p-hydroxy benzaldehyde attenuates intestinal epithelial barrier dysfunction caused by colitis via activating the HNF-1β/SLC26A3 pathway DOI Creative Commons
Meng Liu, Yuhui Wang, Xiaotian Xu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 22, 2024

Background Intestinal epithelial barrier dysfunction is intricately linked to the pathogenesis of ulcerative colitis (UC). Dietary interventions that bolster intestinal function can effectively thwart UC onset. Our prior research revealed p-Hydroxy benzaldehyde (HD), a phenolic compound from Nostoc commune (an edible cyanobacterium), markedly upregulated expression E-cadherin, pivotal protein in mucosa, thereby mitigating mucosal damage mice afflicted with dextran sulfate sodium (DSS)-induced colitis. Nevertheless, precise molecular mechanisms underpinning HD’s ameliorative effects on remain elusive. Methods Dextran mouse model was established, and successful establishment determined by evaluating changes body weight, disease activity index (DAI), colonic histopathology, white blood cell count. Transmission electron microscopy (TEM) observed ultrastructural villi. The levels inflammatory factors ( IFN-γ IL-13 ) permeability indicators (FITC-Dextran, DAO, ET, D-LA were detected Enzyme-linked immunosorbent assay (ELISA). Western blotting (WB) immunohistochemistry (IHC) used detect integrity-related such as tight junction TJs (ZO-1, occludin) adhesion AJs (E-cadherin). Furthermore, WB, Pull-down assay, drug affinity reaction target stability (DARTS) docking dynamics (MD) simulation determine potential mechanism HD. Results HD intervention significantly alleviated symptoms mice, inhibited weight loss colon shortening, reduced DAI score pathological score, maintained ultrastructure villi tissue, IFN-γ, number cells tissue mice. could also reduce FITC-Dextran, increase ZO-1, occludin, E-cadherin tissues maintaining impaired caused Mechanically, augmented hepatocyte nuclear factor 1β (HNF-1β) DRA. Adeno-associated virus (AAV)-HNF-1β shRNA or Lentivirus-mediated HNF-1β knockdown abolished HD-induced protection, well promotion solute carrier family 26 member 3 (SLC26A3) levels. SLC26A3 siRNA reversed inhibition DARTS analysis, docking, MD results showed high binding strength, interaction efficiency remarkable between Conclusion This study elucidates role forestalling disruption under conditions. Mechanistic investigations fortifies via HNF-1β/SLC26A3 pathway, thus preserving lower barrier’s integrity UC.

Language: Английский

The role of bound polyphenols in the anti-obesity effects of defatted rice bran insoluble dietary fiber: An insight from multi-omics DOI
Bing Zheng,

Xiaole Zhao,

Tianxiang Ao

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 459, P. 140345 - 140345

Published: July 5, 2024

Language: Английский

Citations

7
WKB ameliorates DSS-induced colitis through inhibiting enteric glial cells activation and altering the intestinal microbiota DOI Creative Commons
Qi Sun, Bai‐Rong Li, Di Li

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 21, 2025

Language: Английский

Citations

1
The probiotic Lactobacillus plantarum alleviates colitis by modulating gut microflora to activate PPARγ and inhibit MAPKs/NF-κB DOI

Rongxin Zang,

Rui Zhou,

Yaodong Li

et al.

European Journal of Nutrition, Journal Year: 2024, Volume and Issue: 64(1)

Published: Nov. 28, 2024

Language: Английский

Citations

4
Candida utilis Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice via NF-κB/MAPK Suppression and Gut Microbiota Modulation DOI Open Access

Rongxin Zang,

Zhouliang Liu,

Huihao Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1993 - 1993

Published: Feb. 25, 2025

Candida utilis (CUM) possesses various biological effects, including anti-inflammatory, intestinal microbiota regulatory, and immunomodulatory activities. However, there has been little exploration regarding the effects of CUM on ulcerative colitis (UC). Therefore, this study aimed to investigate beneficial alleviating dextran sulfate sodium (DSS)-induced UC in mice explore potential underlying mechanisms. Here, effect was analyzed using a DSS-induced mouse model (n = 9), results which indicated decrease disease activity index (DAI) mice. Furthermore, alleviated colon shortening, minimized tissue damage, preserved tight junction proteins (Claudin-3, Occludin, ZO-1). reduced level pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), inhibited activation NF-ĸB, MAPK PPARγ signaling pathways, decreased oxidative mediators (MPO, SOD MDA) Additionally, it mitigated dysbiosis by increasing abundance Prevotellaceae Lactobacillus while decreasing Bacteroidaceae Enterobacteriaceae. short-chain fatty acids (SCFAs) content The above provide scientific basis for CUM, as natural supplement, restore balance gut inflammatory promote health.

