that
synthetic
low
concentrations
may
evoke
a
long-lasting
macrophage-TLR9-mediated
pro-inflammatory,
rather
than
anti-inflammatory,
to
modulate
process
formation.
Further
studies
needed
determine
current
agents
vivo
develop
treatment
strategies
regeneration.
Molecular Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 1, 2024
Unmethylated
cytosine-guanine
oligodeoxynucleotides
(CpG
ODNs)
have
a
storied
history
as
agonists
for
Toll-like
receptor
9
(TLR9).
CpG
ODNs
shown
promising
antitumor
effects
in
preclinical
studies
by
inducing
potent
proinflammatory
immune
responses.
However,
clinical
success
has
been
hindered
inconsistent
efficacy
and
immune-related
toxicities
caused
systemic
exposure
to
ODNs.
We
previously
identified
that
glatiramer
acetate
(GA),
an
FDA-approved,
lysine-rich
polypeptide,
could
complex
class
B
into
cationic
nanoparticles
which
persist
at
the
intratumoral
injection
site
while
mitigating
induction
of
cytokines
mouse
tumor
models.
To
extend
GA
applications
across
subtypes
ODN
(class
A,
B,
C),
we
evaluated
physiochemical
properties
immunological
signaling
its
complexes
with
different
classes
compared
characteristics
three
types
GA-CpG
nanoparticles,
followed
assessments
cell
uptake
efficiency
endolysosomal
trafficking.
then
performed
successive
vitro
vivo
assays
evaluate
discrepancies.
Complexation
preserved
activity
encapsulating
them
spherical
nanoparticles.
improved
cellular
ODNs,
generally
increased
retention
early
endosomes,
amplified
A
subsequent
experiment
confirmed
achievement
suppression
toxicities.
Together,
these
data
help
elucidate
noncanonical
role
serve
nucleic
acid
delivery
scaffold
can
improve
safety
adjuvant
cancer
immunotherapy.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: April 26, 2024
Abstract
Toll-like
receptor
9
(TLR-9)
is
a
protein
that
helps
our
immune
system
identify
specific
DNA
types.
Upon
detection,
CpG
oligodeoxynucleotides
signal
the
to
generate
cytokines,
essential
proteins
contribute
body’s
defence
against
infectious
diseases.
Native
phosphodiester
type
B
ODNs
induce
only
Interleukin-6
with
no
effect
on
interferon-α.
We
prepared
silicon
quantum
dots
containing
different
surface
charges,
such
as
positive,
negative,
and
neutral,
using
amine,
acrylate-modified
Plouronic
F-127,
F-127.
Then,
class
are
loaded
of
SiQDs.
The
uptake
varies
based
charge;
positively
charged
SiQDs
demonstrate
higher
adsorption
compared
negative
neutral
charges.
level
cytokine
production
in
peripheral
blood
mononuclear
cells
was
found
be
associated
charge
prior
binding
ODNs.
Significantly
levels
IL-6
IFN-α
induction
were
observed
negatively
This
observation
strongly
supports
notion
effectively
regulates
induction.
