Chemistry - A European Journal,
Journal Year:
2024,
Volume and Issue:
30(42)
Published: May 28, 2024
Abstract
Excited‐state
intramolecular
proton
transfer
(ESIPT)
molecules,
which
feature
large
Stokes
shifts
to
avoid
self‐absorption,
play
an
essential
role
in
photoluminescent
bioimaging
probes.
Herein,
we
report
the
development
of
ESIPT
molecule
3‐(3‐hydroxypyridin‐2‐yl)isoquinolin‐4‐ol
(
PiQ
).
not
only
undergoes
a
distinct
process
unlike
symmetrical
2,2′‐bipyridyl‐3,3′‐diol
but
also
exhibits
aggregation‐induced
emission
(AIE)
characteristics.
self‐assembles
into
aggregates
with
average
size
241.0±51.9
nm
aqueous
solutions,
leading
significantly
enhanced
photoluminescence.
On
basis
and
AIE
characteristics
,
latter
is
functionalized
hydrogen
peroxide‐responsive
4‐pinacoratoborylbenzyl
group
B
)
carboxylesterase‐responsive
acetyl
A
produce
probe
B−PiQ−A
.
The
potential
for
applications
chemical
sensing
underscored
by
its
efficient
detection
both
endogenous
exogenous
peroxide
living
cells.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Jan. 2, 2025
Bioorthogonal
chemistry-mediated
self-assembly
holds
great
promise
for
dynamic
molecular
imaging
in
living
organisms.
However,
existing
approaches
are
limited
to
nanoaggregates
with
'always-on'
signals,
suffering
from
high
signal-to-background
ratio
(SBR)
and
compromised
detection
sensitivity.
Herein
we
report
a
nitrile-aminothiol
(NAT)
bioorthogonal
fluorogenic
probe
(CyNAP-SS-FK)
ultrasensitive
diagnosis
of
orthotopic
hepatocellular
carcinoma.
This
comprises
nitrile-substituted
hemicyanine
scaffold
cysteine
tail
dually
locked
biomarker-responsive
moieties.
Upon
dual
cleavage
by
tumor-specific
cathepsin
B
biothiols,
the
1,2-aminothiol
residue
is
exposed
spontaneously
reacts
nitrile
group
situ
intramolecular
macrocyclization,
enabling
near-infrared
fluorescence
(NIRF)
turn-on
as
well
self-assembly.
In
male
mice,
such
'cleavage-click-assembly'
regimen
allows
real-time
small
cancerous
lesions
(~2
mm
diameter)
improved
SBR
(~5)
extended
window
(~36
h),
outperforming
conventional
clinical
assays.
study
not
only
presents
NAT
click
reaction-based
probes
but
also
highlights
generic
dual-locked
design
these
probes.
Existing
can
suffer
low
Here,
authors
sensing
action
lesions.
Macromolecules,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 10, 2025
Unconventional
fluorescent
polymers
have
attracted
wide
attention
due
to
their
excellent
biocompatibility,
facile
preparation,
and
unique
properties.
However,
developing
high-performance
revealing
the
emission
mechanism
are
still
challenging.
Herein,
explore
relationship
between
chemical
structures
properties,
four
hyperbranched
poly(silicophosphate)
esters
(HSiP0-HSiP3)
were
prepared.
Increasing
P═O(O)3:Si(O)3
ratios
generated
enhanced
red
fluorescence.
Experimental
theoretical
calculation
results
showed
that
n-π
interactions
P═O
O/N
atoms
promoted
concentration
of
negative
electrostatic
potential
spatial
electronic
communications,
then
decreased
energy
gap
strong
emission.
Meanwhile,
cluster
functional
groups
a
charge
isolated
groups,
which
further
Additionally,
these
in
bacterial
imaging
information
encryption.
Thus,
this
work
provides
an
efficient
method
for
long-wavelength
materials
while
providing
new
insights
into
origin.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 13, 2024
The
innovation
of
synthetic
strategies
for
selective
B-H
functionalization
is
a
pivotal
objective
in
the
realm
boron
cluster
chemistry.
However,
precise,
efficient,
and
rapid
bond
carboranes
that
distant
from
existing
functional
groups
remains
intractable
owing
to
limited
approaches
site-selective
control
established
methods.
Herein,
we
report
dative
bonding
activation
strategy
nonclassical
remote
site
Quarterly Reviews of Biophysics,
Journal Year:
2024,
Volume and Issue:
57
Published: Jan. 1, 2024
Abstract
Single-molecule
orientation-localization
microscopy
(SMOLM)
builds
upon
super-resolved
localization
by
imaging
orientations
and
rotational
dynamics
of
individual
molecules
in
addition
to
their
positions.
This
added
dimensionality
provides
unparalleled
insights
into
nanoscale
biophysical
biochemical
processes,
including
the
organization
actin
networks,
movement
molecular
motors,
conformations
DNA
strands,
growth
remodeling
amyloid
aggregates,
composition
changes
within
lipid
membranes.
In
this
review,
we
discuss
recent
innovations
SMOLM
cover
three
key
aspects:
(1)
enabled
labeling
strategies
that
endow
fluorescent
probes
bind
targets
with
orientation
specificity;
(2)
advanced
techniques
leverage
physics
light-matter
interactions
estimation
theory
encode
information
high
fidelity
microscope
images;
(3)
computational
methods
ensure
accurate
precise
data
analysis
interpretation,
even
presence
severe
shot
noise.
Additionally,
compare
approaches,
hardware,
publicly
available
software
aid
community
choosing
best
implementation
for
specific
application.
Finally,
highlight
future
directions
SMOLM,
such
as
development
improved
photostability
specificity,
design
“smart”
adaptive
use
approaches
handle
large,
complex
datasets.
review
underscores
significant
current
potential
impact
deepening
our
understanding
dynamics,
paving
way
breakthroughs
fields
biophysics,
biochemistry,
materials
science.
Chemical & Biomedical Imaging,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 4, 2025
Aggregation-induced
emission
luminogens
(AIEgens)
have
been
prosperously
developed
and
applied
in
the
fields
of
optical
imaging
theranostics
since
its
establishment.
Nowadays,
AIEgens
can
fulfill
nearly
all
requirements
with
spectra
ranging
from
visible
to
near-infrared
wavelengths.
Although
a
variety
varying
wavelengths
functionalities
continuously
designed,
their
performance
is
heavily
dependent
on
use
conventional
light
sources,
such
as
xenon
lamps
lasers,
which
severely
hinder
further
applications
due
limited
penetration
depth
background
autofluorescence
biological
tissues.
To
mitigate
these
limitations
maximize
potential
AIEgens,
unconventional
excitation
sources
chemical
energy,
ultrasound,
X-ray
offer
effective
alternatives
that
circumvent
drawbacks
associated
traditional
light-based
constant
excitation.
In
this
Review,
we
introduce
fundamental
principles
governing
combination
highlight
recent
advancements
using
excited
by
for
bioimaging
theranostics,
discuss
current
challenges
future
perspectives
aimed
at
advancing
biomedical
AIEgens.