Language: Английский

Citations

0
Effect of various extraction methods on the physicochemical properties, antioxidant, and anti-inflammatory activities of mung bean (Vigna radiata L.) skin polysaccharides DOI
Xiao Ding, Tingting Li, Jiajia Zhao

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143969 - 143969

Published: May 5, 2025

Language: Английский

Citations

0
Soluble Dietary Fiber from Fermentation of Tea Residues by Eurotium cristatum and the Effects on DSS-Induced Ulcerative Colitis DOI
Maiquan Li, Wenlan Li,

Manjun Kang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Tea residues are rich in dietary fiber, most of which insoluble fiber (IDF). However, soluble (SDF) is reported to show a better health-promoting effect. In this paper, the Eurotium cristatum (E. cristatum) fermentation method was employed prepare SDF from tea residues. The results showed that yield fermented (FSDF) higher than unfermented (USDF). Meanwhile, an increased proportion galactose and looser microstructure were observed FSDF. addition, FSDF has more advantages USDF relieving colitis symptoms. effective reversing weight loss, colon shortening, tissue damage. it regulatory effect on level inflammatory factors (IL-6, IL-1β, TNF-α, IL-10) oxidative stress (CAT, T-SOD, MDA). treatment effectively restored gut microbiota composition toward normal parameters compared by upregulating Akkermanisa Lachospiracae_NK4A136_group downregulating Helicobacter Alisitipes. conclusion, with E. contributed preparation efficiency bioactive This study will provide theoretical basis for development utilization

Language: Английский

Citations

0
Lactobacillus paracasei Jlus66 relieves DSS-induced ulcerative colitis in a murine model by maintaining intestinal barrier integrity, inhibiting inflammation, and improving intestinal microbiota structure DOI

Fazheng Yu,

Xiaoxu Wang, Honglin Ren

et al.

European Journal of Nutrition, Journal Year: 2024, Volume and Issue: 63(6), P. 2185 - 2197

Published: May 11, 2024

Language: Английский

Citations

1
p-hydroxy benzaldehyde attenuates intestinal epithelial barrier dysfunction caused by colitis via activating the HNF-1β/SLC26A3 pathway DOI Creative Commons
Meng Liu, Yuhui Wang, Xiaotian Xu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 22, 2024

Background Intestinal epithelial barrier dysfunction is intricately linked to the pathogenesis of ulcerative colitis (UC). Dietary interventions that bolster intestinal function can effectively thwart UC onset. Our prior research revealed p-Hydroxy benzaldehyde (HD), a phenolic compound from Nostoc commune (an edible cyanobacterium), markedly upregulated expression E-cadherin, pivotal protein in mucosa, thereby mitigating mucosal damage mice afflicted with dextran sulfate sodium (DSS)-induced colitis. Nevertheless, precise molecular mechanisms underpinning HD’s ameliorative effects on remain elusive. Methods Dextran mouse model was established, and successful establishment determined by evaluating changes body weight, disease activity index (DAI), colonic histopathology, white blood cell count. Transmission electron microscopy (TEM) observed ultrastructural villi. The levels inflammatory factors ( IFN-γ IL-13 ) permeability indicators (FITC-Dextran, DAO, ET, D-LA were detected Enzyme-linked immunosorbent assay (ELISA). Western blotting (WB) immunohistochemistry (IHC) used detect integrity-related such as tight junction TJs (ZO-1, occludin) adhesion AJs (E-cadherin). Furthermore, WB, Pull-down assay, drug affinity reaction target stability (DARTS) docking dynamics (MD) simulation determine potential mechanism HD. Results HD intervention significantly alleviated symptoms mice, inhibited weight loss colon shortening, reduced DAI score pathological score, maintained ultrastructure villi tissue, IFN-γ, number cells tissue mice. could also reduce FITC-Dextran, increase ZO-1, occludin, E-cadherin tissues maintaining impaired caused Mechanically, augmented hepatocyte nuclear factor 1β (HNF-1β) DRA. Adeno-associated virus (AAV)-HNF-1β shRNA or Lentivirus-mediated HNF-1β knockdown abolished HD-induced protection, well promotion solute carrier family 26 member 3 (SLC26A3) levels. SLC26A3 siRNA reversed inhibition DARTS analysis, docking, MD results showed high binding strength, interaction efficiency remarkable between Conclusion This study elucidates role forestalling disruption under conditions. Mechanistic investigations fortifies via HNF-1β/SLC26A3 pathway, thus preserving lower barrier’s integrity UC.

Language: Английский

Citations

1