Disposable capillary-fill device for the determination of proteases incorporating elimination of light-shielding from the magnetic beads with cleavage of the electrogenerated chemiluminescence label-tagged peptide probe DOI
Xiaolin Yang,

Jie Li,

Honglan Qi

et al.

The Analyst, Journal Year: 2023, Volume and Issue: 148(24), P. 6253 - 6260

Published: Jan. 1, 2023

A novel point-of-care testing (POCT) method for the determination of proteases was developed first time using a designed disposable capillary-fill device based on cleavage electrogenerated chemiluminescence (ECL)-label-tagged peptide probes and enabling elimination light-shielding from magnetic beads (MBs). As proof-of-principle, prostate-specific antigen (PSA) taken as model analyte, streptavidin-coated bound with ruthenium-complex-tagged specific (biotin-HSSKLQK) were utilized MB ECL probes. The to be divided into reaction zone detection zone. In zone, bio-cleavage between PSA analyte surface occurred, while in emission produced by screen-printed carbon electrode, Ag/AgCl reference electrode counter electrode. When introduced suspension device, biocleavage cleaved Ru1 part released capillary-filled tilted 90°, aid gravity, solution containing flowed working region fixed an external magnet. can determined presence co-reactant tri-n-propylamine at Under optimal conditions, showed low limit 0.12 ng mL-1 PSA. This work demonstrates that biosensing approach eliminate effect quantify high sensitivity selectivity, which could easily extended POCT-based other proteases.

Language: Английский

Air sampling and ATP bioluminescence for quantitative detection of airborne microbes DOI
Qing Liu, Saisai Yan, Miao Zhang

et al.

Talanta, Journal Year: 2024, Volume and Issue: 274, P. 126025 - 126025

Published: March 31, 2024

Language: Английский

Citations

13
Electrochemical Sensors in the Food Sector: A Review DOI
Masoud Ghaani, Mostafa Azimzadeh, Duygu Büyüktaş

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(44), P. 24170 - 24190

Published: Oct. 25, 2024

In a world that is becoming increasingly concerned with health, safety, and the sustainability of food supply chains, control assurance quality have become utmost importance. This review examines application potential electrochemical sensors in dynamic field science to meet these expanding demands. The article introduces describes their operational mechanics components contributing function. A summary most prevalent methods outlines diverse analysis techniques available. shifts discussing applications sensors, highlighting crucial role detecting compounds samples like meat, fish, juice, milk for contemporary control. paper showcases sensors' utility analysis, underscoring significance as powerful, efficient tools maintaining safety how they could transform our approach global assurance.

Language: Английский

Citations

13
Nanosensors Based on Breathomics for Human Disease Diagnosis: a New Frontier in Personalized Healthcare DOI Creative Commons
Bakr Ahmed Taha, Ali J. Addie, Adawiya J. Haider

et al.

BioNanoScience, Journal Year: 2025, Volume and Issue: 15(2)

Published: March 14, 2025

Language: Английский

Citations

0
Enzyme-free electrochemical sensor platforms based on transition metal nanostructures for clinical diagnostics DOI
Govindhan Maduraiveeran

Analytical Methods, Journal Year: 2023, Volume and Issue: 15(48), P. 6620 - 6630

Published: Jan. 1, 2023

The detection of emergent biomarkers is key significance in numerous clinical, biological, and biomedical fields. Specifically, the design development potent electrochemical lactic acid glucose sensing platforms are especially great demand a variety industries, including those involved clinical analysis, biomedicine, food, cosmetics, pharmaceuticals, leather, sports, chemical industries. Nanostructured transition metal-derived materials have opened door to sensors biosensors due their advantages high surface-to-volume ratio, surface reaction activity, catalytic strong adsorption capability. primary aim present minireview highlight advancement enzyme-free sensor based on nanostructures with electrocatalytic activity performance towards practical samples. preparation approaches, structural composition monitoring, fabrication electrodes, real samples, exploration mechanisms majorly concentrated most our recent research studies. Moreover, state-of-the-art nanostructure-derived platforms, critical comparison analytical future perspectives for diagnostics described.

Language: Английский

Citations

5
Disposable capillary-fill device for the determination of proteases incorporating elimination of light-shielding from the magnetic beads with cleavage of the electrogenerated chemiluminescence label-tagged peptide probe DOI
Xiaolin Yang,

Jie Li,

Honglan Qi

et al.

The Analyst, Journal Year: 2023, Volume and Issue: 148(24), P. 6253 - 6260

Published: Jan. 1, 2023

A novel point-of-care testing (POCT) method for the determination of proteases was developed first time using a designed disposable capillary-fill device based on cleavage electrogenerated chemiluminescence (ECL)-label-tagged peptide probes and enabling elimination light-shielding from magnetic beads (MBs). As proof-of-principle, prostate-specific antigen (PSA) taken as model analyte, streptavidin-coated bound with ruthenium-complex-tagged specific (biotin-HSSKLQK) were utilized MB ECL probes. The to be divided into reaction zone detection zone. In zone, bio-cleavage between PSA analyte surface occurred, while in emission produced by screen-printed carbon electrode, Ag/AgCl reference electrode counter electrode. When introduced suspension device, biocleavage cleaved Ru1 part released capillary-filled tilted 90°, aid gravity, solution containing flowed working region fixed an external magnet. can determined presence co-reactant tri-n-propylamine at Under optimal conditions, showed low limit 0.12 ng mL-1 PSA. This work demonstrates that biosensing approach eliminate effect quantify high sensitivity selectivity, which could easily extended POCT-based other proteases.

Language: Английский

Citations

2